Glypican‐1 as a target for fluorescence molecular imaging of bladder cancer

• Published in: International journal of urology Vol. 28; no. 12; pp. 1290 - 1297
• Main Authors: Polikarpov, Dmitry M, Campbell, Douglas H, Zaslavsky, Alexander B, Lund, Maria E, Wu, Angela, Lu, Yanling, Palapattu, Ganesh S, Walsh, Bradley J, Zvyagin, Andrei V, Gillatt, David A
• Format: Journal Article
• Language: English
• Published: Australia Wiley Subscription Services, Inc 01.12.2021
• Subjects: Animals; Bladder cancer; Cancer; Carcinoma, Transitional Cell; Cell Line, Tumor; Flow cytometry; Fluorescence; fluorescence‐guided surgery; Glypicans; glypican‐1; Heparan sulfate proteoglycans; Immunoglobulin G; Intravenous administration; Mice; Mice, Nude; Molecular Imaging; monoclonal antibodies; Optical Imaging; Tissue Distribution; Tumors; urinary bladder neoplasms; Urinary Bladder Neoplasms - diagnostic imaging; Urothelial carcinoma; Xenografts; fluorescence-guided surgery; urinary bladder neoplasms; molecular imaging; glypican-1; monoclonal antibodies

Objectives To investigate whether anti‐glypican‐1 antibody Miltuximab conjugated with near‐infrared dye IRDye800CW can be used for in vivo fluorescence imaging of urothelial carcinoma. Methods The conjugate, Miltuximab‐IRDye800CW, was produced and characterized by size exclusion chromatography and f...