Structural Insight into the Gene Expression Profiling of the hcn Operon in Pseudomonas aeruginosa
Pseudomonas aeruginosa is a common opportunistic human pathogen. It generally attacks immunosuppressed patients like AIDS, cancer, cystic fibrosis, etc. The virulence of P. aeruginosa is mediated by various virulence factors. One of such potential virulence factors is HCN synthesized by HCN synthase...
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Published in | Applied biochemistry and biotechnology Vol. 182; no. 3; pp. 1144 - 1157 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.07.2017
Springer Nature B.V |
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Abstract | Pseudomonas aeruginosa
is a common opportunistic human pathogen. It generally attacks immunosuppressed patients like AIDS, cancer, cystic fibrosis, etc. The virulence of
P. aeruginosa
is mediated by various virulence factors. One of such potential virulence factors is HCN synthesized by HCN synthase enzyme, which is encoded by the
hcnABC
operon. The expressions of the genes of this operon are regulated by three transcriptional regulators, viz., LasR, ANR, and RhlR. In our previous work, we analyzed the molecular details of the functionalities of LasR. In this work, we focused on ANR. ANR is a regulatory protein which belongs to the FNR family and works in anaerobic condition. ANR binds to the promoter DNA, named ANR box, as a dimer. The dimerization of this ANR protein is regulated by Fe
4
S
4
, an iron-sulfur cluster. This dimer of ANR (ANR-Fe
4
S
4
/ANR-Fe
4
S
4
) recognizes and binds the promoter DNA sequence and regulates the transcription of this
hcnABC
operon. Till date, the biomolecular details of the interactions of ANR dimer with the promoter DNA are not fully understood. Thus, we built the molecular model of ANR-Fe
4
S
4
/ANR-Fe
4
S
4
. We docked the complex with the corresponding promoter DNA region. We analyzed the mode of interactions with the promoter DNA under different conditions. Thus, we tried to analyze the functionality of the ANR protein during the expressions of the genes of the
hcnABC
operon. So far, this is the first report to detail the molecular mechanism of the gene expression in
P. aeruginosa
. |
---|---|
AbstractList | Pseudomonas aeruginosa is a common opportunistic human pathogen. It generally attacks immunosuppressed patients like AIDS, cancer, cystic fibrosis, etc. The virulence of P. aeruginosa is mediated by various virulence factors. One of such potential virulence factors is HCN synthesized by HCN synthase enzyme, which is encoded by the hcnABC operon. The expressions of the genes of this operon are regulated by three transcriptional regulators, viz., LasR, ANR, and RhlR. In our previous work, we analyzed the molecular details of the functionalities of LasR. In this work, we focused on ANR. ANR is a regulatory protein which belongs to the FNR family and works in anaerobic condition. ANR binds to the promoter DNA, named ANR box, as a dimer. The dimerization of this ANR protein is regulated by Fe4S4, an iron-sulfur cluster. This dimer of ANR (ANR-Fe4S4/ANR-Fe4S4) recognizes and binds the promoter DNA sequence and regulates the transcription of this hcnABC operon. Till date, the biomolecular details of the interactions of ANR dimer with the promoter DNA are not fully understood. Thus, we built the molecular model of ANR-Fe4S4/ANR-Fe4S4. We docked the complex with the corresponding promoter DNA region. We analyzed the mode of interactions with the promoter DNA under different conditions. Thus, we tried to analyze the functionality of the ANR protein during the expressions of the genes of the hcnABC operon. So far, this is the first report to detail the molecular mechanism of the gene expression in P. aeruginosa. Pseudomonas aeruginosa is a common opportunistic human pathogen. It generally attacks immunosuppressed patients like AIDS, cancer, cystic fibrosis, etc. The virulence of P. aeruginosa is mediated by various virulence factors. One of such potential virulence factors is HCN synthesized by HCN synthase enzyme, which is encoded by the hcnABC operon. The expressions of the genes of this operon are regulated by three transcriptional regulators, viz., LasR, ANR, and RhlR. In our previous work, we analyzed the molecular details of the functionalities of LasR. In this work, we focused on ANR. ANR is a regulatory protein which belongs to the FNR family and works in anaerobic condition. ANR binds to the promoter DNA, named ANR box, as a dimer. The dimerization of this ANR protein is regulated by Fe S , an iron-sulfur cluster. This dimer of ANR (ANR-Fe S /ANR-Fe S ) recognizes and binds the promoter DNA sequence and regulates the transcription of this hcnABC operon. Till date, the biomolecular details of the interactions of ANR dimer with the promoter DNA are not fully understood. Thus, we built the molecular model of ANR-Fe S /ANR-Fe S . We docked the complex with the corresponding promoter DNA region. We analyzed the mode of interactions with the promoter DNA under different conditions. Thus, we tried to analyze the functionality of the ANR protein during the expressions of the genes of the hcnABC operon. So far, this is the first report to detail the molecular mechanism of the gene expression in P. aeruginosa. Pseudomonas aeruginosa is a common opportunistic human pathogen. It generally attacks immunosuppressed patients like AIDS, cancer, cystic fibrosis, etc. The virulence of P. aeruginosa is mediated by various virulence factors. One of such potential virulence factors is HCN synthesized by HCN synthase enzyme, which is encoded by the hcnABC operon. The expressions of the genes of this operon are regulated by three transcriptional regulators, viz., LasR, ANR, and RhlR. In our previous work, we analyzed the molecular details of the functionalities of LasR. In this work, we focused on ANR. ANR is a regulatory protein which belongs to the FNR family and works in anaerobic condition. ANR binds to the promoter DNA, named ANR box, as a dimer. The dimerization of this ANR protein is regulated by Fe 4 S 4 , an iron-sulfur cluster. This dimer of ANR (ANR-Fe 4 S 4 /ANR-Fe 4 S 4 ) recognizes and binds the promoter DNA sequence and regulates the transcription of this hcnABC operon. Till date, the biomolecular details of the interactions of ANR dimer with the promoter DNA are not fully understood. Thus, we built the molecular model of ANR-Fe 4 S 4 /ANR-Fe 4 S 4 . We docked the complex with the corresponding promoter DNA region. We analyzed the mode of interactions with the promoter DNA under different conditions. Thus, we tried to analyze the functionality of the ANR protein during the expressions of the genes of the hcnABC operon. So far, this is the first report to detail the molecular mechanism of the gene expression in P. aeruginosa . |
Author | Chowdhury, Nilkanta Bagchi, Angshuman |
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CitedBy_id | crossref_primary_10_1016_j_vaccine_2018_08_070 crossref_primary_10_1093_jxb_erz225 crossref_primary_10_1039_C7ME00083A crossref_primary_10_1128_mBio_01362_19 crossref_primary_10_1002_jmr_2738 |
Cites_doi | 10.1002/bip.360221211 10.1093/bioinformatics/btu097 10.1093/nar/gki481 10.1128/JB.182.24.6940-6949.2000 10.1093/clinids/5.2.279 10.1016/j.gene.2015.12.067 10.1093/nar/gkm290 10.1002/prot.340040106 10.1107/S0021889892009944 10.1128/JB.186.20.6837-6844.2004 10.1093/nar/28.1.235 10.1002/jcc.20566 10.1002/jcc.540040211 10.1074/jbc.M106569200 10.1016/S0022-2836(05)80360-2 10.1128/JB.183.21.6207-6214.2001 10.1016/S0076-6879(97)77022-8 10.1126/sciadv.1501086 10.3201/eid0404.980405 |
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Snippet | Pseudomonas aeruginosa
is a common opportunistic human pathogen. It generally attacks immunosuppressed patients like AIDS, cancer, cystic fibrosis, etc. The... Pseudomonas aeruginosa is a common opportunistic human pathogen. It generally attacks immunosuppressed patients like AIDS, cancer, cystic fibrosis, etc. The... |
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SubjectTerms | Acquired immune deficiency syndrome AIDS Anaerobic conditions ANR protein Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Biochemistry Biotechnology Cancer Chemistry Chemistry and Materials Science Clusters Coding Construction Cystic fibrosis Deoxyribonucleic acid Dimerization DNA Gene expression Gene Expression Profiling Gene Expression Regulation, Bacterial - physiology Genes Humans Iron Mathematical models Nucleotide sequence Operon - physiology Opportunist infection Pathogens Patients Promoter Regions, Genetic - physiology Proteins Pseudomonas Pseudomonas aeruginosa Pseudomonas aeruginosa - genetics Pseudomonas aeruginosa - metabolism Regulators RhlR protein Sulfur Trans-Activators - genetics Trans-Activators - metabolism Transcription Transcription, Genetic - physiology Virulence Virulence factors Yeast |
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Title | Structural Insight into the Gene Expression Profiling of the hcn Operon in Pseudomonas aeruginosa |
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