Localization of type I iodothyronine 5'-deiodinase to the basolateral plasma membrane in renal cortical epithelial cells

• Published in: The Journal of biological chemistry Vol. 266; no. 17; pp. 11262 - 11269
• Main Authors: LEONARD, J. L, EKENBARGER, D. M, FRANK, S. J, FARWELL, A. P, KOEHRLE, J
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Biochemistry and Molecular Biology 15.06.1991
• Subjects: Affinity Labels - metabolism; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Cell Line; Cell Membrane - enzymology; Detergents; Enzymes and enzyme inhibitors; Epithelium - enzymology; Fundamental and applied biological sciences. Psychology; Iodide Peroxidase - isolation & purification; Iodide Peroxidase - metabolism; Isoenzymes - isolation & purification; Isoenzymes - metabolism; Kidney Cortex - enzymology; Kidney Glomerulus - enzymology; Kinetics; Miscellaneous; Molecular Weight; Rats; Subcellular Fractions - enzymology; Thyroxine - analogs & derivatives; Thyroxine - metabolism; Vertebrata; Mammalia; Enzymatic activity; Radiolabelling; Rat; Rodentia; Epithelial cell; Laterobasal membrane; Localization

Type I iodothyronine 5'-deiodinase is an integral membrane protein catalyzing the phenolic ring deiodination of thyroxine. We recently showed that the substrate binding subunit of this approximately 50-kDa protein is selectively labeled with N-bromoacetyl-L-thyroxine, allowing ready identificat...