Fluorescent Triazole Urea Activity‐Based Probes for the Single‐Cell Phenotypic Characterization of Staphylococcus aureus

• Published in: Angewandte Chemie International Edition Vol. 58; no. 17; pp. 5643 - 5647
• Main Authors: Chen, Linhai, Keller, Laura J., Cordasco, Edward, Bogyo, Matthew, Lentz, Christian S.
• Format: Journal Article
• Language: English
• Published: WEINHEIM Wiley 16.04.2019; Wiley Subscription Services, Inc
• Edition: International ed. in English
• Subjects: activity-based probes; Antibiotic resistance; Antibiotics; Antifungal agents; Chemical activity; Chemical synthesis; Chemistry; Chemistry, Multidisciplinary; Enzymatic activity; Enzyme activity; Enzymes; Fluorescence; Fluorescent Dyes - metabolism; Fluorescent indicators; Heterogeneity; Humans; molecular imaging; Organic chemistry; Phenotype; Phenotyping; Physical Sciences; Probes; protein profiling; Proteins; Science & Technology; Serine; single-cell analysis; Staphylococcus aureus; Staphylococcus aureus - metabolism; Triazoles; Triazoles - metabolism; Urea; Urea - metabolism; Virulence; enzymes; activity-based probes; molecular imaging; protein profiling; single-cell analysis

Phenotypically distinct cellular (sub)populations are clinically relevant for the virulence and antibiotic resistance of a bacterial pathogen, but functionally different cells are usually indistinguishable from each other. Herein, we introduce fluorescent activity‐based probes as chemical tools for...