The Antisense Approach in Amyloid Light Chain Amyloidosis: Identification of Monoclonal Ig and Inhibition of Its Production by Antisense Oligonucleotides in In Vitro and In Vivo Models

• Published in: The Journal of immunology (1950) Vol. 169; no. 7; pp. 4039 - 4045
• Main Authors: Ohno, Satoko, Yoshimoto, Mitsuru, Honda, Saho, Miyachi, Sae, Ishida, Tadao, Itoh, Fumio, Endo, Takao, Chiba, Susumu, Imai, Kohzoh
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 01.10.2002
• Subjects: Amino Acid Sequence; Amyloid - immunology; Amyloidosis - genetics; Amyloidosis - immunology; Amyloidosis - therapy; Animals; Antibodies, Monoclonal - analysis; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - blood; Antibodies, Monoclonal - genetics; Base Sequence; Gene Amplification; Humans; Immunoglobulin lambda-Chains - analysis; Immunoglobulin lambda-Chains - biosynthesis; Immunoglobulin lambda-Chains - blood; Immunoglobulin lambda-Chains - genetics; Immunoglobulin Variable Region - biosynthesis; Immunoglobulin Variable Region - genetics; Immunosuppressive Agents - administration & dosage; Immunosuppressive Agents - pharmacology; Injections, Intralesional; Mice; Mice, SCID; Molecular Sequence Data; Multiple Myeloma - genetics; Multiple Myeloma - immunology; Multiple Myeloma - therapy; Neoplasm Transplantation; Oligonucleotides, Antisense - administration & dosage; Oligonucleotides, Antisense - pharmacology; Paraproteinemias - genetics; Paraproteinemias - immunology; Paraproteinemias - therapy; RNA, Messenger - metabolism; Transplantation, Heterologous; Tumor Cells, Cultured - transplantation

Primary amyloid L chain (AL) amyloidosis is a plasma cell disorder in which depositions of AL cause progressive organ failure. The lack of effective therapies for this fatal disease prompts exploration of newer treatment avenues. We have investigated the application of antisense oligonucleotides (AS...