Ethanol increases the paracellular permeability of monolayers of CAPAN-1 pancreatic duct cells

• Published in: Journal of molecular histology Vol. 35; no. 4; pp. 355 - 362
• Main Authors: Rotoli, B M, Orlandini, G, Guizzardi, S, Uggeri, J, Dall'Asta, V, Gazzola, G C, Bussolati, O, Gatti, R
• Format: Journal Article
• Language: English
• Published: Netherlands Springer Nature B.V 01.05.2004
• Subjects: Actins - metabolism; Carcinoma, Pancreatic Ductal - metabolism; Carcinoma, Pancreatic Ductal - pathology; Carcinoma, Pancreatic Ductal - ultrastructure; Cell Line, Tumor; Ethanol - pharmacology; Humans; Immunohistochemistry; Mannitol - metabolism; Membrane Proteins - metabolism; Microscopy, Electron, Transmission; Occludin; Pancreas; Pancreatic Neoplasms - metabolism; Pancreatic Neoplasms - pathology; Pancreatic Neoplasms - ultrastructure; Permeability; Permeability - drug effects; Phosphoproteins - metabolism; Proteins; Solvents - pharmacology; Tight Junctions - drug effects; Tight Junctions - metabolism; Zonula Occludens-1 Protein

When grown on permeable supports, pancreatic duct adenocarcinoma CAPAN-1 cells establish very high values of transepithelial resistance (TER). The addition of ethanol produced a dose-related, reversible drop in the TER of these cells, ranging from 15% (with 1% ethanol) to 65% (with 10% ethanol). The...