IL-10 induces TGF-β secretion, TGF-β receptor II upregulation, and IgA secretion in B cells

• Published in: European cytokine network Vol. 30; no. 3; pp. 107 - 113
• Main Authors: Bagheri, Yasser, Babaha, Fateme, Falak, Reza, Yazdani, Reza, Azizi, Gholamreza, Sadri, Maryam, Abolhassani, Hassan, Shekarabi, Mehdi, Aghamohammadi, Asghar
• Format: Journal Article
• Language: English
• Published: France Springer Nature B.V 01.09.2019
• Subjects: B-Lymphocytes - immunology; CD40 antigen; CD40 Antigens - immunology; CD40 Ligand - immunology; Class switching; Enzyme-linked immunosorbent assay; Flow cytometry; Humans; Immunoglobulin A; Immunoglobulin A - immunology; Immunoglobulin Class Switching - immunology; Interleukin 10; Interleukin-10 - immunology; Lymphocyte Activation - immunology; Lymphocytes B; Plasma cells; Receptors, Transforming Growth Factor beta - immunology; Transforming Growth Factor beta - immunology; Transforming growth factor-b; Up-Regulation - immunology; class switching; immunoglobulin A; transforming growth factor-β; interleukin-10
• ISSN: 1148-5493; 1952-4005
• DOI: 10.1684/ecn.2019.0434

Interleukin-10 (IL-10) is a pleiotropic cytokine, which has both regulatory and stimulatory effects on different immune cell types. Different studies have reported the importance of IL-10 and Transforming growth factor-beta (TGF-β) in the regulation of B cell class switching the production of immunoglobulin A (IgA); however, the underlying mechanisms remain to be fully elucidated. The objective of this study was to investigate the TGF-β response during B stimulation of human B cells by IL-10. Pan B cells of healthy donors were negatively purified by a magnetic cell separation technique. B cells were cultured with multimeric CD40 ligand (mCD40L) and IL-10 for two and seven days. After harvesting in specific days, TGF-β receptor II and surface IgA expression was determined by flow cytometry, while IgA and TGF-β secretion was assessed by enzyme-linked immunosorbent assay. B cells endogenously expressed TGF-β receptor II and after 48 hours cultivation with mCD40L or mCD40L plus IL-10, both the expression of this receptor and the production of TGF-β were significantly increased. Notably, TGF-β levels following stimulation with mCD40L and IL-10 were higher than those produced by B cells stimulated with mCD40L alone. Furthermore, at day 7 and following IL-10 stimulation, there was a significant rise in the amount of IgA secretion by class-switched plasma cells, which was higher than stimulation with mCD40L alone. Our findings suggest that IL-10 can modulate TGF-β production and TGF-β receptor expression in mCD40-activated human B lymphocytes.