An introduction to DNA microarrays for gene expression analysis
This tutorial presents a basic introduction to DNA microarrays as employed for gene expression analysis, approaching the subject from a chemometrics perspective. The emphasis is on describing the nature of the measurement process, from the platforms used to a few of the standard higher-level data an...
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| Published in | Chemometrics and intelligent laboratory systems Vol. 104; no. 1; pp. 28 - 52 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
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Elsevier B.V
15.11.2010
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| Subjects | |
| Online Access | Get full text |
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| Abstract | This tutorial presents a basic introduction to DNA microarrays as employed for gene expression analysis, approaching the subject from a chemometrics perspective. The emphasis is on describing the nature of the measurement process, from the platforms used to a few of the standard higher-level data analysis tools employed. Topics include experimental design, detection, image processing, measurement errors, ratio calculation, background correction, normalization, and higher-level data processing. The objective is to present the chemometrician with as clear a picture as possible of an evolving technology so that the strengths and limitations of DNA microarrays are appreciated. Although the focus is primarily on spotted, two-color microarrays, a significant discussion of single-channel, lithographic arrays is also included. |
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| AbstractList | This tutorial presents a basic introduction to DNA microarrays as employed for gene expression analysis, approaching the subject from a chemometrics perspective. The emphasis is on describing the nature of the measurement process, from the platforms used to a few of the standard higher-level data analysis tools employed. Topics include experimental design, detection, image processing, measurement errors, ratio calculation, background correction, normalization, and higher-level data processing. The objective is to present the chemometrician with as clear a picture as possible of an evolving technology so that the strengths and limitations of DNA microarrays are appreciated. Although the focus is primarily on spotted, two-color microarrays, a significant discussion of single-channel, lithographic arrays is also included. |
| Author | Flight, Robert M. Wentzell, Peter D. Douglas, Susan E. Karakach, Tobias K. |
| Author_xml | – sequence: 1 givenname: Tobias K. surname: Karakach fullname: Karakach, Tobias K. organization: Institute of Marine Biosciences, National Research Council of Canada, 1411 Oxford Street, Halifax, Nova Scotia, Canada B3H 3Z1 – sequence: 2 givenname: Robert M. surname: Flight fullname: Flight, Robert M. organization: Department of Chemistry, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4J3 – sequence: 3 givenname: Susan E. surname: Douglas fullname: Douglas, Susan E. organization: Institute of Marine Biosciences, National Research Council of Canada, 1411 Oxford Street, Halifax, Nova Scotia, Canada B3H 3Z1 – sequence: 4 givenname: Peter D. surname: Wentzell fullname: Wentzell, Peter D. email: peter.wentzell@dal.ca organization: Department of Chemistry, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4J3 |
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