A New One‐Pot Fluorescence Derivatization Strategy for Highly Sensitive MicroRNA Analysis

• Published in: Chemistry : a European journal Vol. 26; no. 25; pp. 5639 - 5647
• Main Authors: Pan, Li, Zhang, Huaisheng, Zhao, Jingjin, Ogungbe, Ifedayo Victor, Zhao, Shulin, Liu, Yi‐Ming
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 04.05.2020
• Subjects: Acetaldehyde - analogs & derivatives; Acetaldehyde - chemistry; Adenine; Adenine - chemistry; Apoptosis; Chemistry; Chloroacetaldehyde; Chromatography; Chromatography, High Pressure Liquid - methods; dyes/pigments; Fluorescence; Fluorescence spectroscopy; Gene expression; High performance liquid chromatography; Humans; Liquid chromatography; magnetic properties; Mass Spectrometry; Mass spectroscopy; MicroRNAs; MicroRNAs - analysis; miRNA; Nucleic acids; RNA recognition; Spectrometry, Fluorescence - methods; Strategy; synthetic methods; Water; dyes/pigments; RNA recognition; fluorescence spectroscopy; magnetic properties; synthetic methods

MicroRNAs (miRNAs) modulate the expression of over 30 % of mammalian genes during development and apoptosis, and abnormal expression of miRNAs may lead to a range of human pathologies. Therefore, analysis of miRNAs is valuable for disease diagnostics. In this work, a novel one‐pot fluorescence deriv...