Divergence of Mechanisms Regulating Respiratory Burst in Blood and Sputum Eosinophils and Neutrophils from Atopic Subjects

• Published in: The Journal of immunology (1950) Vol. 170; no. 5; pp. 2670 - 2679
• Main Authors: Lacy, Paige, Latif, Dalia Abdel, Steward, Melissa, Musat-Marcu, Sorin, Man, S. F. Paul, Moqbel, Redwan
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 01.03.2003
• Subjects: Asthma - metabolism; Asthma - pathology; cdc42 GTP-Binding Protein - biosynthesis; cdc42 GTP-Binding Protein - blood; cdc42 GTP-Binding Protein - genetics; Cell Membrane - enzymology; Enzyme Activation - physiology; Eosinophils - enzymology; Eosinophils - metabolism; Eosinophils - pathology; Extracellular Space - metabolism; Humans; Hypersensitivity, Immediate - blood; Hypersensitivity, Immediate - enzymology; Hypersensitivity, Immediate - metabolism; Hypersensitivity, Immediate - pathology; Immunohistochemistry; Intracellular Fluid - metabolism; Membrane Transport Proteins; NADPH Dehydrogenase - blood; NADPH Dehydrogenase - metabolism; NADPH Oxidases - blood; NADPH Oxidases - metabolism; Neutrophils - metabolism; Neutrophils - pathology; Phosphoproteins - blood; Phosphoproteins - metabolism; Protein Processing, Post-Translational; Protein Transport; rac GTP-Binding Proteins - biosynthesis; rac GTP-Binding Proteins - blood; rac GTP-Binding Proteins - genetics; rac GTP-Binding Proteins - metabolism; rac1 GTP-Binding Protein - biosynthesis; rac1 GTP-Binding Protein - blood; rac1 GTP-Binding Protein - genetics; RAC2 GTP-Binding Protein; Respiratory Burst - physiology; rho GTP-Binding Proteins - blood; rho GTP-Binding Proteins - metabolism; RNA, Messenger - biosynthesis; Sputum - cytology; Sputum - enzymology; Sputum - metabolism; Superoxides - blood; Superoxides - metabolism

Eosinophil respiratory burst is an important event in asthma and related inflammatory disorders. However, little is known concerning activation of the respiratory burst NADPH oxidase in human eosinophils. Conversely, neutrophils are known to assemble NADPH oxidase in intracellular and plasma membran...