Taxonomic assignment of arbuscular mycorrhizal fungi in an 18S metagenomic dataset: a case study with saltcedar (Tamarix aphylla)

• Published in: Mycorrhiza Vol. 30; no. 2-3; pp. 243 - 255
• Main Authors: Stefani, Franck, Bencherif, Karima, Sabourin, Stéphanie, Hadj-Sahraoui, Anissa Lounès, Banchini, Claudia, Séguin, Sylvie, Dalpé, Yolande
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.05.2020; Springer Nature B.V; Springer Verlag
• Subjects: Agriculture; Arbuscular mycorrhizas; Biomedical and Life Sciences; Chemical Sciences; Ecology; Forestry; Fungi; Life Sciences; Metagenomics; Microbiology; Mycorrhizae - genetics; Original Article; Plant Sciences; Symbiosis; Tamaricaceae - genetics; Metagenomics; Arbuscular mycorrhizal fungi; Taxonomic assignment

Many studies describing communities of arbuscular mycorrhizal fungi (AMF, Glomeromycota) based on high-throughput sequencing target the V4 variable region of the 18S ribosomal gene. However, an accurate taxonomic assignment of these short 18S sequences is challenging. Here we describe a simple appro...