Modeling bispecific monoclonal antibody interaction with two cell membrane targets indicates the importance of surface diffusion

• Published in: mAbs Vol. 8; no. 5; pp. 905 - 915
• Main Authors: Sengers, Bram G., McGinty, Sean, Nouri, Fatma Z., Argungu, Maryam, Hawkins, Emma, Hadji, Aymen, Weber, Andrew, Taylor, Adam, Sepp, Armin
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 03.07.2016
• Subjects: Animals; Antibodies, Bispecific - immunology; Antibodies, Monoclonal - immunology; Antibody Affinity - immunology; Diffusion; Humans; Models, Theoretical; Monte Carlo Method; avidity; therapeutic; diffusion; modeling; Monte Carlo; simulation; bispecific antibody; specificity; Affinity

We have developed a mathematical framework for describing a bispecific monoclonal antibody interaction with two independent membrane-bound targets that are expressed on the same cell surface. The bispecific antibody in solution binds either of the two targets first, and then cross-links with the sec...