Global Mapping of Protein–Lipid Interactions by Using Modified Choline‐Containing Phospholipids Metabolically Synthesized in Live Cells

• Published in: Angewandte Chemie International Edition Vol. 56; no. 21; pp. 5829 - 5833
• Main Authors: Wang, Danyang, Du, Shubo, Cazenave‐Gassiot, Amaury, Ge, Jingyan, Lee, Jun‐Seok, Wenk, Markus R., Yao, Shao Q.
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 15.05.2017
• Edition: International ed. in English
• Subjects: Alkynes; Choline; click chemistry; Fatty acids; Incompatibility; Lipids; Mammalian cells; Metabolism; Peptide mapping; phosphatidylcholine; Phospholipids; photo-crosslinking; Proteins; protein–lipid interactions; Proteomics; quantitative proteomics; Synthesis; click chemistry; photo-crosslinking; protein-lipid interactions; quantitative proteomics; phosphatidylcholine

The protein–lipid interaction is an essential metabolic process that mediates cellular signaling and functions. Existing strategies for large‐scale mapping studies of the protein–lipid interaction fall short in their incompatibility with metabolic incorporation or inability to remove unwanted interf...