Development and evaluation of a sandwich ELISA method for the detection of human CD306

• Published in: Journal of immunological methods Vol. 396; no. 1-2; pp. 65 - 73
• Main Authors: Xie, Xin, Wang, ChunYan, Xie, YuanQi, Wang, Xin, Chen, GuangSheng, Yan, XingRong, Cui, JiHong, Chen, FuLin, Li, Hongmin, Jin, BoQuan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 31.10.2013
• Subjects: Adolescent; Adult; Aged; Antibodies, Monoclonal - immunology; Biomarkers - blood; Biomarkers - urine; CD305; CD306; Cloning, Molecular; complementary DNA; disease diagnosis; DNA, Complementary - genetics; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Female; fever; heart diseases; Hemorrhagic Fever with Renal Syndrome - blood; Hemorrhagic Fever with Renal Syndrome - immunology; Hemorrhagic Fever with Renal Syndrome - urine; Humans; immunoglobulins; Immunosuppression; Inflammation; Leukocytes, Mononuclear - immunology; Male; Middle Aged; monoclonal antibodies; Monoclonal antibody; mononuclear leukocytes; Neoplasms - blood; Neoplasms - immunology; Neoplasms - urine; patients; Pregnancy; pregnant women; protein synthesis; Receptors, Immunologic - analysis; Receptors, Immunologic - blood; Receptors, Immunologic - genetics; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - immunology; Rheumatic Heart Disease - blood; Rheumatic Heart Disease - immunology; Rheumatic Heart Disease - urine; Sandwich ELISA; secretion; urine; Young Adult; Immunosuppression; mAbs; CD306; TMB; CD305; Monoclonal antibody; LAIR; PBMCs; HFRS; ABTS; Sandwich ELISA; peripheral blood mononuclear cells; 3,3′,5,5′-tetramethylbenzidine; hemorrhagic fever with renal syndrome; leukocyte-associated Ig-like receptor; 2,2′-azino-bis-[3-ethylbenzthiazoline-6-sulfonic acid]; monoclonal antibodies

CD306, also known as soluble leukocyte-associated immunoglobulin-like receptor-2 (LAIR-2), is a member of an immunoglobulin superfamily with the shared characteristic of an immunoglobulin-like C2-type domain. CD306 is speculated to be secretory and has 84% similarity with the extracellular domain of...