Dynamics of component exchange at PML nuclear bodies

• Published in: Journal of cell science Vol. 121; no. 16; pp. 2731 - 2743
• Main Authors: Weidtkamp-Peters, Stefanie, Lenser, Thorsten, Negorev, Dmitri, Gerstner, Norman, Hofmann, Thomas G, Schwanitz, Georg, Hoischen, Christian, Maul, Gerd, Dittrich, Peter, Hemmerich, Peter
• Format: Journal Article
• Language: English
• Published: England The Company of Biologists Limited 15.08.2008
• Subjects: Antigens, Nuclear - metabolism; Autoantigens - metabolism; Cell Nucleus - metabolism; Cells, Cultured; Diffusion; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; HeLa Cells; Humans; Intranuclear Inclusion Bodies - metabolism; Kinetics; Models, Biological; Nuclear Proteins - chemistry; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Oncogene Proteins, Fusion - metabolism; Promyelocytic Leukemia Protein; Protein Binding; Protein Isoforms - metabolism; Protein Processing, Post-Translational - physiology; Protein Structure, Tertiary - physiology; Protein Transport - physiology; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Substrate Specificity; SUMO-1 Protein - metabolism; Transcription Factors - chemistry; Transcription Factors - genetics; Transcription Factors - metabolism; Tumor Suppressor Proteins - chemistry; Tumor Suppressor Proteins - genetics; Tumor Suppressor Proteins - metabolism

PML nuclear bodies (NBs) are involved in the regulation of key nuclear pathways but their biochemical function in nuclear metabolism is unknown. In this study PML NB assembly dynamics were assessed by live cell imaging and mathematic modeling of its major component parts. We show that all six nuclea...