ZnO nanowire-based fluorometric enzymatic assays for lactate and cholesterol

• Published in: Mikrochimica acta (1966) Vol. 187; no. 3; p. 180
• Main Authors: Briones, María, Busó-Rogero, Carlos, Catalán-Gómez, Sergio, García-Mendiola, Tania, Pariente, Félix, Redondo-Cubero, Andrés, Lorenzo, María Encarnación
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.03.2020; Springer
• Subjects: Analytical Chemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Cholesterol; Hydrogen peroxide; IX NyNA 2019. International Congress on Analytical Nanoscience and Nanotechnology; Lactates; Microengineering; Nanochemistry; Nanotechnology; Original Paper; Oxidases; Type 2 diabetes; Zinc oxide; Lactate oxidase; Indirect optical method; Hydrogen peroxide; ZnO NWs-enzyme bioconjugate; Photoluminescence; Fluorescence; Cholesterol oxidase; Collisional quenching mechanism; Human serum sample; Enzymatic reaction

A rapid fluorometric method is described for the determination of lactate and cholesterol by using ZnO nanowires (ZnO NWs). The assay is based on the detection of the hydrogen peroxide generated during the enzymatic reactions of the oxidation of lactate or cholesterol. Taking advantage of the electr...