Fiber alignment in 3D collagen networks as a biophysical marker for cell contractility

• Published in: Matrix biology Vol. 124; pp. 39 - 48
• Main Authors: Böhringer, David, Bauer, Andreas, Moravec, Ivana, Bischof, Lars, Kah, Delf, Mark, Christoph, Grundy, Thomas J, Görlach, Ekkehard, O'Neill, Geraldine M, Budday, Silvia, Strissel, Pamela L, Strick, Reiner, Malandrino, Andrea, Gerum, Richard, Mak, Michael, Rausch, Martin, Fabry, Ben
• Format: Journal Article
• Language: English
• Published: Netherlands 01.12.2023
• Subjects: Cell Line; Collagen - metabolism; Extracellular Matrix - metabolism; Fiber Orientation; Biopolymer Networks; Mechanobiology; Traction Force Microscopy

Cells cultured in 3D fibrous biopolymer matrices exert traction forces on their environment that induce deformations and remodeling of the fiber network. By measuring these deformations, the traction forces can be reconstructed if the mechanical properties of the matrix and the force-free matrix con...