Structure-function relationships in an antifreeze polypeptide. The role of charged amino acids

• Published in: The Journal of biological chemistry Vol. 268; no. 22; pp. 16396 - 16400
• Main Authors: Wen, D, Laursen, R A
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Biochemistry and Molecular Biology 05.08.1993
• Subjects: Amino Acid Sequence; Amino Acids - chemistry; Analytical, structural and metabolic biochemistry; Antifreeze Proteins; Biological and medical sciences; Electrochemistry; Freezing; Fundamental and applied biological sciences. Psychology; Glycoproteins - chemistry; Glycoproteins - metabolism; Miscellaneous; Molecular Sequence Data; Proteins; Structure-Activity Relationship; Thermodynamics; Electric charge; Structure activity relation; Antifreeze protein; Analog

Several analogs of an alanine-rich alpha-helical antifreeze polypeptide were synthesized and studied to evaluate the role of charged amino acids on structure and activity. alpha-Helix content and thermal stability were assessed by circular dichroism spectrometry and antifreeze activity by freezing p...