Oligosaccharide elicitor prepared from Salecan triggers the defense responses of Arabidopsis thaliana Col0 against Botrytis cinerea infection

• Published in: World journal of microbiology & biotechnology Vol. 33; no. 9; p. 165
• Main Authors: Xu, Haiyang, Li, Jing, Wang, Shiming, Xu, Linxiang, Cheng, Rui, Zhang, Jianfa
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.09.2017; Springer Nature B.V
• Subjects: Activation analysis; Applied Microbiology; Arabidopsis - drug effects; Arabidopsis - growth & development; Arabidopsis - microbiology; Arabidopsis Proteins; beta-Glucans - chemistry; Biochemistry; Biological control; Biomedical and Life Sciences; Biotechnology; Botrytis - pathogenicity; Disease Resistance; Environmental Engineering/Biotechnology; Gel filtration; Gene Expression Regulation, Plant - drug effects; Glucan; Hydrogen peroxide; Hydrolysis; Jasmonic acid; Leaves; Lesions; Life Sciences; Microbiology; Oligosaccharides; Oligosaccharides - chemistry; Oligosaccharides - pharmacology; Original Paper; Pathogenesis; Pathogenesis-related proteins; Plant Diseases - microbiology; Plant Diseases - prevention & control; Plant growth; Plant Leaves - drug effects; Plant resistance; Plants (botany); Polymerase chain reaction; Pretreatment; Proteins; Salicylic acid; Seedlings; Seedlings - drug effects; Signal transduction; Transcription; β-Glucan; Salicylic acid; Plant protection; Salecan oligosaccharide; Jasmonate; Necrotroph
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-017-2333-3

Oligosaccharides from the water-soluble β-glucan, Salecan, were investigated to evaluate the activation effect on the defense responses of Arabidopsis thaliana Col0. Salecan oligosaccharides (ScOs, DP 5–10) were prepared at first by acid hydrolysis and gel filtration chromatography and then employed for foliar spray on Arabidopsis seedlings and plants. After ScOs treatment, increase of hydrogen peroxide was histologically and analytically detected in leaves. Transcription levels of several indicator genes which correspond to the signaling pathways and pathogenesis-related proteins were up-regulated at different time by means of quantitative RT-PCR analysis. Importantly, the sequential activation of salicylic acid-mediated and jasmonate-mediated signaling pathway was observed in leaves. Furthermore, pot test and antimicrobial test gave the fact that pretreatment with ScOs restrained the leaf lesions caused by infection of Botrytis cinerea Bc0510 via the enhancement of defense responses of A. thaliana . In conclusion, Salecan oligosaccharides serve as an elicitor which can be used for biological control of plant pathogen. Graphical abstract