Autodisplay of an avidin with biotin-binding activity on the surface of Escherichia coli

• Published in: Biotechnology letters Vol. 40; no. 3; pp. 591 - 600
• Main Authors: Pardavé-Alejandre, H. D., Alvarado-Yaah, J. E., Pompa-Mera, E. N., Muñoz-Medina, J. E., Sárquiz-Martínez, B., Santacruz-Tinoco, C. E., Manning-Cela, R. G., Ortíz-Navarrete, V., López-Macías, C., González-Bonilla, C. R.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.03.2018; Springer Nature B.V
• Subjects: Amino acids; Applied Microbiology; Avidin; Bacteria; Binding; Biochemistry; Biomedical and Life Sciences; Biotechnology; Biotin; Coliforms; Confocal microscopy; Cytometry; E coli; Escherichia coli; Flow cytometry; Fluorescein; Fluorescence; Fluorescence microscopy; Fusion protein; Gel electrophoresis; Life Sciences; Microbiology; Microscopy; Original Research Paper; Ovalbumin; Proteins; Sodium lauryl sulfate; Autodisplay; surface display; ShdA; Biotin-binding activity; Avidin; In trans surface display

Objectives To display a recombinant avidin fused to the autotransporter ShdA to bind biotinylated molecules on the surface of Escherichia coli . Results Two chimeric protein constructs containing avidin fused to the autotransporter ShdA were expressed on the surface of Escherichia coli DH5α. One fus...