Rapid and Selective Labeling of Endogenous Transmembrane Proteins in Living Cells with a Difluorophenyl Ester Affinity‐Based Probe
The long‐term stability of affinity‐based protein labeling probes is crucial to obtain reproducible protein labeling results. However, highly stable probes generally suffer from low protein labeling efficiency and pose significant challenges when labeling low abundance native proteins in living cell...
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Published in | Chemistry, an Asian journal Vol. 15; no. 21; pp. 3416 - 3420 |
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Language | English |
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Abstract | The long‐term stability of affinity‐based protein labeling probes is crucial to obtain reproducible protein labeling results. However, highly stable probes generally suffer from low protein labeling efficiency and pose significant challenges when labeling low abundance native proteins in living cells. In this paper, we report that protein labeling probes based on an ortho‐difluorophenyl ester reactive module exhibit long‐term stability in DMSO stock solution and aqueous buffer, yet they can undergo rapid and selective labeling of native proteins. This novel electrophile can be customized with a wide range of different protein ligands and is particularly well‐suited for the labeling and imaging of transmembrane proteins. With this probe design, the identity and relative levels of basal and hypoxia‐induced transmembrane carbonic anhydrases were revealed by live cell imaging and in‐gel fluorescence analysis. We believe that the extension of this difluorophenyl ester reactive module would allow for the specific labeling of various endogenous membrane proteins, facilitating in‐depth studies of their distribution and functions in biological processes.
Endogenous protein labeling: Affinity‐based protein labeling probes based on an ortho‐difluorophenyl ester reactive module exhibit long term stability in stock solution and aqueous buffer, yet they can undergo rapid and selective modification of native proteins in living cells. |
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AbstractList | Abstract
The long‐term stability of affinity‐based protein labeling probes is crucial to obtain reproducible protein labeling results. However, highly stable probes generally suffer from low protein labeling efficiency and pose significant challenges when labeling low abundance native proteins in living cells. In this paper, we report that protein labeling probes based on an ortho‐difluorophenyl ester reactive module exhibit long‐term stability in DMSO stock solution and aqueous buffer, yet they can undergo rapid and selective labeling of native proteins. This novel electrophile can be customized with a wide range of different protein ligands and is particularly well‐suited for the labeling and imaging of transmembrane proteins. With this probe design, the identity and relative levels of basal and hypoxia‐induced transmembrane carbonic anhydrases were revealed by live cell imaging and in‐gel fluorescence analysis. We believe that the extension of this difluorophenyl ester reactive module would allow for the specific labeling of various endogenous membrane proteins, facilitating in‐depth studies of their distribution and functions in biological processes. The long-term stability of affinity-based protein labeling probes is crucial to obtain reproducible protein labeling results. However, highly stable probes generally suffer from low protein labeling efficiency and pose significant challenges when labeling low abundance native proteins in living cells. In this paper, we report that protein labeling probes based on an ortho-difluorophenyl ester reactive module exhibit long-term stability in DMSO stock solution and aqueous buffer, yet they can undergo rapid and selective labeling of native proteins. This novel electrophile can be customized with a wide range of different protein ligands and is particularly well-suited for the labeling and imaging of transmembrane proteins. With this probe design, the identity and relative levels of basal and hypoxia-induced transmembrane carbonic anhydrases were revealed by live cell imaging and in-gel fluorescence analysis. We believe that the extension of this difluorophenyl ester reactive module would allow for the specific labeling of various endogenous membrane proteins, facilitating in-depth studies of their distribution and functions in biological processes. The long‐term stability of affinity‐based protein labeling probes is crucial to obtain reproducible protein labeling results. However, highly stable probes generally suffer from low protein labeling efficiency and pose significant challenges when labeling low abundance native proteins in living cells. In this paper, we report that protein labeling probes based on an ortho‐difluorophenyl ester reactive module exhibit long‐term stability in DMSO stock solution and aqueous buffer, yet they can undergo rapid and selective labeling of native proteins. This novel electrophile can be customized with a wide range of different protein ligands and is particularly well‐suited for the labeling and imaging of transmembrane proteins. With this probe design, the identity and relative levels of basal and hypoxia‐induced transmembrane carbonic anhydrases were revealed by live cell imaging and in‐gel fluorescence analysis. We believe that the extension of this difluorophenyl ester reactive module would allow for the specific labeling of various endogenous membrane proteins, facilitating in‐depth studies of their distribution and functions in biological processes. Endogenous protein labeling: Affinity‐based protein labeling probes based on an ortho‐difluorophenyl ester reactive module exhibit long term stability in stock solution and aqueous buffer, yet they can undergo rapid and selective modification of native proteins in living cells. |
Author | Ru Hwu, Jih Chan, Hsin‐Ju Lin, Xin‐Hui Fan, Syuan‐Yun Tan, Kui‐Thong |
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Snippet | The long‐term stability of affinity‐based protein labeling probes is crucial to obtain reproducible protein labeling results. However, highly stable probes... The long-term stability of affinity-based protein labeling probes is crucial to obtain reproducible protein labeling results. However, highly stable probes... Abstract The long‐term stability of affinity‐based protein labeling probes is crucial to obtain reproducible protein labeling results. However, highly stable... |
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SubjectTerms | Affinity Biological activity Cell Line, Tumor Cells (biology) Chemistry Difluorophenyl ester Esters - chemical synthesis Esters - chemistry Fluorescence Fluorescent Dyes - chemical synthesis Fluorescent Dyes - chemistry Fluorescent Imaging Humans Hydrocarbons, Fluorinated - chemical synthesis Hydrocarbons, Fluorinated - chemistry Hypoxia Labeling Membrane proteins Membrane Proteins - analysis Modules Molecular Structure Optical Imaging Protein labeling Proteins Stability Staining and Labeling |
Title | Rapid and Selective Labeling of Endogenous Transmembrane Proteins in Living Cells with a Difluorophenyl Ester Affinity‐Based Probe |
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