Chaotropic salts: Novel modifiers for the capillary electrophoretic analysis of benzodiazepines
This paper describes a CE method for analyzing benzodiazepines using the chaotropic salts lithium trifluoromethanesulfonate (LiOTf), lithium hexafluorophosphate (LiPF6), and lithium bis(trifluoromethanesulfonyl)imide (LiNTf2) as modifiers in the running buffer. Although adequate resolution of seven...
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Published in | Electrophoresis Vol. 29; no. 16; pp. 3384 - 3390 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
WILEY-VCH Verlag
01.08.2008
WILEY‐VCH Verlag |
Subjects | |
Online Access | Get full text |
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Summary: | This paper describes a CE method for analyzing benzodiazepines using the chaotropic salts lithium trifluoromethanesulfonate (LiOTf), lithium hexafluorophosphate (LiPF6), and lithium bis(trifluoromethanesulfonyl)imide (LiNTf2) as modifiers in the running buffer. Although adequate resolution of seven benzodiazepine analytes occurred under the influence of each of the chaotropic anions, the separation efficiency was highest when bis(trifluoromethanesulfonyl)imide (Tf2N−) was the modifier. We applied affinity CE in conjunction with linear analysis to determine the association constants for the formation of complexes between the Tf2N− anion and the benzodiazepines. According to the estimated Gibbs free energies, the interactions between this chaotropic anion and the benzodiazepines were either ion–dipole or ion–induced dipole interactions. Adding chaotropic salts as modifiers into CE buffers is a simple and reproducible technique for separating benzodiazepines. |
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Bibliography: | ark:/67375/WNG-7L7P7TN6-H ArticleID:ELPS200800073 istex:03FE4136BF2F65F8DA0F9E35207A15D6D99EFAFE National Science Council, Taiwan, ROC - No. NSC 95-2113-M-009-033-MY3 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0173-0835 1522-2683 |
DOI: | 10.1002/elps.200800073 |