Immune-Stimulating Complexes Induce an IL-12-Dependent Cascade of Innate Immune Responses

• Published in: The Journal of immunology (1950) Vol. 162; no. 9; pp. 5536 - 5546
• Main Authors: Smith, Rosemary E, Donachie, Anne M, Grdic, Dubravka, Lycke, Nils, Mowat, Allan McI
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 01.05.1999
• Subjects: Adjuvants, Immunologic - administration & dosage; Animals; Ascitic Fluid - immunology; Ascitic Fluid - pathology; Cell Movement - immunology; Cytokines - biosynthesis; Epitopes - immunology; Female; Immunity, Innate - genetics; Immunophenotyping; Inflammation - immunology; Inflammation - metabolism; Inflammation - pathology; Injections, Intraperitoneal; Interleukin-12 - physiology; ISCOMs - administration & dosage; ISCOMs - immunology; Mice; Mice, Inbred C57BL; Mice, Knockout; Nitric Oxide - biosynthesis; Ovalbumin - administration & dosage; Ovalbumin - immunology; Quillaja Saponins; Reactive Oxygen Species - metabolism; Saponins - administration & dosage; Saponins - immunology

The development of subunit vaccines requires the use of adjuvants that act by stimulating components of the innate immune response. Immune-stimulating complexes (ISCOMS) containing the saponin adjuvant Quil A are potential vaccine vectors that induce a wide range of Ag-specific responses in vivo enc...