A Magnetically Actuated Microscaffold Containing Mesenchymal Stem Cells for Articular Cartilage Repair
This study proposes a magnetically actuated microscaffold with the capability of targeted mesenchymal stem cell (MSC) delivery for articular cartilage regeneration. The microscaffold, as a 3D porous microbead, is divided into body and surface portions according to its materials and fabrication metho...
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Published in | Advanced healthcare materials Vol. 6; no. 13 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Germany
01.07.2017
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Abstract | This study proposes a magnetically actuated microscaffold with the capability of targeted mesenchymal stem cell (MSC) delivery for articular cartilage regeneration. The microscaffold, as a 3D porous microbead, is divided into body and surface portions according to its materials and fabrication methods. The microscaffold body, which consists of poly(lactic-co-glycolic acid) (PLGA), is formed through water-in-oil-in-water emulsion templating, and its surface is coated with amine functionalized magnetic nanoparticles (MNPs) via amino bond formation. The porous PLGA structure of the microscaffold can assist in cell adhesion and migration, and the MNPs on the microscaffold can make it possible to steer using an electromagnetic actuation system that provides external magnetic fields for the 3D locomotion of the microscaffold. As a fundamental test of the magnetic response of the microscaffold, it is characterized in terms of the magnetization curve, velocity, and 3D locomotion of a single microscaffold. In addition, its function with a cargo of MSCs for cartilage regeneration is demonstrated from the proliferation, viability, and chondrogenic differentiation of D1 mouse MSCs that are cultured on the microscaffold. For the feasibility tests for cartilage repair, 2D/3D targeting of multiple microscaffolds with the MSCs is performed to demonstrate targeted stem cell delivery using the microscaffolds and their swarm motion. |
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AbstractList | This study proposes a magnetically actuated microscaffold with the capability of targeted mesenchymal stem cell (MSC) delivery for articular cartilage regeneration. The microscaffold, as a 3D porous microbead, is divided into body and surface portions according to its materials and fabrication methods. The microscaffold body, which consists of poly(lactic-co-glycolic acid) (PLGA), is formed through water-in-oil-in-water emulsion templating, and its surface is coated with amine functionalized magnetic nanoparticles (MNPs) via amino bond formation. The porous PLGA structure of the microscaffold can assist in cell adhesion and migration, and the MNPs on the microscaffold can make it possible to steer using an electromagnetic actuation system that provides external magnetic fields for the 3D locomotion of the microscaffold. As a fundamental test of the magnetic response of the microscaffold, it is characterized in terms of the magnetization curve, velocity, and 3D locomotion of a single microscaffold. In addition, its function with a cargo of MSCs for cartilage regeneration is demonstrated from the proliferation, viability, and chondrogenic differentiation of D1 mouse MSCs that are cultured on the microscaffold. For the feasibility tests for cartilage repair, 2D/3D targeting of multiple microscaffolds with the MSCs is performed to demonstrate targeted stem cell delivery using the microscaffolds and their swarm motion. |
Author | Yoo, Ami Jeon, Mi-Jeong Park, Jong-Oh Han, Jiwon Park, Sukho Zhen, Jin Go, Gwangjun Zheng, Shaohui |
Author_xml | – sequence: 1 givenname: Gwangjun surname: Go fullname: Go, Gwangjun organization: School of Mechanical Systems Engineering, Chonnam National University, Gwangju, 500-757, South Korea – sequence: 2 givenname: Jiwon surname: Han fullname: Han, Jiwon organization: Medical Microrobot Center (MRC), Robot Research Initiative (RRI), Chonnam National University, Gwangju, 500-480, South Korea – sequence: 3 givenname: Jin surname: Zhen fullname: Zhen, Jin organization: School of Mechanical Systems Engineering, Chonnam National University, Gwangju, 500-757, South Korea – sequence: 4 givenname: Shaohui surname: Zheng fullname: Zheng, Shaohui organization: School of Mechanical Systems Engineering, Chonnam National University, Gwangju, 500-757, South Korea – sequence: 5 givenname: Ami surname: Yoo fullname: Yoo, Ami organization: Medical Microrobot Center (MRC), Robot Research Initiative (RRI), Chonnam National University, Gwangju, 500-480, South Korea – sequence: 6 givenname: Mi-Jeong surname: Jeon fullname: Jeon, Mi-Jeong organization: Medical Microrobot Center (MRC), Robot Research Initiative (RRI), Chonnam National University, Gwangju, 500-480, South Korea – sequence: 7 givenname: Jong-Oh surname: Park fullname: Park, Jong-Oh organization: School of Mechanical Systems Engineering, Chonnam National University, Gwangju, 500-757, South Korea – sequence: 8 givenname: Sukho surname: Park fullname: Park, Sukho organization: Department of Robotics Engineering, Daegu Gyeongbuk Institute of Science and Technology, Daegu, 711-873, South Korea |
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Keywords | articular cartilage repair magnetic field mesenchymal stem cells porous scaffold beads |
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SubjectTerms | Cartilage, Articular - cytology Cartilage, Articular - metabolism Humans Lactic Acid - chemistry Magnetic Fields Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism Polyglycolic Acid - chemistry Tissue Scaffolds - chemistry |
Title | A Magnetically Actuated Microscaffold Containing Mesenchymal Stem Cells for Articular Cartilage Repair |
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