References Values of Soluble α-Klotho Serum Levels Using an Enzyme-Linked Immunosorbent Assay in Healthy Adults Aged 18–85 Years
α-Klotho protein is a powerful predictor of the aging process and lifespan. Although lowered circulating soluble α-Klotho levels have been observed in aged non-healthy individuals, no specific reference values across a wide range of ages and sex using an enzyme-linked immunosorbent assay (ELISA) are...
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| Published in | Journal of clinical medicine Vol. 11; no. 9; p. 2415 |
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| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
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25.04.2022
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| Abstract | α-Klotho protein is a powerful predictor of the aging process and lifespan. Although lowered circulating soluble α-Klotho levels have been observed in aged non-healthy individuals, no specific reference values across a wide range of ages and sex using an enzyme-linked immunosorbent assay (ELISA) are available for larger cohorts of healthy individuals. The present analytical cross-sectional study was aimed to establish the reference values of soluble α-Klotho serum levels in healthy adults by age and sex groups. A total of 346 (59% women) healthy individuals aged from 18 to 85 years were recruited. Subjects were divided by sex and age as: (i) young (18–34.9 years), (ii) middle-aged (35–54.9 years), and (iii) senior (55–85 years) individuals. The soluble α-Klotho levels were measured in serum using ELISA. Senior adults were the age-group that presented the lowest soluble α-Klotho serum levels (p < 0.01), with age showing a negative association with soluble α-Klotho serum levels (p < 0.001). No differences between sexes were observed. Therefore, soluble α-Klotho levels were especially decreased—regardless of sex—in our cohort of healthy individuals because of the physiological decline derived from the aging process. We recommend routine assessments of soluble α-Klotho levels using ELISA as a simple and cheap detectable marker of aging that improves quality of life in the elderly. |
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| AbstractList | α-Klotho protein is a powerful predictor of the aging process and lifespan. Although lowered circulating soluble α-Klotho levels have been observed in aged non-healthy individuals, no specific reference values across a wide range of ages and sex using an enzyme-linked immunosorbent assay (ELISA) are available for larger cohorts of healthy individuals. The present analytical cross-sectional study was aimed to establish the reference values of soluble α-Klotho serum levels in healthy adults by age and sex groups. A total of 346 (59% women) healthy individuals aged from 18 to 85 years were recruited. Subjects were divided by sex and age as: (i) young (18–34.9 years), (ii) middle-aged (35–54.9 years), and (iii) senior (55–85 years) individuals. The soluble α-Klotho levels were measured in serum using ELISA. Senior adults were the age-group that presented the lowest soluble α-Klotho serum levels (p < 0.01), with age showing a negative association with soluble α-Klotho serum levels (p < 0.001). No differences between sexes were observed. Therefore, soluble α-Klotho levels were especially decreased—regardless of sex—in our cohort of healthy individuals because of the physiological decline derived from the aging process. We recommend routine assessments of soluble α-Klotho levels using ELISA as a simple and cheap detectable marker of aging that improves quality of life in the elderly. α-Klotho protein is a powerful predictor of the aging process and lifespan. Although lowered circulating soluble α-Klotho levels have been observed in aged non-healthy individuals, no specific reference values across a wide range of ages and sex using an enzyme-linked immunosorbent assay (ELISA) are available for larger cohorts of healthy individuals. The present analytical cross-sectional study was aimed to establish the reference values of soluble α-Klotho serum levels in healthy adults by age and sex groups. A total of 346 (59% women) healthy individuals aged from 18 to 85 years were recruited. Subjects were divided by sex and age as: (i) young (18−34.9 years), (ii) middle-aged (35−54.9 years), and (iii) senior (55−85 years) individuals. The soluble α-Klotho levels were measured in serum using ELISA. Senior adults were the age-group that presented the lowest soluble α-Klotho serum levels (p < 0.01), with age showing a negative association with soluble α-Klotho serum levels (p < 0.001). No differences between sexes were observed. Therefore, soluble α-Klotho levels were especially decreased—regardless of sex—in our cohort of healthy individuals because of the physiological decline derived from the aging process. We recommend routine assessments of soluble α-Klotho levels using ELISA as a simple and cheap detectable marker of aging that improves quality of life in the elderly.α-Klotho protein is a powerful predictor of the aging process and lifespan. Although lowered circulating soluble α-Klotho levels have been observed in aged non-healthy individuals, no specific reference values across a wide range of ages and sex using an enzyme-linked immunosorbent assay (ELISA) are available for larger cohorts of healthy individuals. The present analytical cross-sectional study was aimed to establish the reference values of soluble α-Klotho serum levels in healthy adults by age and sex groups. A total of 346 (59% women) healthy individuals aged from 18 to 85 years were recruited. Subjects were divided by sex and age as: (i) young (18−34.9 years), (ii) middle-aged (35−54.9 years), and (iii) senior (55−85 years) individuals. The soluble α-Klotho levels were measured in serum using ELISA. Senior adults were the age-group that presented the lowest soluble α-Klotho serum levels (p < 0.01), with age showing a negative association with soluble α-Klotho serum levels (p < 0.001). No differences between sexes were observed. Therefore, soluble α-Klotho levels were especially decreased—regardless of sex—in our cohort of healthy individuals because of the physiological decline derived from the aging process. We recommend routine assessments of soluble α-Klotho levels using ELISA as a simple and cheap detectable marker of aging that improves quality of life in the elderly. α-Klotho protein is a powerful predictor of the aging process and lifespan. Although lowered circulating soluble α-Klotho levels have been observed in aged non-healthy individuals, no specific reference values across a wide range of ages and sex using an enzyme-linked immunosorbent assay (ELISA) are available for larger cohorts of healthy individuals. The present analytical cross-sectional study was aimed to establish the reference values of soluble α-Klotho serum levels in healthy adults by age and sex groups. A total of 346 (59% women) healthy individuals aged from 18 to 85 years were recruited. Subjects were divided by sex and age as: (i) young (18–34.9 years), (ii) middle-aged (35–54.9 years), and (iii) senior (55–85 years) individuals. The soluble α-Klotho levels were measured in serum using ELISA. Senior adults were the age-group that presented the lowest soluble α-Klotho serum levels ( p < 0.01), with age showing a negative association with soluble α-Klotho serum levels ( p < 0.001). No differences between sexes were observed. Therefore, soluble α-Klotho levels were especially decreased—regardless of sex—in our cohort of healthy individuals because of the physiological decline derived from the aging process. We recommend routine assessments of soluble α-Klotho levels using ELISA as a simple and cheap detectable marker of aging that improves quality of life in the elderly. α-Klotho protein is a powerful predictor of the aging process and lifespan. Although lowered circulating soluble α-Klotho levels have been observed in aged non-healthy individuals, no specific reference values across a wide range of ages and sex using an enzyme-linked immunosorbent assay (ELISA) are available for larger cohorts of healthy individuals. The present analytical cross-sectional study was aimed to establish the reference values of soluble α-Klotho serum levels in healthy adults by age and sex groups. A total of 346 (59% women) healthy individuals aged from 18 to 85 years were recruited. Subjects were divided by sex and age as: (i) young (18−34.9 years), (ii) middle-aged (35−54.9 years), and (iii) senior (55−85 years) individuals. The soluble α-Klotho levels were measured in serum using ELISA. Senior adults were the age-group that presented the lowest soluble α-Klotho serum levels (p < 0.01), with age showing a negative association with soluble α-Klotho serum levels (p < 0.001). No differences between sexes were observed. Therefore, soluble α-Klotho levels were especially decreased—regardless of sex—in our cohort of healthy individuals because of the physiological decline derived from the aging process. We recommend routine assessments of soluble α-Klotho levels using ELISA as a simple and cheap detectable marker of aging that improves quality of life in the elderly. |
| Author | de Haro-Muñoz, Tomás Díaz-Alberola, Irene Amaro-Gahete, Francisco J. Vázquez-Lorente, Héctor Jurado-Fasoli, Lucas Castillo, Manuel J. López-Velez, María del Señor de Haro-Romero, Teresa Espuch-Oliver, Andrea |
| AuthorAffiliation | 3 PROmoting FITness and Health through Physical Activity Research Group (PROFITH), Department of Physical Education and Sports, Faculty of Sport Sciences, University of Granada, 18007 Granada, Spain 1 Unidad de Gestión Clínica de Laboratorios Clínicos, Hospital H.U. San Cecilio, Ibs. Granada, Complejo Hospitalario de Granada, 18016 Granada, Spain; andreaespuch@gmail.com (A.E.-O.); tomas.haro.sspa@juntadeandalucia.es (T.d.H.-M.); msenor.lopez.sspa@juntadeandalucia.es (M.d.S.L.-V.); mariat.haro.sspa@juntadeandalucia.es (T.d.H.-R.) 2 Department of Physiology, University of Granada, 18071 Granada, Spain; juradofasoli@ugr.es (L.J.-F.); mcgarzon@ugr.es (M.J.C.) 4 Servicio de Análisis Clínicos e Inmunología, Unidad de Gestión Clínica, Laboratorio Clínico, Hospital Universitario Virgen de las Nieves, 18014 Granada, Spain; idiazalberola@gmail.com 5 Instituto de Investigación Biosanitaria de Granada (ibs.Granada), 18016 Granada, Spain |
| AuthorAffiliation_xml | – name: 5 Instituto de Investigación Biosanitaria de Granada (ibs.Granada), 18016 Granada, Spain – name: 3 PROmoting FITness and Health through Physical Activity Research Group (PROFITH), Department of Physical Education and Sports, Faculty of Sport Sciences, University of Granada, 18007 Granada, Spain – name: 4 Servicio de Análisis Clínicos e Inmunología, Unidad de Gestión Clínica, Laboratorio Clínico, Hospital Universitario Virgen de las Nieves, 18014 Granada, Spain; idiazalberola@gmail.com – name: 2 Department of Physiology, University of Granada, 18071 Granada, Spain; juradofasoli@ugr.es (L.J.-F.); mcgarzon@ugr.es (M.J.C.) – name: 1 Unidad de Gestión Clínica de Laboratorios Clínicos, Hospital H.U. San Cecilio, Ibs. Granada, Complejo Hospitalario de Granada, 18016 Granada, Spain; andreaespuch@gmail.com (A.E.-O.); tomas.haro.sspa@juntadeandalucia.es (T.d.H.-M.); msenor.lopez.sspa@juntadeandalucia.es (M.d.S.L.-V.); mariat.haro.sspa@juntadeandalucia.es (T.d.H.-R.) |
| Author_xml | – sequence: 1 givenname: Andrea orcidid: 0000-0001-9013-3621 surname: Espuch-Oliver fullname: Espuch-Oliver, Andrea – sequence: 2 givenname: Héctor orcidid: 0000-0003-0183-6902 surname: Vázquez-Lorente fullname: Vázquez-Lorente, Héctor – sequence: 3 givenname: Lucas orcidid: 0000-0002-5254-1816 surname: Jurado-Fasoli fullname: Jurado-Fasoli, Lucas – sequence: 4 givenname: Tomás surname: de Haro-Muñoz fullname: de Haro-Muñoz, Tomás – sequence: 5 givenname: Irene orcidid: 0000-0002-8458-612X surname: Díaz-Alberola fullname: Díaz-Alberola, Irene – sequence: 6 givenname: María del Señor surname: López-Velez fullname: López-Velez, María del Señor – sequence: 7 givenname: Teresa orcidid: 0000-0001-8647-2831 surname: de Haro-Romero fullname: de Haro-Romero, Teresa – sequence: 8 givenname: Manuel J. orcidid: 0000-0002-1196-9488 surname: Castillo fullname: Castillo, Manuel J. – sequence: 9 givenname: Francisco J. orcidid: 0000-0002-7207-9016 surname: Amaro-Gahete fullname: Amaro-Gahete, Francisco J. |
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| Cites_doi | 10.3390/ijms222212432 10.1210/clinem/dgz232 10.18632/aging.102771 10.1186/s12983-018-0282-9 10.1186/s42826-022-00113-8 10.1111/cge.13837 10.1515/cclm-2012-0682 10.1016/j.clinbiochem.2013.05.046 10.1038/labinvest.2015.86 10.3389/fphys.2014.00189 10.1042/NS20200101 10.18632/aging.102100 10.1515/jpem-2017-0357 10.3892/mco.2021.2343 10.3233/BPL-170057 10.1097/MNH.0b013e32835422ad 10.1210/clinem/dgab257 10.3390/ijms22010043 10.1309/AJCPGPMAF7SFRBO4 10.1177/1479164120970901 10.3389/fphar.2017.00950 10.3390/biom12010070 10.1016/j.bbrc.2010.06.110 10.18632/aging.102734 10.1038/s41598-020-69296-1 10.3390/nu12020281 10.1681/ASN.2015121308 10.3389/fmed.2021.672000 10.1371/journal.pone.0220796 10.2147/CIA.S84978 10.1016/j.exger.2021.111518 10.1371/journal.pone.0107174 10.1016/j.celrep.2017.07.024 10.1016/j.bbagen.2009.02.005 10.1093/ckj/sfz085 |
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| Keywords | aging references values α-Klotho protein ELISA |
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| References | Leon (ref_7) 2017; 20 Veronesi (ref_32) 2021; 154 Yamazaki (ref_17) 2010; 398 Yahya (ref_36) 2021; 15 Dhayat (ref_12) 2019; 105 Rueda (ref_1) 2020; 177 Hughes (ref_25) 2017; 28 Ruiz (ref_8) 2019; 11 Saghiv (ref_31) 2017; 6 Xiao (ref_22) 2004; 117 ref_13 Castillo (ref_9) 2020; 12 Ferri (ref_11) 2020; 12 ref_33 ref_10 (ref_4) 2009; 1790 Pedersen (ref_18) 2013; 46 Corcillo (ref_34) 2020; 17 Hanson (ref_29) 2021; 5 Avin (ref_30) 2014; 5 Schnabel (ref_20) 2021; 99 ref_37 Neyra (ref_23) 2019; 13 Devaraj (ref_19) 2012; 137 Yi (ref_28) 2022; 38 Vo (ref_26) 2018; 3 Schweizer (ref_16) 2021; 106 Hu (ref_24) 2015; 10 Wojcicki (ref_15) 2018; 31 ref_3 Zhou (ref_38) 2018; 8 Ji (ref_27) 2018; 16 Behringer (ref_14) 2018; 15 Tang (ref_35) 2015; 96 Cai (ref_2) 2021; 8 Batlahally (ref_5) 2020; 10 (ref_39) 2012; 21 Siest (ref_21) 2012; 51 ref_6 |
| References_xml | – ident: ref_13 doi: 10.3390/ijms222212432 – volume: 105 start-page: e1135 year: 2019 ident: ref_12 article-title: Parathyroid Hormone and Plasma Phosphate Are Predictors of Soluble α-Klotho Levels in Adults of European Descent publication-title: J. Clin. Endocrinol. Metab. doi: 10.1210/clinem/dgz232 – volume: 12 start-page: 2698 year: 2020 ident: ref_9 article-title: Relationship between plasma S-Klotho and cardiometabolic risk in sedentary adults publication-title: Aging doi: 10.18632/aging.102771 – volume: 15 start-page: 35 year: 2018 ident: ref_14 article-title: Aging and sex affect soluble alpha klotho levels in bonobos and chimpanzees publication-title: Front. Zool. doi: 10.1186/s12983-018-0282-9 – volume: 38 start-page: 3 year: 2022 ident: ref_28 article-title: Disease predictability review using common biomarkers appearing in diabetic nephropathy and neurodegeneration of experimental animals publication-title: Lab. Anim. Res. doi: 10.1186/s42826-022-00113-8 – volume: 99 start-page: 3 year: 2021 ident: ref_20 article-title: Premature aging disorders: A clinical and genetic compendium publication-title: Clin. Genet. doi: 10.1111/cge.13837 – volume: 51 start-page: 47 year: 2012 ident: ref_21 article-title: The theory of reference values: An unfinished symphony publication-title: Clin. Chem. Lab. Med. (CCLM) doi: 10.1515/cclm-2012-0682 – volume: 46 start-page: 1079 year: 2013 ident: ref_18 article-title: Soluble serum Klotho levels in healthy subjects. Comparison of two different immunoassays publication-title: Clin. Biochem. doi: 10.1016/j.clinbiochem.2013.05.046 – volume: 96 start-page: 197 year: 2015 ident: ref_35 article-title: Klotho: A tumor suppressor and modulator of the Wnt/β-catenin pathway in human hepatocellular carcinoma publication-title: Lab. Investig. doi: 10.1038/labinvest.2015.86 – volume: 117 start-page: 742 year: 2004 ident: ref_22 article-title: Klotho is a serum factor related to human aging publication-title: Chin. Med. J. – volume: 5 start-page: 189 year: 2014 ident: ref_30 article-title: Skeletal muscle as a regulator of the longevity protein, Klotho publication-title: Front. Physiol. doi: 10.3389/fphys.2014.00189 – volume: 5 start-page: 20200101 year: 2021 ident: ref_29 article-title: Exploiting the neuroprotective effects of α-klotho to tackle ageing- and neurodegeneration-related cognitive dysfunction publication-title: Neuronal Signal. doi: 10.1042/NS20200101 – volume: 11 start-page: 5319 year: 2019 ident: ref_8 article-title: Association of basal metabolic rate and fuel oxidation in basal conditions and during exercise, with plasma S-klotho: The FIT-AGEING study publication-title: Aging doi: 10.18632/aging.102100 – volume: 31 start-page: 515 year: 2018 ident: ref_15 article-title: Cord blood klotho levels are inversely associated with leptin in healthy Latino neonates at risk for obesity publication-title: J. Pediatr. Endocrinol. Metab. doi: 10.1515/jpem-2017-0357 – volume: 15 start-page: 181 year: 2021 ident: ref_36 article-title: Klotho promoter methylation status and its prognostic value in ovarian cancer publication-title: Mol. Clin. Oncol. doi: 10.3892/mco.2021.2343 – volume: 3 start-page: 183 year: 2018 ident: ref_26 article-title: Klotho, the Key to Healthy Brain Aging? publication-title: Brain Plast. doi: 10.3233/BPL-170057 – volume: 21 start-page: 362 year: 2012 ident: ref_39 article-title: Klotho in health and disease publication-title: Curr. Opin. Nephrol. Hypertens. doi: 10.1097/MNH.0b013e32835422ad – volume: 106 start-page: e2887 year: 2021 ident: ref_16 article-title: Soluble Alpha Klotho in Acromegaly: Comparison with Traditional Markers of Disease Activity publication-title: J. Clin. Endocrinol. Metab. doi: 10.1210/clinem/dgab257 – ident: ref_33 doi: 10.3390/ijms22010043 – volume: 137 start-page: 479 year: 2012 ident: ref_19 article-title: Validation of an Immunoassay for Soluble Klotho Protein: Decreased Levels in Diabetes and Increased Levels in Chronic Kidney Disease publication-title: Am. J. Clin. Pathol. doi: 10.1309/AJCPGPMAF7SFRBO4 – volume: 17 start-page: 1479164120970901 year: 2020 ident: ref_34 article-title: Low levels of circulating anti-ageing hormone Klotho predict the onset and progression of diabetic retinopathy publication-title: Diabetes Vasc. Dis. Res. doi: 10.1177/1479164120970901 – volume: 8 start-page: 950 year: 2018 ident: ref_38 article-title: Protective Effect of Klotho against Ischemic Brain Injury Is Associated with Inhibition of RIG-I/NF-κB Signaling publication-title: Front. Pharmacol. doi: 10.3389/fphar.2017.00950 – ident: ref_3 doi: 10.3390/biom12010070 – volume: 398 start-page: 513 year: 2010 ident: ref_17 article-title: Establishment of sandwich ELISA for soluble alpha-Klotho measurement: Age-dependent change of soluble alpha-Klotho levels in healthy subjects publication-title: Biochem. Biophys. Res. Commun. doi: 10.1016/j.bbrc.2010.06.110 – volume: 12 start-page: 1952 year: 2020 ident: ref_11 article-title: Association between serum levels of Klotho and inflammatory cytokines in cardiovascular disease: A case-control study publication-title: Aging doi: 10.18632/aging.102734 – volume: 10 start-page: 12368 year: 2020 ident: ref_5 article-title: Soluble Klotho, a biomarker and therapeutic strategy to reduce bronchopulmonary dysplasia and pulmonary hypertension in preterm infants publication-title: Sci. Rep. doi: 10.1038/s41598-020-69296-1 – volume: 177 start-page: 4701 year: 2020 ident: ref_1 article-title: Enhanced Klotho availability protects against cardiac dysfunction induced by uraemic cardiomyopathy by regulating Ca2+ handling publication-title: J. Cereb. Blood Flow Metab. – volume: 16 start-page: 3511 year: 2018 ident: ref_27 article-title: Aerobic exercise-stimulated Klotho upregulation extends life span by attenuating the excess production of reactive oxygen species in the brain and kidney publication-title: Exp. Ther. Med. – ident: ref_6 doi: 10.3390/nu12020281 – volume: 28 start-page: 407 year: 2017 ident: ref_25 article-title: Renal Aging: Causes and Consequences publication-title: J. Am. Soc. Nephrol. doi: 10.1681/ASN.2015121308 – volume: 8 start-page: 672000 year: 2021 ident: ref_2 article-title: A Decreased Level of Soluble Klotho Can Predict Cardiovascular Death in No or Mild Abdominal Aortic Calcification Hemodialysis Patients publication-title: Front. Med. doi: 10.3389/fmed.2021.672000 – ident: ref_10 doi: 10.1371/journal.pone.0220796 – volume: 6 start-page: 1849454417733388 year: 2017 ident: ref_31 article-title: The effects of aerobic and anaerobic exercises on circulating soluble-Klotho and IGF-I in young and elderly adults and in CAD patients publication-title: J. Circ. Biomarkers – volume: 10 start-page: 1233 year: 2015 ident: ref_24 article-title: Klotho, stem cells, and aging publication-title: Clin. Interv. Aging doi: 10.2147/CIA.S84978 – volume: 154 start-page: 111518 year: 2021 ident: ref_32 article-title: Association of Klotho with physical performance and frailty in middle-aged and older adults: A systematic review publication-title: Exp. Gerontol. doi: 10.1016/j.exger.2021.111518 – ident: ref_37 doi: 10.1371/journal.pone.0107174 – volume: 20 start-page: 1360 year: 2017 ident: ref_7 article-title: Peripheral Elevation of a Klotho Fragment Enhances Brain Function and Resilience in Young, Aging, and α-Synuclein Transgenic Mice publication-title: Cell Rep. doi: 10.1016/j.celrep.2017.07.024 – volume: 1790 start-page: 1049 year: 2009 ident: ref_4 article-title: Klotho and aging publication-title: Biochim. Biophys. Acta (BBA)-Gen. Subj. doi: 10.1016/j.bbagen.2009.02.005 – volume: 13 start-page: 235 year: 2019 ident: ref_23 article-title: Performance of soluble Klotho assays in clinical samples of kidney disease publication-title: Clin. Kidney J. doi: 10.1093/ckj/sfz085 |
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| Title | References Values of Soluble α-Klotho Serum Levels Using an Enzyme-Linked Immunosorbent Assay in Healthy Adults Aged 18–85 Years |
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