Anastrozole improved testosterone-induced impairment acquisition of spatial learning and memory in the hippocampal CA1 region in adult male rats

• Published in: Behavioural brain research Vol. 175; no. 2; pp. 223 - 232
• Main Authors: Moradpour, Farshad, Naghdi, Nasser, Fathollahi, Yaghob
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 15.12.2006; Elsevier Science
• Subjects: Analysis of Variance; Anastrozole; Animal; Animals; Aromatase Inhibitors - pharmacology; Behavioral psychophysiology; Biological and medical sciences; CA1 region; Discrimination Learning - drug effects; Discrimination Learning - physiology; Escape Reaction - drug effects; Escape Reaction - physiology; Estradiol - administration & dosage; Estradiol - analogs & derivatives; Estradiol valerat; Fundamental and applied biological sciences. Psychology; Hippocampus; Hippocampus - drug effects; Hippocampus - enzymology; Hormones and behavior; Learning. Memory; Male; Maze Learning - drug effects; Maze Learning - physiology; Memory; Memory Disorders - chemically induced; Memory Disorders - enzymology; Microinjections; Nitriles - pharmacology; Psychology. Psychoanalysis. Psychiatry; Psychology. Psychophysiology; Rats; Rats, Wistar; Reaction Time - drug effects; Reaction Time - physiology; Spatial Behavior - drug effects; Spatial Behavior - physiology; Statistics, Nonparametric; Testosterone - administration & dosage; Testosterone - physiology; Testosterone enanthate; Triazoles - pharmacology; CA1 region; Testosterone enanthate; Estradiol valerat; Anastrozole; Hippocampus; Antineoplastic agent; Rat; Antiestrogen; Estrogen; Central nervous system; Male; Cognition; Space perception; Antihormone; Estrogen synthase; Estradiol; Encephalon; Acquisition process; Testicular hormone; Non steroid compound; Androgen; Enzyme; Rodentia; Enzyme inhibitor; Ovarian hormone; Testosterone; Vertebrata; Mammalia; Adult animal; Triazole derivatives; Sex steroid hormone; Perceptive learning; Spatial memory
• DOI: 10.1016/j.bbr.2006.08.037
• ISSN: 0166-4328

Neurohormones like testosterone and estrogen have an important role in learning and memory. Many biological effects of androgens in the brain require the local conversion of these steroids to an estrogen. The current research has conducted to assess the effect of testosterone, estrogen and aromatase inhibitor (anastrozole) on spatial discrimination of rats, using Morris water maze and also the pathway of the effect of testosterone by using anastrozole. Adult male rats were bilaterally cannulated into CA1 region of hippocampus and divided into 15 groups. Different groups received DMSO 0.5 μl and DMSO 0.5 μl + DMSO 0.5 μl as control groups and different doses of testosterone enanthate (TE) (20, 40 and 80 μg/0.5 μl), estradiol valerat (EV) (1, 2.5, 5, 10, and 15 μg/0.5 μl), anastrozole (An) (0.25, 0.5, 1 μg/0.5 μl), TE 80 μg/0.5 μl + anastrozole 0.5 μg/0.5 μl and EV 15 μg/0.5 μl + anastrozole 0.5 μg/0.5 μl all days before training. TE and EV were injected 30–35 min before training and anastrozole was injected 25–30 min before training. Our results have shown both TE 80 μg/0.5 μl and EV 15 μg/0.5 μl groups increase in escape latency and traveled distance to find invisible platform. Also we have shown that anastrozole dose dependently decreases escape latency and traveled distance. We resulted that both TE and EN impaired acquisition of spatial learning and memory but anastrozole improved it. Anastrozole also could be buffered TE-induced impairment effect but not EV.