Evaluation of Antioxidant and Antimicrobial Activity of Some Plants Collected from Malaysia
Five plant species namely, Phyllanthus acidus, Piper aduncum, Pandanus amaryllifolius, Macaranga peltata and Acacia mangium were analysed for their effective in-vitro bioactivity. The chloroform and aqueous extracted of the selected plants were subjected to TLC bioautography for antioxidant activity...
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Published in | Journal of pure & applied microbiology : an international research journal of microbiology Vol. 13; no. 4; pp. 2363 - 2373 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oriental Scientific Publishing Company
01.12.2019
Journal of Pure and Applied Microbiology |
Subjects | |
Online Access | Get full text |
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Abstract | Five plant species namely, Phyllanthus acidus, Piper aduncum, Pandanus amaryllifolius, Macaranga peltata and Acacia mangium were analysed for their effective in-vitro bioactivity. The chloroform and aqueous extracted of the selected plants were subjected to TLC bioautography for antioxidant activity later all the extracted were subjected for DPPH assay where the chloroform extracts were found to express maximum antioxidant property. Amongst all the plants, Macaranga peltata accounted to 95% DPPH scavenging activity. The antimicrobial studies of the plant extracts were performed via agar well diffusion method, MIC determination, Biofilm inhibition assay in microtitre plate against clinical isolates like Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. It was found that Piper aduncum (chloroform and aqueous extract) and Macaranga peltata (only aqueous) expressed antibacterial activity, of which only chloroform extract of Piper aduncum could show negative influence against the biofilm development of P.aeruginosa. Keywords: DPPH scavenging, antibacterial activity, Staphylococcus aureus and Pseudomonas aeruginosa. |
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AbstractList | Five plant species namely, Phyllanthus acidus, Piper aduncum, Pandanus amaryllifolius, Macaranga peltata and Acacia mangium were analysed for their effective in-vitro bioactivity. The chloroform and aqueous extracted of the selected plants were subjected to TLC bioautography for antioxidant activity later all the extracted were subjected for DPPH assay where the chloroform extracts were found to express maximum antioxidant property. Amongst all the plants, Macaranga peltata accounted to 95% DPPH scavenging activity. The antimicrobial studies of the plant extracts were performed via agar well diffusion method, MIC determination, Biofilm inhibition assay in microtitre plate against clinical isolates like Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. It was found that Piper aduncum (chloroform and aqueous extract) and Macaranga peltata (only aqueous) expressed antibacterial activity, of which only chloroform extract of Piper aduncum could show negative influence against the biofilm development of P.aeruginosa. Keywords: DPPH scavenging, antibacterial activity, Staphylococcus aureus and Pseudomonas aeruginosa. Five plant species namely, Phyllanthus acidus, Piper aduncum, Pandanus amaryllifolius, Macaranga peltata and Acacia mangium were analysed for their effective in-vitro bioactivity. The chloroform and aqueous extracted of the selected plants were subjected to TLC bioautography for antioxidant activity later all the extracted were subjected for DPPH assay where the chloroform extracts were found to express maximum antioxidant property. Amongst all the plants, Macaranga peltata accounted to 95% DPPH scavenging activity. The antimicrobial studies of the plant extracts were performed via agar well diffusion method, MIC determination, Biofilm inhibition assay in microtitre plate against clinical isolates like Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. It was found that Piper aduncum (chloroform and aqueous extract) and Macaranga peltata (only aqueous) expressed antibacterial activity, of which only chloroform extract of Piper aduncum could show negative influence against the biofilm development of P.aeruginosa. |
Audience | Academic |
Author | Pattammadath, Sajeesh Purayil, Sajna Keeyari P., Iyappan Samrot, Antony V Annley, Chew R., Thirumurugan Javad, Pazhayakath Thevarkattil Mohamed P., Raji Ponnaiah, Paulraj A., Jenifer Selvarani |
Author_xml | – sequence: 1 fullname: Purayil, Sajna Keeyari – sequence: 2 fullname: Annley, Chew – sequence: 3 fullname: Ponnaiah, Paulraj – sequence: 4 fullname: Pattammadath, Sajeesh – sequence: 5 fullname: Javad, Pazhayakath Thevarkattil Mohamed – sequence: 6 fullname: A., Jenifer Selvarani – sequence: 7 fullname: P., Raji – sequence: 8 fullname: R., Thirumurugan – sequence: 9 fullname: P., Iyappan – sequence: 10 fullname: Samrot, Antony V |
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CitedBy_id | crossref_primary_10_1016_j_crgsc_2021_100089 crossref_primary_10_1016_j_bcab_2021_102212 crossref_primary_10_1016_j_crgsc_2021_100076 crossref_primary_10_3390_life13030767 |
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Title | Evaluation of Antioxidant and Antimicrobial Activity of Some Plants Collected from Malaysia |
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