Global Mapping of Protein–Lipid Interactions by Using Modified Choline‐Containing Phospholipids Metabolically Synthesized in Live Cells

The protein–lipid interaction is an essential metabolic process that mediates cellular signaling and functions. Existing strategies for large‐scale mapping studies of the protein–lipid interaction fall short in their incompatibility with metabolic incorporation or inability to remove unwanted interf...

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Published inAngewandte Chemie International Edition Vol. 56; no. 21; pp. 5829 - 5833
Main Authors Wang, Danyang, Du, Shubo, Cazenave‐Gassiot, Amaury, Ge, Jingyan, Lee, Jun‐Seok, Wenk, Markus R., Yao, Shao Q.
Format Journal Article
LanguageEnglish
Published Germany Wiley Subscription Services, Inc 15.05.2017
EditionInternational ed. in English
Subjects
Alkynes
Choline
click chemistry
Fatty acids
Incompatibility
Lipids
Mammalian cells
Metabolism
Peptide mapping
phosphatidylcholine
Phospholipids
photo-crosslinking
Proteins
protein–lipid interactions
Proteomics
quantitative proteomics
Synthesis
click chemistry
photo-crosslinking
protein-lipid interactions
quantitative proteomics
phosphatidylcholine
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Abstract The protein–lipid interaction is an essential metabolic process that mediates cellular signaling and functions. Existing strategies for large‐scale mapping studies of the protein–lipid interaction fall short in their incompatibility with metabolic incorporation or inability to remove unwanted interferences from lipidated proteins. By incorporating an alkyne‐containing choline head group and a diazirine‐modified fatty acid simultaneously into choline‐containing phospholipids synthesized from live mammalian cells, protein–phospholipid interactions have been successfully imaged in live cells. Subsequent in situ profiling of the modified Cho phospholipid‐crosslinked proteins followed by quantitative proteomics allowed identification of several hundred putative phospholipid‐interacting proteins, some of which were further validated. Double the load: A novel double incorporation strategy for metabolic biosynthesis of bifunctional choline‐containing phospholipids (for example phosphatidylcholine (PC); see picture) was developed. This strategy offers significant improvement for global mapping of genuine protein–lipid interactions from live mammalian cells.
AbstractList The protein-lipid interaction is an essential metabolic process that mediates cellular signaling and functions. Existing strategies for large-scale mapping studies of the protein-lipid interaction fall short in their incompatibility with metabolic incorporation or inability to remove unwanted interferences from lipidated proteins. By incorporating an alkyne-containing choline head group and a diazirine-modified fatty acid simultaneously into choline-containing phospholipids synthesized from live mammalian cells, protein-phospholipid interactions have been successfully imaged in live cells. Subsequent insitu profiling of the modified Cho phospholipid-crosslinked proteins followed by quantitative proteomics allowed identification of several hundred putative phospholipid-interacting proteins, some of which were further validated.
The protein-lipid interaction is an essential metabolic process that mediates cellular signaling and functions. Existing strategies for large-scale mapping studies of the protein-lipid interaction fall short in their incompatibility with metabolic incorporation or inability to remove unwanted interferences from lipidated proteins. By incorporating an alkyne-containing choline head group and a diazirine-modified fatty acid simultaneously into choline-containing phospholipids synthesized from live mammalian cells, protein-phospholipid interactions have been successfully imaged in live cells. Subsequent in situ profiling of the modified Cho phospholipid-crosslinked proteins followed by quantitative proteomics allowed identification of several hundred putative phospholipid-interacting proteins, some of which were further validated.
The protein-lipid interaction is an essential metabolic process that mediates cellular signaling and functions. Existing strategies for large-scale mapping studies of the protein-lipid interaction fall short in their incompatibility with metabolic incorporation or inability to remove unwanted interferences from lipidated proteins. By incorporating an alkyne-containing choline head group and a diazirine-modified fatty acid simultaneously into choline-containing phospholipids synthesized from live mammalian cells, protein-phospholipid interactions have been successfully imaged in live cells. Subsequent in situ profiling of the modified Cho phospholipid-crosslinked proteins followed by quantitative proteomics allowed identification of several hundred putative phospholipid-interacting proteins, some of which were further validated.The protein-lipid interaction is an essential metabolic process that mediates cellular signaling and functions. Existing strategies for large-scale mapping studies of the protein-lipid interaction fall short in their incompatibility with metabolic incorporation or inability to remove unwanted interferences from lipidated proteins. By incorporating an alkyne-containing choline head group and a diazirine-modified fatty acid simultaneously into choline-containing phospholipids synthesized from live mammalian cells, protein-phospholipid interactions have been successfully imaged in live cells. Subsequent in situ profiling of the modified Cho phospholipid-crosslinked proteins followed by quantitative proteomics allowed identification of several hundred putative phospholipid-interacting proteins, some of which were further validated.
The protein–lipid interaction is an essential metabolic process that mediates cellular signaling and functions. Existing strategies for large‐scale mapping studies of the protein–lipid interaction fall short in their incompatibility with metabolic incorporation or inability to remove unwanted interferences from lipidated proteins. By incorporating an alkyne‐containing choline head group and a diazirine‐modified fatty acid simultaneously into choline‐containing phospholipids synthesized from live mammalian cells, protein–phospholipid interactions have been successfully imaged in live cells. Subsequent in situ profiling of the modified Cho phospholipid‐crosslinked proteins followed by quantitative proteomics allowed identification of several hundred putative phospholipid‐interacting proteins, some of which were further validated. Double the load: A novel double incorporation strategy for metabolic biosynthesis of bifunctional choline‐containing phospholipids (for example phosphatidylcholine (PC); see picture) was developed. This strategy offers significant improvement for global mapping of genuine protein–lipid interactions from live mammalian cells.
Author Ge, Jingyan
Wenk, Markus R.
Lee, Jun‐Seok
Yao, Shao Q.
Wang, Danyang
Du, Shubo
Cazenave‐Gassiot, Amaury
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  organization: National University of Singapore
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Cites_doi 10.1016/j.cell.2015.05.045
10.1002/anie.201503814
10.1038/sj.embor.7400311
10.1002/ange.201102542
10.1038/nrm2934
10.1038/nmeth.1293
10.1038/nrm2335
10.1002/anie.201300683
10.1016/j.chembiol.2008.11.009
10.1038/71366
10.1021/ja502780z
10.1002/anie.201210178
10.1002/ange.201503814
10.1038/nrm3286
10.1038/nrm4080
10.1021/cr300419p
10.1038/nmeth.2368
10.1002/anie.201102542
10.1021/cr2001977
10.1073/pnas.0907864106
10.1016/j.cbpa.2014.07.015
10.1038/nchembio.1908
10.1016/S0021-9258(19)38742-3
10.1002/ange.201300683
10.1016/j.cbpa.2012.10.034
10.1074/jbc.M109.056960
10.1021/ja502109n
10.1002/ange.201210178
10.1016/j.cell.2010.11.033
10.1073/pnas.0912306107
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Keywords click chemistry
photo-crosslinking
protein-lipid interactions
quantitative proteomics
phosphatidylcholine
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References 2010; 11
2015; 161
2015; 16
2010; 107
2015; 11
2010; 143
2008; 9
2010; 285
2000; 2
2012; 13
1990; 265
2014; 136
2011; 111
2014; 21
2013 2013; 52 125
2013; 17
2015; 137
2013; 10
2015 2015; 54 127
2013; 113
2005; 6
2011 2011; 50 123
2009; 6
2009; 16
2009; 106
e_1_2_2_4_1
e_1_2_2_25_1
e_1_2_2_5_1
e_1_2_2_24_1
e_1_2_2_6_1
e_1_2_2_22_2
e_1_2_2_23_1
e_1_2_2_7_1
e_1_2_2_21_2
e_1_2_2_1_1
e_1_2_2_20_1
e_1_2_2_2_1
e_1_2_2_3_1
e_1_2_2_9_1
e_1_2_2_29_1
e_1_2_2_8_1
e_1_2_2_28_1
e_1_2_2_27_1
e_1_2_2_9_2
e_1_2_2_26_1
e_1_2_2_14_1
e_1_2_2_12_2
e_1_2_2_13_1
e_1_2_2_12_1
e_1_2_2_11_1
e_1_2_2_10_1
e_1_2_2_30_1
e_1_2_2_30_2
e_1_2_2_19_1
e_1_2_2_17_2
e_1_2_2_18_1
e_1_2_2_16_2
e_1_2_2_17_1
e_1_2_2_15_2
References_xml – volume: 10
  start-page: 259
  year: 2013
  end-page: 264
  publication-title: Nat. Methods
– volume: 52 125
  start-page: 4033 4125
  year: 2013 2013
  end-page: 4038 4130
  publication-title: Angew. Chem. Int. Ed. Angew. Chem.
– volume: 143
  start-page: 888
  year: 2010
  end-page: 895
  publication-title: Cell
– volume: 9
  start-page: 162
  year: 2008
  end-page: 176
  publication-title: Nat. Rev. Mol. Cell Biol.
– volume: 106
  start-page: 15332
  year: 2009
  end-page: 15337
  publication-title: Proc. Natl. Acad. Sci. USA
– volume: 6
  start-page: 135
  year: 2009
  end-page: 138
  publication-title: Nat. Methods
– volume: 11
  start-page: 593
  year: 2010
  end-page: 598
  publication-title: Nat. Rev. Mol. Cell Biol.
– volume: 17
  start-page: 90
  year: 2013
  end-page: 101
  publication-title: Curr. Opin. Chem. Biol.
– volume: 107
  start-page: 8627
  year: 2010
  end-page: 8632
  publication-title: Proc. Natl. Acad. Sci. USA
– volume: 136
  start-page: 9990
  year: 2014
  end-page: 9998
  publication-title: J. Am. Chem. Soc.
– volume: 137
  start-page: 556
  year: 2015
  end-page: 559
  publication-title: J. Am. Chem. Soc.
– volume: 16
  start-page: 753
  year: 2015
  end-page: 761
  publication-title: Nat. Rev. Mol. Cell Biol.
– volume: 285
  start-page: 7358
  year: 2010
  end-page: 7365
  publication-title: J. Biol. Chem.
– volume: 161
  start-page: 1668
  year: 2015
  publication-title: Cell
– volume: 2
  start-page: 42
  year: 2000
  end-page: 49
  publication-title: Nat. Cell Biol.
– volume: 50 123
  start-page: 6776 6908
  year: 2011 2011
  end-page: 6781 6913
  publication-title: Angew. Chem. Int. Ed. Angew. Chem.
– volume: 13
  start-page: 168
  year: 2012
  end-page: 182
  publication-title: Nat. Rev. Mol. Cell Biol.
– volume: 16
  start-page: 3
  year: 2009
  end-page: 14
  publication-title: Chem. Biol.
– volume: 6
  start-page: 28
  year: 2005
  end-page: 32
  publication-title: EMBO Rep.
– volume: 54 127
  start-page: 9679 9815
  year: 2015 2015
  end-page: 9682 9818
  publication-title: Angew. Chem. Int. Ed. Angew. Chem.
– volume: 21
  start-page: 144
  year: 2014
  end-page: 153
  publication-title: Curr. Opin. Chem. Biol.
– volume: 11
  start-page: 807
  year: 2015
  end-page: 814
  publication-title: Nat. Chem. Biol.
– volume: 113
  start-page: 7880
  year: 2013
  end-page: 7929
  publication-title: Chem. Rev.
– volume: 111
  start-page: 6341
  year: 2011
  end-page: 6385
  publication-title: Chem. Rev.
– volume: 265
  start-page: 9800
  year: 1990
  end-page: 9807
  publication-title: J. Biol. Chem.
– volume: 52 125
  start-page: 8551 8713
  year: 2013 2013
  end-page: 8556 8718
  publication-title: Angew. Chem. Int. Ed. Angew. Chem.
– ident: e_1_2_2_8_1
  doi: 10.1016/j.cell.2015.05.045
– ident: e_1_2_2_30_1
  doi: 10.1002/anie.201503814
– ident: e_1_2_2_28_1
  doi: 10.1038/sj.embor.7400311
– ident: e_1_2_2_17_2
  doi: 10.1002/ange.201102542
– ident: e_1_2_2_2_1
  doi: 10.1038/nrm2934
– ident: e_1_2_2_21_2
  doi: 10.1038/nmeth.1293
– ident: e_1_2_2_22_2
– ident: e_1_2_2_4_1
  doi: 10.1038/nrm2335
– ident: e_1_2_2_9_1
  doi: 10.1002/anie.201300683
– ident: e_1_2_2_13_1
  doi: 10.1016/j.chembiol.2008.11.009
– ident: e_1_2_2_6_1
  doi: 10.1038/71366
– ident: e_1_2_2_20_1
– ident: e_1_2_2_23_1
  doi: 10.1021/ja502780z
– ident: e_1_2_2_12_1
  doi: 10.1002/anie.201210178
– ident: e_1_2_2_26_1
– ident: e_1_2_2_30_2
  doi: 10.1002/ange.201503814
– ident: e_1_2_2_10_1
  doi: 10.1038/nrm3286
– ident: e_1_2_2_29_1
  doi: 10.1038/nrm4080
– ident: e_1_2_2_5_1
  doi: 10.1021/cr300419p
– ident: e_1_2_2_7_1
  doi: 10.1038/nmeth.2368
– ident: e_1_2_2_17_1
  doi: 10.1002/anie.201102542
– ident: e_1_2_2_16_2
  doi: 10.1021/cr2001977
– ident: e_1_2_2_19_1
  doi: 10.1073/pnas.0907864106
– ident: e_1_2_2_3_1
  doi: 10.1016/j.cbpa.2014.07.015
– ident: e_1_2_2_24_1
  doi: 10.1038/nchembio.1908
– ident: e_1_2_2_27_1
  doi: 10.1016/S0021-9258(19)38742-3
– ident: e_1_2_2_14_1
– ident: e_1_2_2_9_2
  doi: 10.1002/ange.201300683
– ident: e_1_2_2_11_1
  doi: 10.1016/j.cbpa.2012.10.034
– ident: e_1_2_2_25_1
  doi: 10.1074/jbc.M109.056960
– ident: e_1_2_2_15_2
  doi: 10.1021/ja502109n
– ident: e_1_2_2_12_2
  doi: 10.1002/ange.201210178
– ident: e_1_2_2_1_1
  doi: 10.1016/j.cell.2010.11.033
– ident: e_1_2_2_18_1
  doi: 10.1073/pnas.0912306107
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Snippet The protein–lipid interaction is an essential metabolic process that mediates cellular signaling and functions. Existing strategies for large‐scale mapping...
The protein-lipid interaction is an essential metabolic process that mediates cellular signaling and functions. Existing strategies for large-scale mapping...
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SubjectTerms Alkynes
Choline
click chemistry
Fatty acids
Incompatibility
Lipids
Mammalian cells
Metabolism
Peptide mapping
phosphatidylcholine
Phospholipids
photo-crosslinking
Proteins
protein–lipid interactions
Proteomics
quantitative proteomics
Synthesis
Title Global Mapping of Protein–Lipid Interactions by Using Modified Choline‐Containing Phospholipids Metabolically Synthesized in Live Cells
URI https://onlinelibrary.wiley.com/doi/abs/10.1002%2Fanie.201702509
https://www.ncbi.nlm.nih.gov/pubmed/28429463
https://www.proquest.com/docview/1896026497
https://www.proquest.com/docview/1891133232
Volume 56
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