In vitro organogenesis from internodal segments of adult sweet orange plants

The objective of this work was to optimize in vitro plant regeneration via organogenesis from tissues of adult 'Hamlin', 'Pêra', and 'Valência' sweet orange plants. Explants were grown in EME culture medium with different concentrations of 6-benzylaminopurine (BAP) and...

Full description

Saved in:
Bibliographic Details
Published inPesquisa agropecuaria brasileira Vol. 46; no. 6; pp. 672 - 674
Main Authors Bassan, Meire Menezes, Mourão Filho, Francisco de Assis Alves, Miyata, Luzia Yuriko, Mendes, Beatriz Madalena Januzzi
Format Journal Article
LanguageEnglish
Portuguese
Published Embrapa Secretaria de Pesquisa e Desenvolvimento; Pesquisa Agropecuária Brasileira 01.06.2011
Embrapa Informação Tecnológica
Subjects
AGRICULTURE, DAIRY & ANIMAL SCIENCE
AGRICULTURE, MULTIDISCIPLINARY
biotecnologia
Citrus sinensis
gemas adventícias
reguladores vegetais
gemas adventícias
biotechnology
plant growth regulators
Citrus sinensis
biotecnologia
adventitious buds
reguladores vegetais
Online AccessGet full text

Cover

Abstract The objective of this work was to optimize in vitro plant regeneration via organogenesis from tissues of adult 'Hamlin', 'Pêra', and 'Valência' sweet orange plants. Explants were grown in EME culture medium with different concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA), at 27ºC in the absence of light for 50 days, followed by a 16-hour photoperiod for 20 days. Regeneration was assessed 50 and 70 days after in vitro culture. Organogenesis in cultivars Hamlin and Valência was promoted by EME supplemented with BAP, while NAA showed no apparent effect. O objetivo deste trabalho foi otimizar a regeneração in vitro via organogênese a partir de tecidos de plantas adultas das laranjas doces 'Hamlin', 'Pêra' e 'Valência'. Os explantes foram cultivados em meio EME com diferentes concentrações de 6-benzilaminopurina (BAP) e ácido naftalenoacético (ANA), a 27ºC sem luz por 50 dias, seguido de fotoperíodo de 16 horas por 20 dias. A regeneração foi avaliada 50 e 70 dias após o cultivo in vitro. A organogênese nas cultivares Hamlin e Valência foi favorecida pelo uso de EME suplementado com BAP, enquanto ANA não apresentou efeito aparente.
AbstractList The objective of this work was to optimize in vitro plant regeneration via organogenesis from tissues of adult 'Hamlin', 'Pêra', and 'Valência' sweet orange plants. Explants were grown in EME culture medium with different concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA), at 27ºC in the absence of light for 50 days, followed by a 16-hour photoperiod for 20 days. Regeneration was assessed 50 and 70 days after in vitro culture. Organogenesis in cultivars Hamlin and Valência was promoted by EME supplemented with BAP, while NAA showed no apparent effect. O objetivo deste trabalho foi otimizar a regeneração in vitro via organogênese a partir de tecidos de plantas adultas das laranjas doces 'Hamlin', 'Pêra' e 'Valência'. Os explantes foram cultivados em meio EME com diferentes concentrações de 6-benzilaminopurina (BAP) e ácido naftalenoacético (ANA), a 27ºC sem luz por 50 dias, seguido de fotoperíodo de 16 horas por 20 dias. A regeneração foi avaliada 50 e 70 dias após o cultivo in vitro. A organogênese nas cultivares Hamlin e Valência foi favorecida pelo uso de EME suplementado com BAP, enquanto ANA não apresentou efeito aparente.
The objective of this work was to optimize in vitro plant regeneration via organogenesis from tissues of adult 'Hamlin', 'Pêra', and 'Valência' sweet orange plants. Explants were grown in EME culture medium with different concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA), at 27ºC in the absence of light for 50 days, followed by a 16-hour photoperiod for 20 days. Regeneration was assessed 50 and 70 days after in vitro culture. Organogenesis in cultivars Hamlin and Valência was promoted by EME supplemented with BAP, while NAA showed no apparent effect.
Author Mendes, Beatriz Madalena Januzzi
Mourão Filho, Francisco de Assis Alves
Miyata, Luzia Yuriko
Bassan, Meire Menezes
AuthorAffiliation Universidade de São Paulo
AuthorAffiliation_xml – name: Universidade de São Paulo
Author_xml – sequence: 1
  givenname: Meire Menezes
  surname: Bassan
  fullname: Bassan, Meire Menezes
  organization: Universidade de São Paulo, Brazil
– sequence: 2
  givenname: Francisco de Assis Alves
  surname: Mourão Filho
  fullname: Mourão Filho, Francisco de Assis Alves
  organization: Universidade de São Paulo, Brazil
– sequence: 3
  givenname: Luzia Yuriko
  surname: Miyata
  fullname: Miyata, Luzia Yuriko
  organization: Universidade de São Paulo, Brazil
– sequence: 4
  givenname: Beatriz Madalena Januzzi
  surname: Mendes
  fullname: Mendes, Beatriz Madalena Januzzi
  organization: USP, Brazil
BookMark eNp1UV1LAzEQDFLBtvobzB-4muSSy92jFD8KBR9U8C3kks1x5ZqU5Kr4742tFEF8WHbZYYbZ2Rma-OABoWtKFlQ05OaZUEIKRvgbIzSPpMpFxRmanoDJr_kCzVLaEMJkWckpWq88fu_HGHCInfahAw-pT9jFsMW9HyH6YPWAE3Rb8GPCwWFt98OI0wfAmFnad4B3g87gJTp3ekhw9dPn6PX-7mX5WKyfHlbL23VhOKVjYWoGDSuNcZwJCS2YVkgq67qFEsoWGilZ40zDXWOM1o5xcLzSUtpWuErSco5WR10b9EbtYr_V8VMF3avDIl-idBx7M4CqhS2FqGpCpOCWC11XuuK0pLZsLbM6ay2OWsn0MAS1Cfvos3l1yFX9yTUT5JFgYkgpgjsZoER9f-Rf5hdbe36T
CitedBy_id crossref_primary_10_1007_s11627_020_10111_x
crossref_primary_10_3390_plants11141811
crossref_primary_10_1007_s11627_012_9476_6
crossref_primary_10_1016_j_hpj_2022_10_003
crossref_primary_10_1007_s11627_015_9743_4
crossref_primary_10_1007_s11627_019_09976_4
crossref_primary_10_1007_s11627_017_9866_x
crossref_primary_10_1016_j_scienta_2016_11_033
crossref_primary_10_1590_0100_29452019116
Cites_doi 10.1023/A:1008855922283
10.1590/S0100-204X2010000700004
10.1093/treephys/28.1.55
10.1270/jsbbs1951.40.199
10.1006/anbo.2000.1001
10.1590/S0100-29452005000300034
10.1080/14620316.2000.11511195
10.1016/S0168-9452(02)00401-6
10.1007/s002990050311
10.1023/A:1023375526781
ContentType Journal Article
Copyright This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright_xml – notice: This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DBID AAYXX
CITATION
GPN
DOA
DOI 10.1590/S0100-204X2011000600015
DatabaseName CrossRef
SciELO
Open Access: DOAJ - Directory of Open Access Journals
DatabaseTitle CrossRef
DatabaseTitleList CrossRef
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
DeliveryMethod fulltext_linktorsrc
Discipline Agriculture
DocumentTitleAlternate Organogênese in vitro a partir de segmentos internodais derivados de plantas adultas de laranja doce
EISSN 0100-204X
EndPage 674
ExternalDocumentID oai_doaj_org_article_85d3556800754d45a86a64131d3bd2da
S0100_204X2011000600015
10_1590_S0100_204X2011000600015
GroupedDBID 123
29O
2WC
5VS
AAFWJ
AAYXX
ABXHO
ADBBV
AENEX
AFPKN
ALMA_UNASSIGNED_HOLDINGS
APOWU
AZFZN
BCNDV
C1A
CITATION
E3Z
GROUPED_DOAJ
IPNFZ
KQ8
M~E
OK1
P2P
RIG
RNS
RSC
SCD
XSB
GPN
ID FETCH-LOGICAL-c411t-c82e923ccf4257ebecb571788be3e3be97729fc94f9ccaaf24ef46a77db5f6713
IEDL.DBID DOA
ISSN 0100-204X
1678-3921
IngestDate Tue Oct 22 15:07:27 EDT 2024
Fri Oct 18 20:36:14 EDT 2024
Fri Aug 23 00:58:06 EDT 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 6
Keywords gemas adventícias
biotechnology
plant growth regulators
Citrus sinensis
biotecnologia
adventitious buds
reguladores vegetais
Language English
Portuguese
License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. http://creativecommons.org/licenses/by-nc/4.0
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c411t-c82e923ccf4257ebecb571788be3e3be97729fc94f9ccaaf24ef46a77db5f6713
OpenAccessLink https://doaj.org/article/85d3556800754d45a86a64131d3bd2da
PageCount 3
ParticipantIDs doaj_primary_oai_doaj_org_article_85d3556800754d45a86a64131d3bd2da
scielo_journals_S0100_204X2011000600015
crossref_primary_10_1590_S0100_204X2011000600015
PublicationCentury 2000
PublicationDate 2011-06-00
20110601
2011-06-01
PublicationDateYYYYMMDD 2011-06-01
PublicationDate_xml – month: 06
  year: 2011
  text: 2011-06-00
PublicationDecade 2010
PublicationTitle Pesquisa agropecuaria brasileira
PublicationTitleAlternate Pesq. agropec. bras
PublicationYear 2011
Publisher Embrapa Secretaria de Pesquisa e Desenvolvimento; Pesquisa Agropecuária Brasileira
Embrapa Informação Tecnológica
Publisher_xml – name: Embrapa Secretaria de Pesquisa e Desenvolvimento; Pesquisa Agropecuária Brasileira
– name: Embrapa Informação Tecnológica
References OLIVEIRA M.L.P. de (ref9) 2010; 45
CERVERA M. (ref3) 2008; 28
PEÑA L. (ref10) 1997; 16
HIDAKA T. (ref6) 1990; 40
SILVA R.P. da (ref11) 2005; 27
MOREIRA-DIAS J.M. (ref8) 2000; 85
CERVERA M. (ref2) 1998; 7
CERVERA M. (ref4) 2000; 75
ALMEIDA W.A.B. (ref1) 2003; 164
GROSSER J.W. (ref5) 1990; 8
KOBAYASHI A.K. (ref7) 2003; 74
OLIVEIRA, M.L.P. de; COSTA, M.G.C.; SILVA, C.V. da; OTONI, W.C. 2010; 45
SILVA, R.P. da; SOUZA, E. dos S.; REBOUÇAS, F.S.; ALMEIDA, W.A.B. de 2005; 27
CERVERA, M.; NAVARRO, A.; NAVARRO, L.; PEÑA, L. 2008; 28
KOBAYASHI, A.K.; BESPALHOK, J.C.; PEREIRA, L.F.P.; VIEIRA, L.G.E. 2003; 74
CERVERA, M.; ORTEGA, C.; NAVARRO, A.; NAVARRO, L.; PEÑA, L. 2000; 75
MOREIRA-DIAS, J.M.; MOLINA, R.V.; BORDÓN, Y.; GUARDIOLA, J.L.; GARCÍA-LUIS, A. 2000; 85
HIDAKA, T.; OMURA, M.; UGAKI, M.; TOMIYAMA, M.; KATO, A.; OHSHIMA, M.; MOTOYOSHI, F. 1990; 40
PEÑA, L.; CERVERA, M.; JUÁREZ, J.; NAVARRO, A.; PINA, J.A.; NAVARRO, L. 1997; 16
GROSSER, J.W.; GMITTER JUNIOR, F.G. 1990; 8
ALMEIDA, W.A.B.; MOURÃO FILHO, F.A.A.; PINO, L.E.; BOSCARIOL, L.; RODRIGUEZ, A.P.M.; MENDES, B.M.J. 2003; 164
CERVERA, M.; JUÁREZ, J.; NAVARRO, A.; PINA, J.A.; DURAN-VILA, N.; NAVARRO, L.; PEÑA, L. 1998; 7
References_xml – volume: 7
  start-page: 51
  issn: 0962-8819
  year: 1998
  ident: ref2
  article-title: Genetic transformation and regeneration of mature tissues of woody fruit plants bypassing the juvenile stage
  publication-title: Transgenic Research
  doi: 10.1023/A:1008855922283
  contributor:
    fullname: CERVERA M.
– volume: 45
  start-page: 654
  issn: 0100-204X
  year: 2010
  ident: ref9
  article-title: Growth regulators, culture media and antibiotics in the in vitro shoot regeneration from mature tissue of citrus cultivars
  publication-title: Pesquisa Agropecuária Brasileira
  doi: 10.1590/S0100-204X2010000700004
  contributor:
    fullname: OLIVEIRA M.L.P. de
– volume: 28
  start-page: 55
  year: 2008
  ident: ref3
  article-title: Production of transgenic adult plants from clementine mandarin by enhancing cell competence for transformation and regeneration
  publication-title: Tree Physiology
  doi: 10.1093/treephys/28.1.55
  contributor:
    fullname: CERVERA M.
– volume: 40
  start-page: 199
  year: 1990
  ident: ref6
  article-title: Agrobacterium-mediated transformation and regeneration of Citrus spp. from suspension cells
  publication-title: Japanese Journal of Breeding
  doi: 10.1270/jsbbs1951.40.199
  contributor:
    fullname: HIDAKA T.
– volume: 85
  start-page: 103
  issn: 0305-7364
  year: 2000
  ident: ref8
  article-title: Direct and indirect shoot organogenic pathways in epicotyl cuttings of Troyer citrange differ in hormone requirements and in their response to light
  publication-title: Annals of Botany
  doi: 10.1006/anbo.2000.1001
  contributor:
    fullname: MOREIRA-DIAS J.M.
– volume: 8
  start-page: 339
  year: 1990
  ident: ref5
  article-title: Protoplast fusion and citrus improvement
  publication-title: Plant Breeding Reviews
  contributor:
    fullname: GROSSER J.W.
– volume: 27
  start-page: 484
  issn: 0100-2945
  year: 2005
  ident: ref11
  article-title: Otimização de protocolos para regeneração de plantas in vitro de tangerina 'Cleópatra' (Citrus reshni Hort. ex Tan.)
  publication-title: Revista Brasileira de Fruticultura
  doi: 10.1590/S0100-29452005000300034
  contributor:
    fullname: SILVA R.P. da
– volume: 75
  start-page: 26
  year: 2000
  ident: ref4
  article-title: Generation of transgenic citrus plants with the tolerance-to-salinity gene HAL2 from yeast
  publication-title: Journal of Horticultural Science and Biotechnology
  doi: 10.1080/14620316.2000.11511195
  contributor:
    fullname: CERVERA M.
– volume: 164
  start-page: 203
  issn: 0168-9452
  year: 2003
  ident: ref1
  article-title: Genetic transformation and plant recovery from mature tissues of Citrus sinensis L. Osbeck
  publication-title: Plant Science
  doi: 10.1016/S0168-9452(02)00401-6
  contributor:
    fullname: ALMEIDA W.A.B.
– volume: 16
  start-page: 731
  issn: 0721-7714
  year: 1997
  ident: ref10
  article-title: Genetic transformation of lime (Citrus aurantifolia): factors affecting transformation and regeneration
  publication-title: Plant Cell Reports
  doi: 10.1007/s002990050311
  contributor:
    fullname: PEÑA L.
– volume: 74
  start-page: 99
  issn: 0167-6857
  year: 2003
  ident: ref7
  article-title: Plant regeneration of sweet orange (Citrus sinensis) from thin sections of mature stem segments
  publication-title: Plant Cell, Tissue and Organ Culture
  doi: 10.1023/A:1023375526781
  contributor:
    fullname: KOBAYASHI A.K.
– volume: 75
  start-page: 26
  year: 2000
  end-page: 30
  article-title: Generation of transgenic citrus plants with the tolerance-to-salinity gene HAL2 from yeast
  publication-title: Journal of Horticultural Science and Biotechnology
  contributor:
    fullname: CERVERA, M.; ORTEGA, C.; NAVARRO, A.; NAVARRO, L.; PEÑA, L.
– volume: 27
  start-page: 484
  year: 2005
  end-page: 487
  article-title: Otimização de protocolos para regeneração de plantas in vitro de tangerina 'Cleópatra' (Citrus reshni Hort. ex Tan.)
  publication-title: Revista Brasileira de Fruticultura
  contributor:
    fullname: SILVA, R.P. da; SOUZA, E. dos S.; REBOUÇAS, F.S.; ALMEIDA, W.A.B. de
– volume: 74
  start-page: 99
  year: 2003
  end-page: 102
  article-title: Plant regeneration of sweet orange (Citrus sinensis) from thin sections of mature stem segments
  publication-title: Plant Cell, Tissue and Organ Culture
  contributor:
    fullname: KOBAYASHI, A.K.; BESPALHOK, J.C.; PEREIRA, L.F.P.; VIEIRA, L.G.E.
– volume: 45
  start-page: 654
  year: 2010
  end-page: 660
  article-title: Growth regulators, culture media and antibiotics in the in vitro shoot regeneration from mature tissue of citrus cultivars
  publication-title: Pesquisa Agropecuária Brasileira
  contributor:
    fullname: OLIVEIRA, M.L.P. de; COSTA, M.G.C.; SILVA, C.V. da; OTONI, W.C.
– volume: 28
  start-page: 55
  year: 2008
  end-page: 66
  article-title: Production of transgenic adult plants from clementine mandarin by enhancing cell competence for transformation and regeneration
  publication-title: Tree Physiology
  contributor:
    fullname: CERVERA, M.; NAVARRO, A.; NAVARRO, L.; PEÑA, L.
– volume: 8
  start-page: 339
  year: 1990
  end-page: 374
  article-title: Protoplast fusion and citrus improvement
  publication-title: Plant Breeding Reviews
  contributor:
    fullname: GROSSER, J.W.; GMITTER JUNIOR, F.G.
– volume: 40
  start-page: 199
  year: 1990
  end-page: 207
  article-title: Agrobacterium-mediated transformation and regeneration of Citrus spp. from suspension cells
  publication-title: Japanese Journal of Breeding
  contributor:
    fullname: HIDAKA, T.; OMURA, M.; UGAKI, M.; TOMIYAMA, M.; KATO, A.; OHSHIMA, M.; MOTOYOSHI, F.
– volume: 7
  start-page: 51
  year: 1998
  end-page: 59
  article-title: Genetic transformation and regeneration of mature tissues of woody fruit plants bypassing the juvenile stage
  publication-title: Transgenic Research
  contributor:
    fullname: CERVERA, M.; JUÁREZ, J.; NAVARRO, A.; PINA, J.A.; DURAN-VILA, N.; NAVARRO, L.; PEÑA, L.
– volume: 85
  start-page: 103
  year: 2000
  end-page: 110
  article-title: Direct and indirect shoot organogenic pathways in epicotyl cuttings of Troyer citrange differ in hormone requirements and in their response to light
  publication-title: Annals of Botany
  contributor:
    fullname: MOREIRA-DIAS, J.M.; MOLINA, R.V.; BORDÓN, Y.; GUARDIOLA, J.L.; GARCÍA-LUIS, A.
– volume: 164
  start-page: 203
  year: 2003
  end-page: 211
  article-title: Genetic transformation and plant recovery from mature tissues of Citrus sinensis L. Osbeck
  publication-title: Plant Science
  contributor:
    fullname: ALMEIDA, W.A.B.; MOURÃO FILHO, F.A.A.; PINO, L.E.; BOSCARIOL, L.; RODRIGUEZ, A.P.M.; MENDES, B.M.J.
– volume: 16
  start-page: 731
  year: 1997
  end-page: 737
  article-title: Genetic transformation of lime (Citrus aurantifolia): factors affecting transformation and regeneration
  publication-title: Plant Cell Reports
  contributor:
    fullname: PEÑA, L.; CERVERA, M.; JUÁREZ, J.; NAVARRO, A.; PINA, J.A.; NAVARRO, L.
SSID ssj0027367
Score 1.9932297
Snippet The objective of this work was to optimize in vitro plant regeneration via organogenesis from tissues of adult 'Hamlin', 'Pêra', and 'Valência' sweet orange...
SourceID doaj
scielo
crossref
SourceType Open Website
Open Access Repository
Aggregation Database
StartPage 672
SubjectTerms AGRICULTURE, DAIRY & ANIMAL SCIENCE
AGRICULTURE, MULTIDISCIPLINARY
biotecnologia
Citrus sinensis
gemas adventícias
reguladores vegetais
Title In vitro organogenesis from internodal segments of adult sweet orange plants
URI http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000600015&lng=en&tlng=en
https://doaj.org/article/85d3556800754d45a86a64131d3bd2da
Volume 46
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1LS8QwEA6yJz2IT1xf5CB4KrtJ00eOqyiriBdd2FtpkumysLTLturfdybtyoqIF6-hbco3SWa-ZPINY1cgpbYWSU5uUxUocCIwiYsD7Ry269Tl1mdbPMfjiXqcRtONUl-UE9bKA7fADdLIhaSSRb5NORXlaZzHuPIKFxonXRsaiWhNptZUK_S1Y0VMh_9aii6zK9LDwQsykCEODjX1vo8ESShw-OaXvHw_iYfi_FpsOpz7PbbbRYp81P7hPtuC8oDtjGarTi0DDtnTQ8nf582q4r44UzWjhWtec7ozwud-r69y-I0aZv4mG68K7gU3eP0B0OBbdLWALxeUDHPEJvd3r7fjoCuPEFglRBPYVAKGZ9YWNO_IGCZCcpamBkIIDWgKnAurVYGo53khFRQqzpPEmaiIkZwes15ZlXDCuLAOiZezoQKprBRGhxY9OdITsKFNRJ8N1-Bky1YFIyP2gHhmHs_sB559dkMgfj1OMta-AQHJOuNmfxm3z65bE2TdzKp_6-70P7o7Y9vtljFtspyzXrN6gwuMORpz6YfXJ8kezRo
link.rule.ids 230,315,786,790,870,891,2115,27955,27956
linkProvider Directory of Open Access Journals
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=In+vitro+organogenesis+from+internodal+segments+of+adult+sweet+orange+plants&rft.jtitle=Pesquisa+agropecuaria+brasileira&rft.au=Bassan%2C+Meire+Menezes&rft.au=Mour%C3%A3o+Filho%2C+Francisco+de+Assis+Alves&rft.au=Miyata%2C+Luzia+Yuriko&rft.au=Mendes%2C+Beatriz+Madalena+Januzzi&rft.date=2011-06-01&rft.issn=0100-204X&rft.eissn=0100-204X&rft.volume=46&rft.issue=6&rft.spage=672&rft.epage=674&rft_id=info:doi/10.1590%2FS0100-204X2011000600015&rft.externalDBID=n%2Fa&rft.externalDocID=10_1590_S0100_204X2011000600015
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0100-204X&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0100-204X&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0100-204X&client=summon