Effect of poly(amidoamine) dendrimers on the structure and activity of immune molecules
Poly(amidoamine) (PAMAM) dendrimers are widely used biomedical polymers, which are extensively applied in drug delivery, gene delivery, contrast agent, etc. In these biomedical applications, the bio-safety of the PAMAM dendrimers is a critical issue, which affects not only their toxicity to the host...
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| Published in | Biochimica et biophysica acta Vol. 1850; no. 2; pp. 419 - 425 |
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| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
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Netherlands
01.02.2015
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| Abstract | Poly(amidoamine) (PAMAM) dendrimers are widely used biomedical polymers, which are extensively applied in drug delivery, gene delivery, contrast agent, etc. In these biomedical applications, the bio-safety of the PAMAM dendrimers is a critical issue, which affects not only their toxicity to the host but also the expected in vivo biofunctions of the materials. To clarify the bio-safety of PAMAM dendrimers, the effects of generation 5 PAMAM dendrimers with amine, hydroxyl or carboxyl groups on immune molecules were explored in this work.
Specifically, the effect of the PAMAM dendrimers on the secondary structure and conformation of immune molecule γ-globulin was studied by using ultraviolet-visible, fluorescence, and circular dichroism spectroscopies. The effect of the PAMAM dendrimers on complement activation was determined by enzyme-linked immunosorbent assay. Further, the effect of the PAMAM dendrimers on antigen-antibody reaction was studied by using human red blood cell agglutination assay.
The results showed that, the PAMAM dendrimers could affect the secondary structure and conformation of γ-globulin, and inhibited complement activation. Generation 5 PAMAM dendrimer with carboxyl group at 10mg/mL impaired red blood cell (RBC) antigen-antibody reaction.
From these results, the effects of the PAMAM dendrimers on immune molecules depend on their bulk structure and surface groups.
This work provides important information for the immunocompatibility evaluation, preclinical design, and clinical applications of PAMAM dendrimers. |
|---|---|
| AbstractList | Poly(amidoamine) (PAMAM) dendrimers are widely used biomedical polymers, which are extensively applied in drug delivery, gene delivery, contrast agent, etc. In these biomedical applications, the bio-safety of the PAMAM dendrimers is a critical issue, which affects not only their toxicity to the host but also the expected in vivo biofunctions of the materials. To clarify the bio-safety of PAMAM dendrimers, the effects of generation 5 PAMAM dendrimers with amine, hydroxyl or carboxyl groups on immune molecules were explored in this work.BACKGROUNDPoly(amidoamine) (PAMAM) dendrimers are widely used biomedical polymers, which are extensively applied in drug delivery, gene delivery, contrast agent, etc. In these biomedical applications, the bio-safety of the PAMAM dendrimers is a critical issue, which affects not only their toxicity to the host but also the expected in vivo biofunctions of the materials. To clarify the bio-safety of PAMAM dendrimers, the effects of generation 5 PAMAM dendrimers with amine, hydroxyl or carboxyl groups on immune molecules were explored in this work.Specifically, the effect of the PAMAM dendrimers on the secondary structure and conformation of immune molecule γ-globulin was studied by using ultraviolet-visible, fluorescence, and circular dichroism spectroscopies. The effect of the PAMAM dendrimers on complement activation was determined by enzyme-linked immunosorbent assay. Further, the effect of the PAMAM dendrimers on antigen-antibody reaction was studied by using human red blood cell agglutination assay.METHODSSpecifically, the effect of the PAMAM dendrimers on the secondary structure and conformation of immune molecule γ-globulin was studied by using ultraviolet-visible, fluorescence, and circular dichroism spectroscopies. The effect of the PAMAM dendrimers on complement activation was determined by enzyme-linked immunosorbent assay. Further, the effect of the PAMAM dendrimers on antigen-antibody reaction was studied by using human red blood cell agglutination assay.The results showed that, the PAMAM dendrimers could affect the secondary structure and conformation of γ-globulin, and inhibited complement activation. Generation 5 PAMAM dendrimer with carboxyl group at 10mg/mL impaired red blood cell (RBC) antigen-antibody reaction.RESULTSThe results showed that, the PAMAM dendrimers could affect the secondary structure and conformation of γ-globulin, and inhibited complement activation. Generation 5 PAMAM dendrimer with carboxyl group at 10mg/mL impaired red blood cell (RBC) antigen-antibody reaction.From these results, the effects of the PAMAM dendrimers on immune molecules depend on their bulk structure and surface groups.CONCLUSIONSFrom these results, the effects of the PAMAM dendrimers on immune molecules depend on their bulk structure and surface groups.This work provides important information for the immunocompatibility evaluation, preclinical design, and clinical applications of PAMAM dendrimers.GENERAL SIGNIFICANCEThis work provides important information for the immunocompatibility evaluation, preclinical design, and clinical applications of PAMAM dendrimers. Poly(amidoamine) (PAMAM) dendrimers are widely used biomedical polymers, which are extensively applied in drug delivery, gene delivery, contrast agent, etc. In these biomedical applications, the bio-safety of the PAMAM dendrimers is a critical issue, which affects not only their toxicity to the host but also the expected in vivo biofunctions of the materials. To clarify the bio-safety of PAMAM dendrimers, the effects of generation 5 PAMAM dendrimers with amine, hydroxyl or carboxyl groups on immune molecules were explored in this work. Specifically, the effect of the PAMAM dendrimers on the secondary structure and conformation of immune molecule γ-globulin was studied by using ultraviolet-visible, fluorescence, and circular dichroism spectroscopies. The effect of the PAMAM dendrimers on complement activation was determined by enzyme-linked immunosorbent assay. Further, the effect of the PAMAM dendrimers on antigen-antibody reaction was studied by using human red blood cell agglutination assay. The results showed that, the PAMAM dendrimers could affect the secondary structure and conformation of γ-globulin, and inhibited complement activation. Generation 5 PAMAM dendrimer with carboxyl group at 10mg/mL impaired red blood cell (RBC) antigen-antibody reaction. From these results, the effects of the PAMAM dendrimers on immune molecules depend on their bulk structure and surface groups. This work provides important information for the immunocompatibility evaluation, preclinical design, and clinical applications of PAMAM dendrimers. Poly(amidoamine) (PAMAM) dendrimers are widely used biomedical polymers, which are extensively applied in drug delivery, gene delivery, contrast agent, etc. In these biomedical applications, the bio-safety of the PAMAM dendrimers is a critical issue, which affects not only their toxicity to the host but also the expected in vivo biofunctions of the materials. To clarify the bio-safety of PAMAM dendrimers, the effects of generation 5 PAMAM dendrimers with amine, hydroxyl or carboxyl groups on immune molecules were explored in this work.Specifically, the effect of the PAMAM dendrimers on the secondary structure and conformation of immune molecule γ-globulin was studied by using ultraviolet-visible, fluorescence, and circular dichroism spectroscopies. The effect of the PAMAM dendrimers on complement activation was determined by enzyme-linked immunosorbent assay. Further, the effect of the PAMAM dendrimers on antigen–antibody reaction was studied by using human red blood cell agglutination assay.The results showed that, the PAMAM dendrimers could affect the secondary structure and conformation of γ-globulin, and inhibited complement activation. Generation 5 PAMAM dendrimer with carboxyl group at 10mg/mL impaired red blood cell (RBC) antigen–antibody reaction.From these results, the effects of the PAMAM dendrimers on immune molecules depend on their bulk structure and surface groups.This work provides important information for the immunocompatibility evaluation, preclinical design, and clinical applications of PAMAM dendrimers. |
| Author | Yin, Jian Qiu, Xiaozhong Liu, Zonghua Lin, Jiansheng Zhang, Yi Li, Chenghua Xue, Wei Hua, Wenxi |
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| Cites_doi | 10.1016/S0304-4165(98)00048-8 10.1177/0883911514521921 10.1093/jmcb/mjp002 10.1016/j.bbapap.2007.04.016 10.2147/IJN.S36111 10.1016/j.nano.2006.04.002 10.1021/ar600012y 10.1096/fj.06-7380com 10.1016/S1570-9639(03)00117-1 10.1021/nn303472r 10.1016/j.bbrc.2006.04.041 10.1016/j.bioorg.2009.07.006 10.1016/S0142-9612(01)00043-6 10.1021/nn9011187 10.1021/am900891h 10.1021/mp2006054 10.1128/JVI.75.7.3453-3461.2001 10.1007/s11249-006-9185-6 10.1211/0022357055803 10.1021/bm0504882 10.1016/j.taap.2010.04.014 10.1016/j.jconrel.2012.02.005 10.1016/j.ijpharm.2010.05.030 |
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| References | Huang (10.1016/j.bbagen.2014.11.016_bb0010) 2007; 21 Mosqueira (10.1016/j.bbagen.2014.11.016_bb0120) 2001; 22 Li (10.1016/j.bbagen.2014.11.016_bb0035) 2009; 1 Nakashima (10.1016/j.bbagen.2014.11.016_bb0105) 2007; 26 Naha (10.1016/j.bbagen.2014.11.016_bb0045) 2010; 246 Vermeer (10.1016/j.bbagen.2014.11.016_bb0100) 1998; 1425 Shcharbin (10.1016/j.bbagen.2014.11.016_bb0075) 2007; 1774 Toda (10.1016/j.bbagen.2014.11.016_bb0110) 2010; 2 Jones (10.1016/j.bbagen.2014.11.016_bb0030) 2012; 6 Klajnert (10.1016/j.bbagen.2014.11.016_bb0065) 2006; 345 Fu (10.1016/j.bbagen.2014.11.016_bb0020) 2014; 29 Supattapone (10.1016/j.bbagen.2014.11.016_bb0070) 2001; 75 Kuo (10.1016/j.bbagen.2014.11.016_bb0040) 2005; 57 Leroueil (10.1016/j.bbagen.2014.11.016_bb0060) 2007; 40 Biricova (10.1016/j.bbagen.2014.11.016_bb0015) 2009; 37 Klajnert (10.1016/j.bbagen.2014.11.016_bb0080) 2003; 1648 Jones (10.1016/j.bbagen.2014.11.016_bb0025) 2012; 9 Kainthan (10.1016/j.bbagen.2014.11.016_bb0115) 2006; 7 Liu (10.1016/j.bbagen.2014.11.016_bb0085) 2012; 160 Gupta (10.1016/j.bbagen.2014.11.016_bb0005) 2006; 2 Lacerda (10.1016/j.bbagen.2014.11.016_bb0095) 2009; 4 Fröhlich (10.1016/j.bbagen.2014.11.016_bb0055) 2012; 7 Wang (10.1016/j.bbagen.2014.11.016_bb0050) 2010; 395 |
| References_xml | – volume: 1425 start-page: 1 issue: 1 year: 1998 ident: 10.1016/j.bbagen.2014.11.016_bb0100 article-title: Structural changes of IgG induced by heat treatment and by adsorption onto a hydrophobic Teflon surface studied by circular dichroism spectroscopy publication-title: Biochim. Biophys. Acta doi: 10.1016/S0304-4165(98)00048-8 – volume: 29 start-page: 165 issue: 2 year: 2014 ident: 10.1016/j.bbagen.2014.11.016_bb0020 article-title: Effects of poly(amidoamine) dendrimers on the structure and function of key blood components publication-title: J. Bioact. Compat. Polym. doi: 10.1177/0883911514521921 – volume: 1 start-page: 37 issue: 1 year: 2009 ident: 10.1016/j.bbagen.2014.11.016_bb0035 article-title: PAMAM nanoparticles promote acute lung injury by inducing autophagic cell death through the Akt–TSC2–mTOR signaling pathway publication-title: J. Mol. Cell Biol. doi: 10.1093/jmcb/mjp002 – volume: 1774 start-page: 946 issue: 7 year: 2007 ident: 10.1016/j.bbagen.2014.11.016_bb0075 article-title: Serum albumins have five sites for binding of cationic dendrimers publication-title: Biochim. Biophys. Acta doi: 10.1016/j.bbapap.2007.04.016 – volume: 7 start-page: 5577 year: 2012 ident: 10.1016/j.bbagen.2014.11.016_bb0055 article-title: The role of surface charge in cellular uptake and cytotoxicity of medical nanoparticles publication-title: Int. J. Nanomedicine doi: 10.2147/IJN.S36111 – volume: 2 start-page: 66 issue: 2 year: 2006 ident: 10.1016/j.bbagen.2014.11.016_bb0005 article-title: A review of in vitro–in vivo investigations on dendrimers: the novel nanoscopic drug carriers publication-title: Nanomed. Nanotechnol. Biol. Med. doi: 10.1016/j.nano.2006.04.002 – volume: 40 start-page: 335 issue: 5 year: 2007 ident: 10.1016/j.bbagen.2014.11.016_bb0060 article-title: Nanoparticle interaction with biological membranes: does nanotechnology present a Janus face? publication-title: Acc. Chem. Res. doi: 10.1021/ar600012y – volume: 21 start-page: 1117 issue: 4 year: 2007 ident: 10.1016/j.bbagen.2014.11.016_bb0010 article-title: Efficient gene delivery targeted to the brain using a transferrin-conjugated polyethyleneglycol-modified polyamidoamine dendrimer publication-title: FASEB J. doi: 10.1096/fj.06-7380com – volume: 1648 start-page: 115 issue: 1–2 year: 2003 ident: 10.1016/j.bbagen.2014.11.016_bb0080 article-title: Interactions between PAMAM dendrimers and bovine serum albumin publication-title: Biochim. Biophys. Acta doi: 10.1016/S1570-9639(03)00117-1 – volume: 6 start-page: 9900 issue: 11 year: 2012 ident: 10.1016/j.bbagen.2014.11.016_bb0030 article-title: Cationic PAMAM dendrimers aggressively initiate blood clot formation publication-title: ACS Nano doi: 10.1021/nn303472r – volume: 345 start-page: 21 issue: 1 year: 2006 ident: 10.1016/j.bbagen.2014.11.016_bb0065 article-title: Influence of dendrimer's structure on its activity against amyloid fibril formation publication-title: Biochem. Biophys. Res. Commun. doi: 10.1016/j.bbrc.2006.04.041 – volume: 37 start-page: 185 issue: 6 year: 2009 ident: 10.1016/j.bbagen.2014.11.016_bb0015 article-title: Dendrimers: analytical characterization and applications publication-title: Bioorg. Chem. doi: 10.1016/j.bioorg.2009.07.006 – volume: 22 start-page: 2967 issue: 22 year: 2001 ident: 10.1016/j.bbagen.2014.11.016_bb0120 article-title: Relationship between complement activation, cellular uptake and surface physicochemical aspects of novel PEG-modified nanocapsules publication-title: Biomaterials doi: 10.1016/S0142-9612(01)00043-6 – volume: 4 start-page: 365 issue: 1 year: 2009 ident: 10.1016/j.bbagen.2014.11.016_bb0095 article-title: Interaction of gold nanoparticles with common human blood proteins publication-title: ACS Nano doi: 10.1021/nn9011187 – volume: 2 start-page: 1107 issue: 4 year: 2010 ident: 10.1016/j.bbagen.2014.11.016_bb0110 article-title: Effects of hydrophobicity and electrostatic charge on complement activation by amino groups publication-title: ACS Appl. Mater. Interfaces doi: 10.1021/am900891h – volume: 9 start-page: 1599 issue: 6 year: 2012 ident: 10.1016/j.bbagen.2014.11.016_bb0025 article-title: Cationic PAMAM dendrimers disrupt key platelet functions publication-title: Mol. Pharm. doi: 10.1021/mp2006054 – volume: 75 start-page: 3453 issue: 7 year: 2001 ident: 10.1016/j.bbagen.2014.11.016_bb0070 article-title: Branched polyamines cure prion-infected neuroblastoma cells publication-title: J. Virol. doi: 10.1128/JVI.75.7.3453-3461.2001 – volume: 26 start-page: 145 issue: 2 year: 2007 ident: 10.1016/j.bbagen.2014.11.016_bb0105 article-title: Influence of protein conformation on frictional properties of poly(vinyl alcohol) hydrogel for artificial cartilage publication-title: Tribol. Lett. doi: 10.1007/s11249-006-9185-6 – volume: 57 start-page: 489 issue: 4 year: 2005 ident: 10.1016/j.bbagen.2014.11.016_bb0040 article-title: Mechanism of cell death induced by cationic dendrimers in RAW 264.7 murine macrophage-like cells publication-title: J. Pharm. Pharmacol. doi: 10.1211/0022357055803 – volume: 7 start-page: 703 issue: 3 year: 2006 ident: 10.1016/j.bbagen.2014.11.016_bb0115 article-title: Biocompatibility testing of branched and linear polyglycidol publication-title: Biomacromolecules doi: 10.1021/bm0504882 – volume: 246 start-page: 91 issue: 1 year: 2010 ident: 10.1016/j.bbagen.2014.11.016_bb0045 article-title: Reactive oxygen species (ROS) induced cytokine production and cytotoxicity of PAMAM dendrimers in J774A.1 cells publication-title: Toxicol. Appl. Pharmacol. doi: 10.1016/j.taap.2010.04.014 – volume: 160 start-page: 14 issue: 1 year: 2012 ident: 10.1016/j.bbagen.2014.11.016_bb0085 article-title: Interactions between solubilized polymer molecules and blood components publication-title: J. Control. Release doi: 10.1016/j.jconrel.2012.02.005 – volume: 395 start-page: 298 issue: 1 year: 2010 ident: 10.1016/j.bbagen.2014.11.016_bb0050 article-title: Inhibition of bacterial growth and intramniotic infection in a guinea pig model of chorioamnionitis using PAMAM dendrimers publication-title: Int. J. Pharm. doi: 10.1016/j.ijpharm.2010.05.030 |
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| SubjectTerms | agglutination Antigen-Antibody Reactions circular dichroism spectroscopy complement Dendrimers - chemistry drugs enzyme-linked immunosorbent assay erythrocytes Erythrocytes - chemistry Erythrocytes - immunology Female fluorescence gamma-globulins gamma-Globulins - chemistry gamma-Globulins - immunology gene transfer Humans Male polymers Structure-Activity Relationship toxicity |
| Title | Effect of poly(amidoamine) dendrimers on the structure and activity of immune molecules |
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