Screening and Evaluation of New Hydroxymethylfurfural Oxidases for Furandicarboxylic Acid Production

• Published in: Applied and environmental microbiology Vol. 86; no. 16; p. 1
• Main Authors: Viñambres, Mario, Espada, Marta, Martínez, Angel T., Serrano, Ana
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 03.08.2020
• Subjects: Acid production; Alcohols; Aldehydes; Bacterial Proteins - metabolism; Biotechnology; Dicarboxylic Acids - metabolism; E coli; Enzymes; Escherichia coli - genetics; Escherichia coli - metabolism; Furaldehyde - analogs & derivatives; Furaldehyde - metabolism; Furans - metabolism; Gene expression; Genes; Hydrogen peroxide; Hydroxymethylfurfural; Methylophilaceae - genetics; Methylophilaceae - metabolism; Microorganisms, Genetically-Modified - genetics; Microorganisms, Genetically-Modified - metabolism; Oxidation; Oxidoreductases - metabolism; Pseudomonas - genetics; Pseudomonas - metabolism; Spotlight; enzyme stability; biotransformation; hydroxymethylfurfural; bioplastics; flavooxidases; Pseudomonas genes; database screening; furandicarboxylic acid; Escherichia coli expression; enzyme kinetics

HMFO is the only enzyme described to date that can catalyze by itself the three consecutive oxidation steps to produce FDCA from HMF. Unfortunately, only one HMFO enzyme is currently available for biotechnological application. This availability is enlarged here by the identification, heterologous pr...