Differential Requirement for Proliferating Cell Nuclear Antigen in 5′ and 3′ Nick-directed Excision in Human Mismatch Repair

• Published in: The Journal of biological chemistry Vol. 279; no. 17; pp. 16912 - 16917
• Main Authors: Guo, Shuangli, Presnell, Steven R., Yuan, Fenghua, Zhang, Yanbin, Gu, Liya, Li, Guo-Min
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 23.04.2004
• Subjects: Adenosine Triphosphatases - physiology; Bacterial Proteins - physiology; Base Pair Mismatch; Base Sequence; Blotting, Southern; Cell Nucleus - metabolism; Cyclin-Dependent Kinase Inhibitor p21; Cyclins - metabolism; DNA - metabolism; DNA Repair; DNA, Complementary - metabolism; DNA-Binding Proteins - physiology; Dose-Response Relationship, Drug; Escherichia coli - metabolism; Escherichia coli Proteins; Glutathione Transferase - metabolism; HeLa Cells; Humans; Molecular Sequence Data; MutS DNA Mismatch-Binding Protein; Proliferating Cell Nuclear Antigen - metabolism; Proliferating Cell Nuclear Antigen - physiology; Protein Structure, Tertiary; Recombinant Fusion Proteins - metabolism

Proliferating cell nuclear antigen (PCNA) is involved in mammalian mismatch repair at a step prior to or at mismatch excision, but the molecular mechanism of this process is not fully understood. To examine the role of PCNA in mismatch-provoked and nick-directed excision, orientation-specific mismat...