Structural Requirements for Interactions between Leucine-sorting Signals and Clathrin-associated Adaptor Protein Complex AP3

• Published in: The Journal of biological chemistry Vol. 277; no. 49; pp. 47436 - 47443
• Main Authors: Rodionov, Dmitrii G., Höning, Stefan, Silye, Aleksandra, Kongsvik, Thomas L., von Figura, Kurt, Bakke, Oddmund
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 06.12.2002; American Society for Biochemistry and Molecular Biology
• Subjects: Adaptor Protein Complex 1 - metabolism; Adaptor Protein Complex 2 - metabolism; Adaptor Protein Complex 3 - chemistry; Adaptor Protein Complex 3 - metabolism; Amino Acid Sequence; Animals; Cell Line; Clathrin - metabolism; DNA - metabolism; Dogs; Glutathione Transferase - metabolism; Kinetics; Leucine - chemistry; Leucine - metabolism; Microscopy, Fluorescence; Molecular Sequence Data; Mutation; Precipitin Tests; Protein Binding; Recombinant Fusion Proteins - metabolism; Surface Plasmon Resonance; Transfection

Cytoplasmic tails of LIMPII and the invariant chain contain similar leucine-based sorting signals, but the invariant chain interacts only with AP1 and AP2, whereas LIMPII interacts strongly with AP3. In a series of in vitro experiments, we investigated the effect of residues upstream of the leucine...