UHPLC–MS/MS method for the quantitation of penicillin G and metabolites in citrus fruit using internal standards

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 1044-1045; pp. 87 - 94
• Main Authors: Canzani, Daniele, Hsieh, Kevin, Standland, Matthew, Hammack, Walter, Aldeek, Fadi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.02.2017
• Subjects: Chromatography, High Pressure Liquid - methods; Chromatography, High Pressure Liquid - standards; Citrus - chemistry; Fruit - chemistry; Huanglongbing (HLB); Internal standards; LC–MS/MS; Limit of Detection; Linear Models; Metabolites; Method development; Penicillin G; Penicillin G - analysis; Penicillin G - chemistry; Penicillin G - metabolism; Reference Standards; Reproducibility of Results; Tandem Mass Spectrometry - methods; Tandem Mass Spectrometry - standards; Huanglongbing (HLB); Internal standards; Metabolites; Method development; Penicillin G; LC–MS/MS

•Development and validation of LC–MS/MS method to identify and quantitate penicillin G and two of its metabolites, penillic acid and penilloic acid, in citrus fruit.•Improvement of a previous method by incorporating isotopically labeled internal standards, namely, penillic acid-D5, and penilloic aci...