An introduction to DNA microarrays for gene expression analysis

This tutorial presents a basic introduction to DNA microarrays as employed for gene expression analysis, approaching the subject from a chemometrics perspective. The emphasis is on describing the nature of the measurement process, from the platforms used to a few of the standard higher-level data an...

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Published inChemometrics and intelligent laboratory systems Vol. 104; no. 1; pp. 28 - 52
Main Authors Karakach, Tobias K., Flight, Robert M., Douglas, Susan E., Wentzell, Peter D.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 15.11.2010
Subjects
DNA microarray
Experimental design
Gene expression
GeneChip
DNA microarray
GeneChip
Gene expression
Experimental design
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Summary:This tutorial presents a basic introduction to DNA microarrays as employed for gene expression analysis, approaching the subject from a chemometrics perspective. The emphasis is on describing the nature of the measurement process, from the platforms used to a few of the standard higher-level data analysis tools employed. Topics include experimental design, detection, image processing, measurement errors, ratio calculation, background correction, normalization, and higher-level data processing. The objective is to present the chemometrician with as clear a picture as possible of an evolving technology so that the strengths and limitations of DNA microarrays are appreciated. Although the focus is primarily on spotted, two-color microarrays, a significant discussion of single-channel, lithographic arrays is also included.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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ISSN:0169-7439
1873-3239
DOI:10.1016/j.chemolab.2010.04.003