Protein encapsulation by electrospinning and electrospraying

• Published in: Journal of controlled release Vol. 329; pp. 1172 - 1197
• Main Authors: Moreira, Anabela, Lawson, Dan, Onyekuru, Lesley, Dziemidowicz, Karolina, Angkawinitwong, Ukrit, Costa, Pedro F., Radacsi, Norbert, Williams, Gareth R.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 10.01.2021
• Subjects: Drug delivery; Electrospinning; Electrospraying; Protein encapsulation; Tissue engineering; FDA; ALP; HRP; Electrospinning; Drug delivery; CTGF; Protein encapsulation; BSA; Electrospraying; FITC; IFN-γ; LSCM; TGF-β; MSC; EC; CD; GI; BMP; tPA; Tissue engineering; EGF; VEGF; SMC; IGF-1; GAG; NGF; bFGF; CAM; ECM; SDS-PAGE; TE; GDNF; TNF-α; PDGF; LbL; DSDP; OVA; ELISA
• ISSN: 0168-3659; 1873-4995
• DOI: 10.1016/j.jconrel.2020.10.046

Given the increasing interest in the use of peptide- and protein-based agents in therapeutic strategies, it is fundamental to develop delivery systems capable of preserving the biological activity of these molecules upon administration, and which can provide tuneable release profiles. Electrohydrodynamic (EHD) techniques, encompassing electrospinning and electrospraying, allow the generation of fibres and particles with high surface area-to-volume ratios, versatile architectures, and highly controllable release profiles. This review is focused on exploring the potential of different EHD methods (including blend, emulsion, and co−/multi-axial electrospinning and electrospraying) for the development of peptide and protein delivery systems. An overview of the principles of each technique is first presented, followed by a survey of the literature on the encapsulation of enzymes, growth factors, antibodies, hormones, and vaccine antigens using EHD approaches. The possibility for localised delivery using stimuli-responsive systems is also explored. Finally, the advantages and challenges with each EHD method are summarised, and the necessary steps for clinical translation and scaled-up production of electrospun and electrosprayed protein delivery systems are discussed. [Display omitted] •Electrospinning and electrospraying can be used for protein encapsulation.•Use of biocompatible, FDA-approved materials may facilitate clinical translation.•Coaxial architectures offer advantages over blend and emulsion techniques.•Preservation of bioactivity and sustained release profiles can be achieved.•Potential for scaled-up manufacturing paves the way for product commercialisation.