Shp2 in myocytes is essential for cardiovascular and neointima development

• Published in: Journal of molecular and cellular cardiology Vol. 137; pp. 71 - 81
• Main Authors: Gong, Hui, Ni, Jiaojiao, Xu, Zhiyong, Huang, Jiaqi, Zhang, Jie, Huang, Yizhou, Zeng, Chunlai, Zhang, Xue, Cheng, Hongqiang, Ke, Yuehai
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2019
• Subjects: Extracellular matrix; Neointima; Proliferation; Shp2; Vascular smooth muscle cells; AA; cKO; Lu; Proliferation; LCA; iKO; M; TA; ECM; Shp2; RCA; Neointima; TUNEL; PAH; Extracellular matrix; TGF-β; NI; VSMC; Vascular smooth muscle cells; PDGF-BB
• ISSN: 0022-2828; 1095-8584; 1095-8584
• DOI: 10.1016/j.yjmcc.2019.09.014

Mutations in the PTPN11 gene, which encodes the protein tyrosine phosphatase Shp2, cause Noonan syndrome and LEOPARD syndrome, inherited multifaceted diseases including cardiac and vascular defects. However, the function of Shp2 in blood vessels, especially in vascular smooth muscle cells (VSMCs), remains largely unknown. We generated mice in which Shp2 was specifically deleted in VSMCs and embryonic cardiomyocytes using the SM22α-Cre transgenic mouse line. Conditional Shp2 knockout resulted in massive hemorrhage, cardiovascular defects and embryonic lethality at the late embryonic developmental stage (embryonic date 16.5). The thinning of artery walls in Shp2-knockout embryos was due to decreased VSMC number and reduced extracellular matrix deposition. Myocyte proliferation was decreased in Shp2-knockout arteries and hearts. Importantly, cardiomyocyte-specific Shp2-knockout did not cause similar vascular defects. Shp2 was required for TGFβ1-induced expression of ECM components, including collagens in VSMCs. In addition, collagens were sufficient to promote Shp2-inefficient VSMC proliferation. Finally, Shp2 was deleted in adult mouse VSMCs by using SMMHC-CreERT2 and tamoxifen induction. Shp2 deletion dramatically inhibited the expression of ECM components, proliferation of VSMCs and neointima formation in a carotid artery ligation model. Therefore, Shp2 is required for myocyte proliferation in cardiovascular development and vascular remodeling through TGFβ1-regulated collagen synthesis. •Shp2 deletion in mouse myocytes leads to cardiovascular defects and embryonic death.•Shp2 is essential for ECM production and cell proliferation in vascular development and remodeling.•Shp2 regulates TGFβ1-Smad2 signaling in VSMCs.