Selection and Characterization of a DNA Aptamer Specifically Targeting Human HECT Ubiquitin Ligase WWP1

• Published in: International journal of molecular sciences Vol. 19; no. 3; p. 763
• Main Authors: Tucker, Wesley, Kinghorn, Andrew, Fraser, Lewis, Cheung, Yee-Wai, Tanner, Julian
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 07.03.2018; MDPI
• Subjects: Aptamers, Nucleotide - chemistry; Aptamers, Nucleotide - genetics; Aptamers, Nucleotide - metabolism; Base Sequence; Binding Sites; Calcification, Physiologic - genetics; Cell culture; Cell Line; Core Binding Factor Alpha 1 Subunit - metabolism; Deoxyribonucleic acid; DNA; Humans; Models, Molecular; Nucleic Acid Conformation; Osteoblasts - metabolism; Osteoporosis; Protein Binding; Protein Conformation; Protein Interaction Domains and Motifs; Protein Transport; Transcription factors; Ubiquitin-Protein Ligases - chemistry; Ubiquitin-Protein Ligases - metabolism; Ubiquitination; ubiquitin ligase; WWP1; targeted drug delivery; aptamer; SELEX

Nucleic acid aptamers hold promise as therapeutic tools for specific, tailored inhibition of protein targets with several advantages when compared to small molecules or antibodies. Nuclear WW domain containing E3 ubiquitin ligase 1 (WWP1) ubiquitin ligase poly-ubiquitinates Runt-related transcriptio...