Functional domain analysis of SOX18 transcription factor using a single-chain variable fragment-based approach

• Published in: mAbs Vol. 10; no. 4; pp. 596 - 606
• Main Authors: Fontaine, Frank R, Goodall, Stephen, Prokop, Jeremy W, Howard, Christopher B, Moustaqil, Mehdi, Kumble, Sumukh, Rasicci, Daniel T, Osborne, Geoffrey W, Gambin, Yann, Sierecki, Emma, Jones, Martina L, Zuegg, Johannes, Mahler, Stephen, Francois, Mathias
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 19.05.2018
• Subjects: Humans; Single-Chain Antibodies; SOXF Transcription Factors - analysis; SOXF Transcription Factors - chemistry; protein-protein interaction; antibody; SOX18 transcription factor; scFv; transcriptional activation

Antibodies are routinely used to study the activity of transcription factors, using various in vitro and in vivo approaches such as electrophoretic mobility shift assay, enzyme-linked immunosorbent assay, genome-wide method analysis coupled with next generation sequencing, or mass spectrometry. More...