FPF1 transgene leads to altered flowering time and root development in rice

• Published in: Plant cell reports Vol. 24; no. 2; pp. 79 - 85
• Main Authors: Xu, M.L, Jiang, J.F, Ge, L, Xu, Y.Y, Chen, H, Zhao, Y, Bi, Y.R, Wen, J.Q, Chong, K
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.05.2005; Springer Nature B.V
• Subjects: Arabidopsis; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Biological and medical sciences; Biotechnology; flowering; flowering promoting factor 1; Flowers & plants; Flowers - genetics; Flowers - growth & development; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Plant - genetics; Genetic engineering; Genetic technics; genetic transformation; Genetic Vectors - genetics; Genome, Plant; Glucuronidase - metabolism; Growth Inhibitors - genetics; Growth Inhibitors - metabolism; Methods. Procedures. Technologies; molecular sequence data; nucleotide sequences; Oryza - genetics; Oryza - growth & development; Oryza sativa; plant proteins; Plant Roots - genetics; Plant Roots - growth & development; Plants, Genetically Modified - genetics; Plants, Genetically Modified - growth & development; Promoter Regions, Genetic - genetics; rice; Time Factors; Transformation, Genetic - genetics; transgenes; Transgenic animals and transgenic plants; Transgenic plants; Oryza sativa; Monocotyledones; Root; Gramineae; AtFPF1; Angiospermae; Development; Flowering; Spermatophyta; Transgenic plant; Root development; Rice
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s00299-004-0906-8

AtFPF1 (FLOWERING PROMOTING FACTOR 1) is a gene that promotes flowering in Arabidopsis. An expression vector containing AtFPF1 driven by a Ubi-1 promoter was constructed. The gene was introduced into rice callus by Agrobacterium-mediated transformation and fertile plants were obtained. The presence of AtFPF1 in rice plants was confirmed by PCR, Southern and Northern blot analyses, as well as by beta-glucuronidase assay. The results showed that, as in Arabidopsis, AtFPF1 reduced flowering time in rice. Furthermore, introduction of AtFPF1 enhanced adventitious root formation but inhibited root growth in rice during the seedling stage. The results suggest that AtFPF1 promotes flowering time in both dicots and monocots, and plays a role in the initiation of adventitious roots in rice.