Engineering Tools for the Development of Recombinant Lactic Acid Bacteria

Lactic acid bacteria (LAB) is mainly used in food fermentation. In addition, LAB fermentation technology has been studied in the development of industrial food additives, nutrients, or enzymes used in food processing. In the field of red biotechnology, LAB is approved and is generally recognized as...

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Bibliographic Details
Published inBiotechnology journal Vol. 15; no. 6; p. e1900344
Main Authors Cho, Sung Won, Yim, Jaewoo, Seo, Sang Woo
Format Journal Article
LanguageEnglish
Published Germany 01.06.2020
Subjects
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biotechnology - methods
DNA Replication
Fermentation
Food Microbiology
Genetic Engineering - methods
Lactic Acid - metabolism
Lactobacillales - genetics
Lactobacillales - metabolism
Plasmids
Transformation, Genetic
synthetic biology
lactic acid bacteria
genetic engineering
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Summary:Lactic acid bacteria (LAB) is mainly used in food fermentation. In addition, LAB fermentation technology has been studied in the development of industrial food additives, nutrients, or enzymes used in food processing. In the field of red biotechnology, LAB is approved and is generally recognized as a safe organism and is considered safe for biotherapeutic treatments. Recent clinical trials have demonstrated the medicinal value of therapeutic recombinant LAB and the suitability of innate mechanisms of secretion and anchoring for therapeutic applications such as antibody or vaccine production. However, the gram-positive phenotypic trait of LAB creates challenges for genetic modifications when compared to other conventional workhorse bacteria, resulting in exclusive developments of genetic tools for engineering LAB. In this review, several distinct approaches in gene expression for engineering LAB are discussed.
ISSN:1860-7314
DOI:10.1002/biot.201900344