Protective effects of Vitamin E on endocrine disruptors, PCB-induced dopaminergic neurotoxicity

• Published in: Toxicology (Amsterdam) Vol. 216; no. 2; pp. 140 - 146
• Main Authors: Yun, Jae Suk, Na, Han Kwang, Park, Ki-Sook, Lee, Yun Hee, Kim, Eun Yeob, Lee, Sung Yong, Kim, Joo-Il, Kang, Ju-Hee, Kim, Dong Sup, Choi, Ki Hwan
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 15.12.2005; Amsterdam Elsevier Science
• Subjects: Administration, Oral; Animals; Aroclor 1254; Biological and medical sciences; Cell Culture Techniques; Cell Survival - drug effects; Chromatography, High Pressure Liquid; Corpus Striatum - drug effects; Corpus Striatum - metabolism; dl-α-Tocopherol (Vitamin E); Dopamine - metabolism; Endocrine Disruptors; Immunoblotting; Male; Medical sciences; Microdialysis; Neuroprotective; Neurotoxicity Syndromes - etiology; Neurotoxicity Syndromes - metabolism; Neurotoxicity Syndromes - prevention & control; Nitric oxide synthase; Nitric Oxide Synthase Type I - drug effects; Nitric Oxide Synthase Type I - metabolism; Phosphorylation - drug effects; Polychlorinated Biphenyls - antagonists & inhibitors; Polychlorinated Biphenyls - toxicity; Rats; Rats, Sprague-Dawley; Time Factors; Toxicology; Up-Regulation - drug effects; Vitamin E - administration & dosage; Vitamin E - pharmacology; Vitamin E - therapeutic use; Aroclor 1254; Neuroprotective; Nitric oxide synthase; dl-α-Tocopherol (Vitamin E); Microdialysis; Neuroprotection; Nervous system diseases; Enzyme; Toxicity; DL-α-Tocopherol (Vitamin E); α-Tocopherol; Neuroprotective agent; Endocrine disruptor; E-Vitamins; Antioxidant; Polychlorobiphenyl; Nitric-oxide synthase; Prevention; Oxidoreductases
• ISSN: 0300-483X; 1879-3185
• DOI: 10.1016/j.tox.2005.08.017

The protective effect of an antioxidant, Vitamin E ( dl-α-tocopherol, 100 mg/kg/day, 8 days p.o. in vivo and 10 and 50 μM in vitro) was tested against PCB-induced neurotoxicity. Microdialysis was used to investigate changes in the striatal extracellular dopamine level and in p-nNOS expression in PCB-treated (Aroclor 1254, 10 μg/ml, 2 μl/min, 5 h; 6 μg was infused by microdialysis probe) rats. Cell viability and levels of p-nNOS expression were observed in PCB-treated (Aroclor 1254, 5 μg/ml) immortalized dopaminergic cell line (CATH.a cells). Treatment with PCB: (1) decreased the extracellular dopamine level in rat striatum, (2) increased p-nNOS expression both in rat striatal tissue and in CATH.a cells, (3) reduced the cell viability of, and (4) increased LDH release by CATH.a cells. However, Vitamin E showed a protective effect against PCB-induced toxicity and downregulation of the extracellular dopamine level. These results indicate that Vitamin E may have neuroprotective effects by inhibiting PCB-induced nNOS phosphorylation.