Normal Ligand Binding and Signaling by CD47 (Integrin-associated Protein) Requires a Long Range Disulfide Bond between the Extracellular and Membrane-spanning Domains

• Published in: The Journal of biological chemistry Vol. 276; no. 37; pp. 34607 - 34616
• Main Authors: Rebres, Robert A., Vaz, Louise E., Green, Jennifer M., Brown, Eric J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 14.09.2001; American Society for Biochemistry and Molecular Biology
• Subjects: Amino Acid Sequence; Antigens, CD - chemistry; Antigens, CD - physiology; Antigens, Differentiation; Calcium - metabolism; Carrier Proteins - chemistry; Carrier Proteins - physiology; CD47 Antigen; Cell Membrane - chemistry; Disulfides - chemistry; Humans; Jurkat Cells; Ligands; Membrane Glycoproteins - metabolism; Membrane Microdomains - chemistry; Molecular Sequence Data; Neural Cell Adhesion Molecule L1; Neural Cell Adhesion Molecules - metabolism; Receptors, Antigen, T-Cell - physiology; Receptors, Immunologic; Trypsin - pharmacology

CD47 is a unique member of the Ig superfamily with a single extracellular Ig domain followed by a multiply membrane-spanning (MMS) domain with five transmembrane segments, implicated in both integrin-dependent and -independent signaling cascades. Essentially all functions of CD47 require both the Ig...