Human cells enter mitosis with damaged DNA after treatment with pharmacological concentrations of genotoxic agents

• Published in: Biochemical journal Vol. 446; no. 3; p. 373
• Main Authors: Kubara, Philip M, Kernéis-Golsteyn, Sophie, Studény, Aurélie, Lanser, Brittany B, Meijer, Laurent, Golsteyn, Roy M
• Format: Journal Article
• Language: English
• Published: England 15.09.2012
• Subjects: CDC2 Protein Kinase - metabolism; Cell Cycle; Cell Cycle Proteins - metabolism; Checkpoint Kinase 1; DNA - metabolism; DNA Damage - physiology; Genome; HT29 Cells; Humans; Mitosis; Mutagens - adverse effects; Mutagens - pharmacology; Phosphorylation; Polo-Like Kinase 1; Protein Kinases - metabolism; Protein Serine-Threonine Kinases - metabolism; Proto-Oncogene Proteins - metabolism

In the present paper, we report that mitosis is a key step in the cellular response to genotoxic agents in human cells. Cells with damaged DNA recruit γH2AX (phosphorylated histone H2AX), phosphorylate Chk1 (checkpoint kinase 1) and arrest in the G2-phase of the cell cycle. Strikingly, nearly all ce...