Profiling the progression of cancer: Separation of microsomal proteins in MCF10 breast epithelial cell lines using nonporous chromatophoresis

• Published in: Proteomics (Weinheim) Vol. 3; no. 7; pp. 1256 - 1269
• Main Authors: O'Neil, Kimberly A., Miller, Fred R., Barder, Timothy J., Lubman, David M.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.07.2003; WILEY‐VCH Verlag
• Subjects: Breast - metabolism; Breast cancer; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cell Line; Cell Line, Tumor; Cell Membrane - metabolism; Chromatography, High Pressure Liquid; Disease Progression; Electrophoresis, Polyacrylamide Gel; Epithelial Cells - metabolism; Humans; Liquid chromatography; Membrane protein; Microsomes - metabolism; Protein Array Analysis - methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Time Factors; Trypsin - pharmacology

The heterogeneity of cellular protein expression has stimulated development of separations targeting smaller groups of related proteins rather than entire proteomes. The following work describes the development of a technique for the characterization of membrane subproteomes from five different brea...