Protease Inhibitors of Chickpea (Cicer arietinumL) During Seed Development

• Published in: Journal of the science of food and agriculture Vol. 74; no. 4; pp. 509 - 512
• Main Authors: Harsulkar, Abhay M, Giri, Ashok P, Kothekar, Vijay S
• Format: Journal Article
• Language: English
• Published: London John Wiley & Sons, Ltd 01.08.1997; Wiley; Published for the Society of Chemical Industry by Elsevier Applied Science
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; chickpea; developing seeds; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Hydrolases; post-translational modification; trypsin inhibitors; Seeds; Serine endopeptidases; Enzyme; Enzyme inhibitor; Peptidases; Chymotrypsin; Grain legume; Trypsin; Leguminosae; Cicer arietinum; Dicotyledones; Angiospermae; Development; Hydrolases; Spermatophyta; Comparative study; Cultivar
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/(SICI)1097-0010(199708)74:4<509::AID-JSFA827>3.0.CO;2-2

Developing seeds of two chickpea varieties were collected on the 20th, 40th and 60th day after flowering (DAF) and analysed for trypsin inhibitors (TI). The varieties differed in their TI profiles and activity units. The TI band and activity were not detectable at 20 DAF in the varieties Annigeri and BDN 9‐3. Annigeri showed the highest total and specific TI activity, and five isoinhibitors at 40 DAF, which decreased to four at 60 DAF. The appearance and disappearance of TI bands during seed development indicate their specific post‐translational modification and/or the presence of isolated gene groups. © 1997 SCI.