Functional inactivation of p73, a homolog of p53 tumor suppressor protein, by human papillomavirus E6 proteins
Human papillomavirus (HPV) is strongly implicated as a causative agent in the etiology of cervical cancer. Of its gene products, E6 binds to and inactivates p53 tumor suppressor protein by ubiquitin/proteasome‐dependent degradation. Recently, p73, a novel family of p53, has been identified and demon...
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Published in | International journal of cancer Vol. 91; no. 6; pp. 822 - 827 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
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New York
John Wiley & Sons, Inc
15.03.2001
Wiley-Liss |
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Abstract | Human papillomavirus (HPV) is strongly implicated as a causative agent in the etiology of cervical cancer. Of its gene products, E6 binds to and inactivates p53 tumor suppressor protein by ubiquitin/proteasome‐dependent degradation. Recently, p73, a novel family of p53, has been identified and demonstrated, like p53, to activate p21WAF1. Here we show that p73 is also inactivated by HPV‐E6, but ubiquitin‐mediated proteolysis is not responsive. Yeast two‐hybrid and GST pull‐down assays indicate a physical interaction between p73 and either HPV‐16 or HPV‐11 E6 proteins in vivo and in vitro, respectively. The transactivation domain (amino acid residues 1 to 49) is found to be absolutely required for the interaction. Transient co‐expression of E6 significantly inhibits the p73‐mdiated activation of p21WAF1 promoter in a p53‐defective C33A cell line. Using Gal4‐p73 fusion protein, we demonstrate that E6 inhibition of p73 transactivation function is independent of sequence‐specific DNA binding, which is confirmed by a direct electrophoretic mobility shift assay. Moreover, E6 inhibits p73 function by interfering with the activity of the amino‐terminal activation domain. Co‐transfection of E6 mutants reveals that the same portion of E6 appears to be responsible for the inactivation of p53 and p73 function. However, the inactivation mechanism of p73 is clearly different from that of p53, because p73, unlike p53, is inactivated by both high‐ and low‐risk E6s and is not susceptible to E6‐dependent proteolysis. These overall results, consequently, suggest that in addition to the inactivation of p53, the functional interference of p73 by HPV‐E6 may, at least in part, contribute to E6‐mediated transformation and hyperproliferation of cervical cells. © 2001 Wiley‐Liss, Inc. |
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AbstractList | Human papillomavirus (HPV) is strongly implicated as a causative agent in the etiology of cervical cancer. Of its gene products, E6 binds to and inactivates p53 tumor suppressor protein by ubiquitin/proteasome-dependent degradation. Recently, p73, a novel family of p53, has been identified and demonstrated, like p53, to activate p21 super(WAF1). Here we show that p73 is also inactivated by HPV-E6, but ubiquitin-mediated proteolysis is not responsive. Yeast two-hybrid and GST pull-down assays indicate a physical interaction between p73 and either HPV-16 or HPV-11 E6 proteins in vivo and in vitro, respectively. The transactivation domain (amino acid residues 1 to 49) is found to be absolutely required for the interaction. Transient co-expression of E6 significantly inhibits the p73-mediated activation of p21 super(WAF1) promoter in a p53-defective C33A cell line. Using Gal4-p73 fusion protein, we demonstrate that E6 inhibition of p73 transactivation function is independent of sequence-specific DNA binding, which is confirmed by a direct electrophoretic mobility shift assay. Moreover, E6 inhibits p73 function by interfering with the activity of the amino-terminal activation domain. Co-transfection of E6 mutants reveals that the same portion of E6 appears to be responsible for the inactivation of p53 and p73 function. However, the inactivation mechanism of p73 is clearly different from that of p53, because p73, unlike p53, is inactivated by both high- and low-risk E6s and is not susceptible to E6-dependent proteolysis. These overall results, consequently, suggest that in addition to the inactivation of p53, the functional interference of p73 by HPV-E6 may, at least in part, contribute to E6-mediated transformation and hyperproliferation of cervical cells. Human papillomavirus (HPV) is strongly implicated as a causative agent in the etiology of cervical cancer. Of its gene products, E6 binds to and inactivates p53 tumor suppressor protein by ubiquitin/proteasome‐dependent degradation. Recently, p73, a novel family of p53, has been identified and demonstrated, like p53, to activate p21WAF1. Here we show that p73 is also inactivated by HPV‐E6, but ubiquitin‐mediated proteolysis is not responsive. Yeast two‐hybrid and GST pull‐down assays indicate a physical interaction between p73 and either HPV‐16 or HPV‐11 E6 proteins in vivo and in vitro, respectively. The transactivation domain (amino acid residues 1 to 49) is found to be absolutely required for the interaction. Transient co‐expression of E6 significantly inhibits the p73‐mdiated activation of p21WAF1 promoter in a p53‐defective C33A cell line. Using Gal4‐p73 fusion protein, we demonstrate that E6 inhibition of p73 transactivation function is independent of sequence‐specific DNA binding, which is confirmed by a direct electrophoretic mobility shift assay. Moreover, E6 inhibits p73 function by interfering with the activity of the amino‐terminal activation domain. Co‐transfection of E6 mutants reveals that the same portion of E6 appears to be responsible for the inactivation of p53 and p73 function. However, the inactivation mechanism of p73 is clearly different from that of p53, because p73, unlike p53, is inactivated by both high‐ and low‐risk E6s and is not susceptible to E6‐dependent proteolysis. These overall results, consequently, suggest that in addition to the inactivation of p53, the functional interference of p73 by HPV‐E6 may, at least in part, contribute to E6‐mediated transformation and hyperproliferation of cervical cells. © 2001 Wiley‐Liss, Inc. Human papillomavirus (HPV) is strongly implicated as a causative agent in the etiology of cervical cancer. Of its gene products, E6 binds to and inactivates p53 tumor suppressor protein by ubiquitin/proteasome-dependent degradation. Recently, p73, a novel family of p53, has been identified and demonstrated, like p53, to activate p21(WAF1). Here we show that p73 is also inactivated by HPV-E6, but ubiquitin-mediated proteolysis is not responsive. Yeast two-hybrid and GST pull-down assays indicate a physical interaction between p73 and either HPV-16 or HPV-11 E6 proteins in vivo and in vitro, respectively. The transactivation domain (amino acid residues 1 to 49) is found to be absolutely required for the interaction. Transient co-expression of E6 significantly inhibits the p73-mdiated activation of p21(WAF1) promoter in a p53-defective C33A cell line. Using Gal4-p73 fusion protein, we demonstrate that E6 inhibition of p73 transactivation function is independent of sequence-specific DNA binding, which is confirmed by a direct electrophoretic mobility shift assay. Moreover, E6 inhibits p73 function by interfering with the activity of the amino-terminal activation domain. Co-transfection of E6 mutants reveals that the same portion of E6 appears to be responsible for the inactivation of p53 and p73 function. However, the inactivation mechanism of p73 is clearly different from that of p53, because p73, unlike p53, is inactivated by both high- and low-risk E6s and is not susceptible to E6-dependent proteolysis. These overall results, consequently, suggest that in addition to the inactivation of p53, the functional interference of p73 by HPV-E6 may, at least in part, contribute to E6-mediated transformation and hyperproliferation of cervical cells. |
Author | Kim, Eun‐Joo Lee, Jung‐Young Park, Jong‐Sup Sin, Hong‐Sig Um, Soo‐Jong Namkoong, Sung‐Eun |
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Keywords | Human Homology Papovaviridae Uterine cervix Malignant tumor Inactivation Carcinogenesis Female genital diseases Infection Papillomavirus Virus Human papillomavirus TP53 Gene Viral disease Genetics Uterine cervix diseases Tumor suppressor gene |
Language | English |
License | CC BY 4.0 Copyright 2001 Wiley-Liss, Inc. |
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SubjectTerms | AE6 protein Ap73 protein Apoptosis Biological and medical sciences Blotting, Western cervical cancer Cyclin-Dependent Kinase Inhibitor p21 Cyclins - metabolism DNA - metabolism DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism E6 protein Electrophoresis, Agar Gel Enzyme-Linked Immunosorbent Assay Female genital diseases Genes, Tumor Suppressor - physiology Glutathione Transferase - chemistry Gynecology. Andrology. Obstetrics HPV Human papillomavirus 11 human papillomavirus 16 Humans Medical sciences Mutation Nuclear Proteins - genetics Nuclear Proteins - metabolism Oncogene Proteins, Viral - genetics Oncogene Proteins, Viral - metabolism p53 p73 p73 protein Papillomaviridae - physiology Protein Binding Recombinant Fusion Proteins - metabolism Transfection Tumor Cells, Cultured Tumor Protein p73 Tumor Suppressor Protein p53 - genetics Tumor Suppressor Protein p53 - metabolism Tumor Suppressor Proteins Tumor Virus Infections - virology Tumors Two-Hybrid System Techniques Waf1 gene |
Title | Functional inactivation of p73, a homolog of p53 tumor suppressor protein, by human papillomavirus E6 proteins |
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