LC-MS/MS screening and identification of bioactive compounds in leaves, pulp and seed from Eugenia calycina Cambess

• Published in: Food research international Vol. 137; p. 109556
• Main Authors: Peixoto Araujo, Nayara Macêdo, Arruda, Henrique Silvano, dos Santos, Fábio Neves, de Morais, Damila Rodrigues, Pereira, Gustavo Araujo, Pastore, Glaucia Maria
• Format: Journal Article
• Language: English
• Published: Canada Elsevier Ltd 01.11.2020
• Subjects: Brazil; Chromatography, Liquid; Eugenia; Eugenia species; Flavonoids; Humans; Phenolic acids; Phytochemical composition; Pitanga-do-cerrado; Plant Extracts; Plant Leaves; Seeds; Tandem Mass Spectrometry; Brazil; Flavonoids; Eugenia species; Pitanga-do-cerrado; Phenolic acids; Phytochemical composition
• ISSN: 0963-9969; 1873-7145
• DOI: 10.1016/j.foodres.2020.109556

[Display omitted] •Eugenia calycina showed a high content of phenolic compounds.•Ellagic acid, myricitrin and gallocatechin were the main phenolic compounds.•Leaf and seed contained mainly flavonoids and pulp had especially phenolic acids.•LC-MS/MS-based untargeted identified 139 phytochemicals.•Eugenia calycina can be a novel source of phenolic compounds. The Eugenia calycina Cambess, also known as pitanga-do-cerrado, is an unexplored Brazilian fruit used by native people for the treatment of diabetes. The aim of this study was to identify the phenolic compounds from the leaves, pulp and seed of Eugenia calycina by using LC-MS-based targeted and untargeted analysis. The LC-MS-based targeted quantitative analysis showed a high phenolic content in all plant parts in which the ellagic acid was the main phenolic compound with values of 8244.53 µg/g dw (leaves), 5054.43 µg/g dw (pulp) and 715.42 µg/g dw (seed). The leaves, pulp and seed showed a high total phenolic content of 20371.96, 7139.70 and 2204.75 µg/g dw, respectively. In addition, the LC-MS-based targeted analysis showed ellagic acid, myricitrin and epicatechin gallate as the main phenolic compounds in the Eugenia calycina. The LC-MS-based untargeted analysis showed the phytochemical profile of the leaves, pulp and seed in which 153 phytochemicals from different chemical classes were annotated, including organic acids, phenolic acids, flavonoids, and other compounds. The Eugenia calycina has high potential as a plant-based food due to its phytochemical profile and high phenolic content.