Expression of vitamin D 1α-hydroxylase in human gingival fibroblasts in vivo
Vitamin D 1α-hydroxylase CYP27B1 is the key factor in the vitamin D pathway. Previously, we analyzed the expression of CYP27B1 in human gingival fibroblasts in vitro. In the present study, we analyzed the gingival expression of CYP27B1 in vivo. Forty-two patients with periodontitis Stage IV Grade C...
Saved in:
| Published in | PeerJ (San Francisco, CA) Vol. 9; p. e10279 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
PeerJ, Inc
04.01.2021
PeerJ Inc |
| Subjects | |
| Online Access | Get full text |
| ISSN | 2167-8359 2167-8359 |
| DOI | 10.7717/peerj.10279 |
Cover
| Abstract | Vitamin D 1α-hydroxylase CYP27B1 is the key factor in the vitamin D pathway. Previously, we analyzed the expression of CYP27B1 in human gingival fibroblasts in vitro. In the present study, we analyzed the gingival expression of CYP27B1 in vivo.
Forty-two patients with periodontitis Stage IV Grade C and 33 controls were recruited. All patients with periodontitis had unsalvageable teeth and part of the wall of the periodontal pocket was resected and obtained after tooth extraction. All controls needed crown-lengthening surgery, and samples of gingiva resected during surgery were also harvested. All the individuals' gingivae were used for immunohistochemistry and immunofluorescence. In addition, gingivae from seventeen subjects of the diseased group and twelve subjects of the control group were analyzed by real-time PCR.
Expression of CYP27B1 was detected both in gingival epithelia and in gingival connective tissues, and the expression in connective tissues colocalized with vimentin, indicating that CYP27B1 protein is expressed in gingival fibroblasts. The expression of CYP27B1 mRNA in gingival connective tissues and the CYP27B1 staining scores in gingival fibroblasts in the diseased group were significantly higher than those in the control group.
Expression of CYP27B1 in human gingival tissues was detected, not only in the fibroblasts of gingival connective tissues, but also in the gingival epithelial cells, and might be positively correlated with periodontal inflammation. |
|---|---|
| AbstractList | Background Vitamin D 1α-hydroxylase CYP27B1 is the key factor in the vitamin D pathway. Previously, we analyzed the expression of CYP27B1 in human gingival fibroblasts in vitro. In the present study, we analyzed the gingival expression of CYP27B1 in vivo. Methods Forty-two patients with periodontitis Stage IV Grade C and 33 controls were recruited. All patients with periodontitis had unsalvageable teeth and part of the wall of the periodontal pocket was resected and obtained after tooth extraction. All controls needed crown-lengthening surgery, and samples of gingiva resected during surgery were also harvested. All the individuals’ gingivae were used for immunohistochemistry and immunofluorescence. In addition, gingivae from seventeen subjects of the diseased group and twelve subjects of the control group were analyzed by real-time PCR. Results Expression of CYP27B1 was detected both in gingival epithelia and in gingival connective tissues, and the expression in connective tissues colocalized with vimentin, indicating that CYP27B1 protein is expressed in gingival fibroblasts. The expression of CYP27B1 mRNA in gingival connective tissues and the CYP27B1 staining scores in gingival fibroblasts in the diseased group were significantly higher than those in the control group. Conclusions Expression of CYP27B1 in human gingival tissues was detected, not only in the fibroblasts of gingival connective tissues, but also in the gingival epithelial cells, and might be positively correlated with periodontal inflammation. Vitamin D 1α-hydroxylase CYP27B1 is the key factor in the vitamin D pathway. Previously, we analyzed the expression of CYP27B1 in human gingival fibroblasts in vitro. In the present study, we analyzed the gingival expression of CYP27B1 in vivo. Forty-two patients with periodontitis Stage IV Grade C and 33 controls were recruited. All patients with periodontitis had unsalvageable teeth and part of the wall of the periodontal pocket was resected and obtained after tooth extraction. All controls needed crown-lengthening surgery, and samples of gingiva resected during surgery were also harvested. All the individuals' gingivae were used for immunohistochemistry and immunofluorescence. In addition, gingivae from seventeen subjects of the diseased group and twelve subjects of the control group were analyzed by real-time PCR. Expression of CYP27B1 was detected both in gingival epithelia and in gingival connective tissues, and the expression in connective tissues colocalized with vimentin, indicating that CYP27B1 protein is expressed in gingival fibroblasts. The expression of CYP27B1 mRNA in gingival connective tissues and the CYP27B1 staining scores in gingival fibroblasts in the diseased group were significantly higher than those in the control group. Expression of CYP27B1 in human gingival tissues was detected, not only in the fibroblasts of gingival connective tissues, but also in the gingival epithelial cells, and might be positively correlated with periodontal inflammation. Vitamin D 1α-hydroxylase CYP27B1 is the key factor in the vitamin D pathway. Previously, we analyzed the expression of CYP27B1 in human gingival fibroblasts in vitro. In the present study, we analyzed the gingival expression of CYP27B1 in vivo.BACKGROUNDVitamin D 1α-hydroxylase CYP27B1 is the key factor in the vitamin D pathway. Previously, we analyzed the expression of CYP27B1 in human gingival fibroblasts in vitro. In the present study, we analyzed the gingival expression of CYP27B1 in vivo.Forty-two patients with periodontitis Stage IV Grade C and 33 controls were recruited. All patients with periodontitis had unsalvageable teeth and part of the wall of the periodontal pocket was resected and obtained after tooth extraction. All controls needed crown-lengthening surgery, and samples of gingiva resected during surgery were also harvested. All the individuals' gingivae were used for immunohistochemistry and immunofluorescence. In addition, gingivae from seventeen subjects of the diseased group and twelve subjects of the control group were analyzed by real-time PCR.METHODSForty-two patients with periodontitis Stage IV Grade C and 33 controls were recruited. All patients with periodontitis had unsalvageable teeth and part of the wall of the periodontal pocket was resected and obtained after tooth extraction. All controls needed crown-lengthening surgery, and samples of gingiva resected during surgery were also harvested. All the individuals' gingivae were used for immunohistochemistry and immunofluorescence. In addition, gingivae from seventeen subjects of the diseased group and twelve subjects of the control group were analyzed by real-time PCR.Expression of CYP27B1 was detected both in gingival epithelia and in gingival connective tissues, and the expression in connective tissues colocalized with vimentin, indicating that CYP27B1 protein is expressed in gingival fibroblasts. The expression of CYP27B1 mRNA in gingival connective tissues and the CYP27B1 staining scores in gingival fibroblasts in the diseased group were significantly higher than those in the control group.RESULTSExpression of CYP27B1 was detected both in gingival epithelia and in gingival connective tissues, and the expression in connective tissues colocalized with vimentin, indicating that CYP27B1 protein is expressed in gingival fibroblasts. The expression of CYP27B1 mRNA in gingival connective tissues and the CYP27B1 staining scores in gingival fibroblasts in the diseased group were significantly higher than those in the control group.Expression of CYP27B1 in human gingival tissues was detected, not only in the fibroblasts of gingival connective tissues, but also in the gingival epithelial cells, and might be positively correlated with periodontal inflammation.CONCLUSIONSExpression of CYP27B1 in human gingival tissues was detected, not only in the fibroblasts of gingival connective tissues, but also in the gingival epithelial cells, and might be positively correlated with periodontal inflammation. |
| ArticleNumber | e10279 |
| Author | Su, Jing Liu, Kaining Hou, Jianxia Meng, Huanxin Zhang, Jianyun Han, Bing |
| Author_xml | – sequence: 1 givenname: Kaining surname: Liu fullname: Liu, Kaining organization: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China, National Clinical Research Center for Oral Diseases, Beijing, China, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing, China, Beijing Key Laboratory of Digital Stomatology, Beijing, China – sequence: 2 givenname: Bing surname: Han fullname: Han, Bing organization: National Clinical Research Center for Oral Diseases, Beijing, China, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing, China, Beijing Key Laboratory of Digital Stomatology, Beijing, China, Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology, Beijing, China – sequence: 3 givenname: Jianxia surname: Hou fullname: Hou, Jianxia organization: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China, National Clinical Research Center for Oral Diseases, Beijing, China, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing, China, Beijing Key Laboratory of Digital Stomatology, Beijing, China – sequence: 4 givenname: Jianyun surname: Zhang fullname: Zhang, Jianyun organization: National Clinical Research Center for Oral Diseases, Beijing, China, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing, China, Beijing Key Laboratory of Digital Stomatology, Beijing, China, Department of Oral Pathology, Peking University School and Hospital of Stomatology, Beijing, China – sequence: 5 givenname: Jing surname: Su fullname: Su, Jing organization: Department of Pathology, Peking University Health Science Center, Beijing, China – sequence: 6 givenname: Huanxin surname: Meng fullname: Meng, Huanxin organization: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China, National Clinical Research Center for Oral Diseases, Beijing, China, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing, China, Beijing Key Laboratory of Digital Stomatology, Beijing, China |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/33505780$$D View this record in MEDLINE/PubMed |
| BookMark | eNptkctKxDAUhoMoXkZX7qXgRpCOSdvcNoJ4B8WNrkPaJjMZ2mRM2jLzWL6Iz2TGGyqeTULOd37-_GcHrFtnFQD7CI4pRfRkrpSfjRHMKF8D2xkiNGU55us_7ltgL4QZjMUyAlm-CbbyHENMGdwG95eLuVchGGcTp5PBdLI1NrlI0OtLOl3W3i2WjQwqiY_TvpU2mRg7MYNsEm1K78rY7MKqO5jB7YINLZug9j7PEXi6unw8v0nvHq5vz8_u0qqAvEuRrBBDNSea5ljDIuOaKFQjjmudEZxRVReIYJIjzStIpapKDSnWrCQoAjQfgdMP3XlftqqulO28bMTcm1b6pXDSiN8da6Zi4gZBKeOM5FHg6FPAu-dehU60JlSqaaRVrg8iK2JWsVgW0cM_6Mz13sbvRYoWnEGMVoIHPx19W_lKOgLoA6i8C8ErLaqYdRdzjwZNIxAUq4WK94WK94XGmeM_M1-y_9Fv1JSjFQ |
| CitedBy_id | crossref_primary_10_1016_j_identj_2023_06_004 crossref_primary_10_1111_odi_15147 crossref_primary_10_1111_jre_12986 crossref_primary_10_3390_biom12060833 crossref_primary_10_3177_jnsv_69_90 |
| Cites_doi | 10.1126/science.1123933 10.1902/jop.2015.140262 10.1126/science.277.5333.1827 10.1371/journal.pone.0039878 10.1111/jre.12445 10.1681/ASN.V133621 10.1006/meth.2001.1262 10.1902/jop.2009.090355 10.1902/jop.2009.080675 10.1038/s41598-019-45161-8 10.1093/ajcn/80.1.108 10.1128/IAI.00099-11 10.1172/JCI30142 10.1016/j.bbamem.2015.11.003 10.1111/jcpe.12936 10.1161/01.CIR.0000160353.27927.70 10.1111/j.1600-051x.1981.tb02031.x 10.1371/journal.pone.0052053 10.3390/ijms19092663 10.2220/biomedres.37.199 10.3177/jnsv.64.307 10.1167/iovs.13-13019 10.1126/science.1233665 10.1177/0022034514534985 10.1111/jre.12137 10.1038/s41574-019-0312-5 10.1002/JPER.17-0530 10.1096/fj.06-6374fje 10.1016/j.humpath.2010.06.010 10.1038/s41585-018-0015-z 10.1111/jre.12646 10.1152/physrev.00021.2018 10.1111/jcpe.12946 10.1007/978-981-13-3588-4_12 10.1017/S1368980013000177 10.3390/nu10111656 10.1111/jcpe.12945 |
| ContentType | Journal Article |
| Copyright | 2021 Liu et al. 2021 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2021 Liu et al. 2021 Liu et al. |
| Copyright_xml | – notice: 2021 Liu et al. – notice: 2021 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2021 Liu et al. 2021 Liu et al. |
| DBID | AAYXX CITATION NPM 3V. 7XB 88I 8FE 8FH 8FK ABUWG AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO GNUQQ HCIFZ LK8 M2P M7P PHGZM PHGZT PIMPY PKEHL PQEST PQGLB PQQKQ PQUKI PRINS Q9U 7X8 5PM |
| DOI | 10.7717/peerj.10279 |
| DatabaseName | CrossRef PubMed ProQuest Central (Corporate) ProQuest Central (purchase pre-March 2016) Science Database (Alumni Edition) ProQuest SciTech Collection ProQuest Natural Science Collection ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest Central UK/Ireland ProQuest Central Essentials Biological Science Collection ProQuest Central Database Suite (ProQuest) Natural Science Collection (ProQuest) ProQuest One Community College ProQuest Central Korea ProQuest Central Student SciTech Collection (ProQuest) Biological Sciences Science Database Biological Science Database ProQuest Central Premium ProQuest One Academic (New) Publicly Available Content Database ProQuest One Academic Middle East (New) ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China ProQuest Central Basic MEDLINE - Academic PubMed Central (Full Participant titles) |
| DatabaseTitle | CrossRef PubMed Publicly Available Content Database ProQuest Central Student ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest Natural Science Collection ProQuest Central China ProQuest Central ProQuest One Applied & Life Sciences Natural Science Collection ProQuest Central Korea Biological Science Collection ProQuest Central (New) ProQuest Science Journals (Alumni Edition) ProQuest Biological Science Collection ProQuest Central Basic ProQuest Science Journals ProQuest One Academic Eastern Edition Biological Science Database ProQuest SciTech Collection ProQuest One Academic UKI Edition ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | Publicly Available Content Database PubMed MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: BENPR name: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine Dentistry |
| EISSN | 2167-8359 |
| ExternalDocumentID | PMC7789863 33505780 10_7717_peerj_10279 |
| Genre | Journal Article |
| GeographicLocations | Beijing China United States--US China |
| GeographicLocations_xml | – name: China – name: Beijing China – name: United States--US |
| GrantInformation_xml | – fundername: National Natural Science Foundations of China grantid: 81100749; 81271149 |
| GroupedDBID | 53G 5VS 88I 8FE 8FH AAFWJ AAYXX ABUWG ADBBV ADRAZ AENEX AFKRA AFPKN ALMA_UNASSIGNED_HOLDINGS AOIJS AZQEC BAWUL BBNVY BCNDV BENPR BHPHI BPHCQ CCPQU CITATION DIK DWQXO ECGQY GNUQQ GROUPED_DOAJ GX1 H13 HCIFZ HYE IAO IEA IHR IHW ITC KQ8 LK8 M2P M48 M7P M~E OK1 PHGZM PHGZT PIMPY PQQKQ PROAC RPM W2D YAO NPM PQGLB 3V. 7XB 8FK PKEHL PQEST PQUKI PRINS Q9U 7X8 PUEGO 5PM |
| ID | FETCH-LOGICAL-c409t-1ac181d96f735f0429f6e1d195df26527ed4165631f9c07aecbf075f8b61f2673 |
| IEDL.DBID | M48 |
| ISSN | 2167-8359 |
| IngestDate | Thu Aug 21 14:10:09 EDT 2025 Thu Sep 04 17:53:28 EDT 2025 Fri Jul 25 11:46:11 EDT 2025 Mon Jul 21 05:28:16 EDT 2025 Tue Jul 01 01:10:57 EDT 2025 Thu Apr 24 23:10:30 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Keywords | 25-hydroxyvitamin D3 1-alpha-hydroxylase Periodontitis Gene expression Gingiva |
| Language | English |
| License | https://creativecommons.org/licenses/by/4.0 2021 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c409t-1ac181d96f735f0429f6e1d195df26527ed4165631f9c07aecbf075f8b61f2673 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
| OpenAccessLink | http://journals.scholarsportal.info/openUrl.xqy?doi=10.7717/peerj.10279 |
| PMID | 33505780 |
| PQID | 2474980513 |
| PQPubID | 2045935 |
| ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_7789863 proquest_miscellaneous_2482666682 proquest_journals_2474980513 pubmed_primary_33505780 crossref_citationtrail_10_7717_peerj_10279 crossref_primary_10_7717_peerj_10279 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2021-01-04 |
| PublicationDateYYYYMMDD | 2021-01-04 |
| PublicationDate_xml | – month: 01 year: 2021 text: 2021-01-04 day: 04 |
| PublicationDecade | 2020 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States – name: San Diego – name: San Diego, USA |
| PublicationTitle | PeerJ (San Francisco, CA) |
| PublicationTitleAlternate | PeerJ |
| PublicationYear | 2021 |
| Publisher | PeerJ, Inc PeerJ Inc |
| Publisher_xml | – name: PeerJ, Inc – name: PeerJ Inc |
| References | Alsalem (10.7717/peerj.10279/ref-1) 2014; 55 Piera-Velazquez (10.7717/peerj.10279/ref-23) 2019; 99 Dietrich (10.7717/peerj.10279/ref-5) 2004; 80 Xhindoli (10.7717/peerj.10279/ref-33) 2016; 1858 Zehnder (10.7717/peerj.10279/ref-35) 2002; 13 Zhan (10.7717/peerj.10279/ref-36) 2014; 93 Li (10.7717/peerj.10279/ref-9) 2017; 52 Livak (10.7717/peerj.10279/ref-15) 2001; 25 Bikle (10.7717/peerj.10279/ref-3) 2020; 16 Teles (10.7717/peerj.10279/ref-29) 2013; 339 Tonetti (10.7717/peerj.10279/ref-30) 2018; 45 Liu (10.7717/peerj.10279/ref-10) 2012; 7 Liu (10.7717/peerj.10279/ref-14) 2006; 311 Medrano (10.7717/peerj.10279/ref-20) 2018; 19 Antonoglou (10.7717/peerj.10279/ref-2) 2015; 86 Lang (10.7717/peerj.10279/ref-8) 2018; 45 Markiewicz (10.7717/peerj.10279/ref-17) 2019; 9 Capiod (10.7717/peerj.10279/ref-4) 2018; 15 Menzel (10.7717/peerj.10279/ref-21) 2019; 54 Sassi (10.7717/peerj.10279/ref-24) 2018; 10 Jimenez (10.7717/peerj.10279/ref-7) 2014; 17 Tada (10.7717/peerj.10279/ref-27) 2016; 37 Mazza (10.7717/peerj.10279/ref-18) 1981; 8 Liu (10.7717/peerj.10279/ref-12) 2010; 81 Wang (10.7717/peerj.10279/ref-32) 2019; 1117 Yoon (10.7717/peerj.10279/ref-34) 2010; 41 Papapanou (10.7717/peerj.10279/ref-22) 2018; 45 Zhou (10.7717/peerj.10279/ref-37) 2018; 64 Gao (10.7717/peerj.10279/ref-6) 2018; 89 Takeyama (10.7717/peerj.10279/ref-28) 1997; 277 Van Driel (10.7717/peerj.10279/ref-31) 2006; 20 McMahon (10.7717/peerj.10279/ref-19) 2011; 79 Somjen (10.7717/peerj.10279/ref-26) 2005; 111 Lu (10.7717/peerj.10279/ref-16) 2014; 49 Liu (10.7717/peerj.10279/ref-11) 2012; 7 Schauber (10.7717/peerj.10279/ref-25) 2007; 117 Liu (10.7717/peerj.10279/ref-13) 2009; 80 |
| References_xml | – volume: 311 start-page: 1770 year: 2006 ident: 10.7717/peerj.10279/ref-14 article-title: Toll-like receptor triggering of a vitamin D-mediated human antimicrobial response publication-title: Science doi: 10.1126/science.1123933 – volume: 86 start-page: 755 year: 2015 ident: 10.7717/peerj.10279/ref-2 article-title: Associations between serum 25-hydroxyvitamin d and periodontal pocketing and gingival bleeding: results of a study in a non-smoking population in Finland publication-title: Journal of Periodontology doi: 10.1902/jop.2015.140262 – volume: 277 start-page: 1827 year: 1997 ident: 10.7717/peerj.10279/ref-28 article-title: 25-Hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis publication-title: Science doi: 10.1126/science.277.5333.1827 – volume: 7 start-page: e39878 year: 2012 ident: 10.7717/peerj.10279/ref-11 article-title: Characterization of the autocrine/paracrine function of vitamin d in human gingival fibroblasts and periodontal ligament cells publication-title: PLOS One doi: 10.1371/journal.pone.0039878 – volume: 52 start-page: 755 year: 2017 ident: 10.7717/peerj.10279/ref-9 article-title: Vitamin D-binding protein expression in healthy tooth and periodontium: an experimental study both in monkeys in vivo and in humans in vitro publication-title: Journal of Periodontal Research doi: 10.1111/jre.12445 – volume: 13 start-page: 621 year: 2002 ident: 10.7717/peerj.10279/ref-35 article-title: Synthesis of 1, 25-dihydroxyvitamin D(3) by human endothelial cells is regulated by inflammatory cytokines: a novel autocrine determinant of vascular cell adhesion publication-title: Journal of the American Society of Nephrology doi: 10.1681/ASN.V133621 – volume: 25 start-page: 402 year: 2001 ident: 10.7717/peerj.10279/ref-15 article-title: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method publication-title: Methods doi: 10.1006/meth.2001.1262 – volume: 81 start-page: 260 year: 2010 ident: 10.7717/peerj.10279/ref-12 article-title: Initial periodontal therapy reduced systemic and local 25-hydroxy vitamin D(3) and interleukin-1beta in patients with aggressive periodontitis publication-title: Journal of Periodontology doi: 10.1902/jop.2009.090355 – volume: 80 start-page: 1114 year: 2009 ident: 10.7717/peerj.10279/ref-13 article-title: Elevated plasma calcifediol is associated with aggressive periodontitis publication-title: Journal of Periodontology doi: 10.1902/jop.2009.080675 – volume: 9 start-page: 9142 year: 2019 ident: 10.7717/peerj.10279/ref-17 article-title: Vitamin D receptors (VDR), hydroxylases CYP27B1 and CYP24A1 and retinoid-related orphan receptors (ROR) level in human uveal tract and ocular melanoma with different melanization levels publication-title: Scientific Reports doi: 10.1038/s41598-019-45161-8 – volume: 80 start-page: 108 year: 2004 ident: 10.7717/peerj.10279/ref-5 article-title: Association between serum concentrations of 25-hydroxyvitamin D3 and periodontal disease in the US population publication-title: American Journal of Clinical Nutrition doi: 10.1093/ajcn/80.1.108 – volume: 79 start-page: 2250 year: 2011 ident: 10.7717/peerj.10279/ref-19 article-title: Vitamin D-mediated induction of innate immunity in gingival epithelial cells publication-title: Infection and Immunity doi: 10.1128/IAI.00099-11 – volume: 117 start-page: 803 year: 2007 ident: 10.7717/peerj.10279/ref-25 article-title: Injury enhances TLR2 function and antimicrobial peptide expression through a vitamin D-dependent mechanism publication-title: Journal of Clinical Investigation doi: 10.1172/JCI30142 – volume: 1858 start-page: 546 year: 2016 ident: 10.7717/peerj.10279/ref-33 article-title: The human cathelicidin LL-37–A pore-forming antibacterial peptide and host-cell modulator publication-title: Biochimica et Biophysica Acta doi: 10.1016/j.bbamem.2015.11.003 – volume: 45 start-page: S9 issue: Suppl 20 year: 2018 ident: 10.7717/peerj.10279/ref-8 article-title: Periodontal health publication-title: Journal of Clinical Periodontology doi: 10.1111/jcpe.12936 – volume: 111 start-page: 1666 year: 2005 ident: 10.7717/peerj.10279/ref-26 article-title: 25-hydroxyvitamin D3-1alpha-hydroxylase is expressed in human vascular smooth muscle cells and is upregulated by parathyroid hormone and estrogenic compounds publication-title: Circulation doi: 10.1161/01.CIR.0000160353.27927.70 – volume: 8 start-page: 203 year: 1981 ident: 10.7717/peerj.10279/ref-18 article-title: Clinical and antimicrobial effect of stannous fluoride on periodontitis publication-title: Journal of Clinical Periodontology doi: 10.1111/j.1600-051x.1981.tb02031.x – volume: 7 start-page: e52053 year: 2012 ident: 10.7717/peerj.10279/ref-10 article-title: Activity of 25-hydroxylase in human gingival fibroblasts and periodontal ligament cells publication-title: PLOS ONE doi: 10.1371/journal.pone.0052053 – volume: 19 start-page: E2663 year: 2018 ident: 10.7717/peerj.10279/ref-20 article-title: Vitamin D: effect on haematopoiesis and immune system and clinical applications publication-title: International Journal of Molecular Sciences doi: 10.3390/ijms19092663 – volume: 37 start-page: 199 year: 2016 ident: 10.7717/peerj.10279/ref-27 article-title: Vitamin D3 analog maxacalcitol (OCT) induces hCAP-18/LL-37 production in human oral epithelial cells publication-title: BiomedicalResearch doi: 10.2220/biomedres.37.199 – volume: 64 start-page: 307 year: 2018 ident: 10.7717/peerj.10279/ref-37 article-title: 25-Hydroxyvitamin D3 alleviates experimental periodontitis via promoting expression of cathelicidin in mice with type 2 diabetic mellitus publication-title: Journal of Nutritional Science and Vitaminology doi: 10.3177/jnsv.64.307 – volume: 55 start-page: 2140 year: 2014 ident: 10.7717/peerj.10279/ref-1 article-title: Characterization of vitamin D production by human ocular barrier cells publication-title: Investigative Ophthalmology & Visual Science doi: 10.1167/iovs.13-13019 – volume: 339 start-page: 1448 year: 2013 ident: 10.7717/peerj.10279/ref-29 article-title: Type I interferon suppresses type II interferon-triggered human anti-mycobacterial responses publication-title: Science doi: 10.1126/science.1233665 – volume: 93 start-page: 639 year: 2014 ident: 10.7717/peerj.10279/ref-36 article-title: Prospective study of serum 25-hydroxy vitamin D and tooth loss publication-title: Journal of Dental Research doi: 10.1177/0022034514534985 – volume: 49 start-page: 574 year: 2014 ident: 10.7717/peerj.10279/ref-16 article-title: Effect of non-surgical periodontal treatment on short chain fatty acid levels in gingival crevicular fluid of patients with generalized aggressive periodontitis publication-title: Journal of Periodontal Research doi: 10.1111/jre.12137 – volume: 16 start-page: 234 year: 2020 ident: 10.7717/peerj.10279/ref-3 article-title: New aspects of vitamin D metabolism and action - addressing the skin as source and target publication-title: Nature Reviews. Endocrinology doi: 10.1038/s41574-019-0312-5 – volume: 89 start-page: 294 year: 2018 ident: 10.7717/peerj.10279/ref-6 article-title: Preliminary investigation of the vitamin D pathway in periodontal connective tissue cells publication-title: Journal of Periodontology doi: 10.1002/JPER.17-0530 – volume: 20 start-page: 2417 year: 2006 ident: 10.7717/peerj.10279/ref-31 article-title: Evidence for auto/paracrine actions of vitamin D in bone: 1alpha-hydroxylase expression and activity in human bone cells publication-title: Faseb Journal doi: 10.1096/fj.06-6374fje – volume: 41 start-page: 1794 year: 2010 ident: 10.7717/peerj.10279/ref-34 article-title: Higher levels of GATA3 predict better survival in women with breast cancer publication-title: Human Pathology doi: 10.1016/j.humpath.2010.06.010 – volume: 15 start-page: 453 year: 2018 ident: 10.7717/peerj.10279/ref-4 article-title: Do dietary calcium and vitamin D matter in men with prostate cancer? publication-title: Nature Reviews Urology doi: 10.1038/s41585-018-0015-z – volume: 54 start-page: 444 year: 2019 ident: 10.7717/peerj.10279/ref-21 article-title: Activation of vitamin D in the gingival epithelium and its role in gingival inflammation and alveolar bone loss publication-title: Journal of Periodontal Research doi: 10.1111/jre.12646 – volume: 99 start-page: 1281 year: 2019 ident: 10.7717/peerj.10279/ref-23 article-title: Endothelial to mesenchymal transition: role in physiology and in the pathogenesis of human diseases publication-title: Physiological Reviews doi: 10.1152/physrev.00021.2018 – volume: 45 start-page: S162 issue: Suppl 20 year: 2018 ident: 10.7717/peerj.10279/ref-22 article-title: Periodontitis: consensus report of workgroup 2 of the 2017 world workshop on the classification of periodontal and peri-implant diseases and conditions publication-title: Journal of Clinical Periodontology doi: 10.1111/jcpe.12946 – volume: 1117 start-page: 215 year: 2019 ident: 10.7717/peerj.10279/ref-32 article-title: Design of antimicrobial peptides: progress made with human cathelicidin LL-37 publication-title: Advances in Experimental Medicin and Biology doi: 10.1007/978-981-13-3588-4_12 – volume: 17 start-page: 844 year: 2014 ident: 10.7717/peerj.10279/ref-7 article-title: Predicted vitamin D status and incidence of tooth loss and periodontitis publication-title: Public Health Nutrition doi: 10.1017/S1368980013000177 – volume: 10 start-page: E1656 year: 2018 ident: 10.7717/peerj.10279/ref-24 article-title: Vitamin D: nutrient, hormone, and immunomodulator publication-title: Nutrients doi: 10.3390/nu10111656 – volume: 45 start-page: S149 issue: Suppl 20 year: 2018 ident: 10.7717/peerj.10279/ref-30 article-title: Staging and grading of periodontitis: framework and proposal of a new classification and case definition publication-title: Journal of Clinical Periodontology doi: 10.1111/jcpe.12945 |
| SSID | ssj0000826083 |
| Score | 2.2504253 |
| Snippet | Vitamin D 1α-hydroxylase CYP27B1 is the key factor in the vitamin D pathway. Previously, we analyzed the expression of CYP27B1 in human gingival fibroblasts in... Background Vitamin D 1α-hydroxylase CYP27B1 is the key factor in the vitamin D pathway. Previously, we analyzed the expression of CYP27B1 in human gingival... |
| SourceID | pubmedcentral proquest pubmed crossref |
| SourceType | Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | e10279 |
| SubjectTerms | Antigens Biotechnology Cell Biology Connective tissues Dentistry Epithelial cells Fibroblasts Gene expression Gingiva Histology Hydroxylase Immunofluorescence Immunohistochemistry Infectious Diseases Inflammation Molecular Biology Monoclonal antibodies mRNA Periodontitis Surgery Teeth Vimentin Vitamin D |
| SummonAdditionalLinks | – databaseName: ProQuest Central Database Suite (ProQuest) dbid: BENPR link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3da9tADBdbCmMvo91XvX5wgzwNTHI--85-Kt2aEAYJYyyQN3O270jGamfNB-uf1X-kf1Ml5-Im3ZgfLYGNJEv66WQJoK0tWq4Rwpe6W_ihziI_Mfi5S6M5Kr3IRD2sejiSg3H4dRJNXMFt4doqtz6xdtRFlVONvBOEKkxiNCFxMf_t09YoOl11KzSewwG64Bjt_OBzb_Tte1NlwQAnMcnY_JinELp05sbc_KSBBdS8tRuK_sovn7ZJ7sSd_iG8cgkju9xo-AiemfI1vBi6I_E3MOz9cb2sJassWyPWv56V7Irx-zt_eltQmwpmyIbhzXohH6O1RDM0MGYRKlcZEpcLoq5n6-otjPu9H18GvtuR4OeIzJY-1znG6CKRVonIUnSx0vCCJ1FhAxkFyhQhDdgR3CZ5V2mTZxazBBtnkiODEu-gVValOQamNaJVayKuOKEsrROdixA9gFI2yLT04NNWXGnuBojTHotfKQIJkm1ayzatZetBu2Geb-Zm_JvtdCv31H08i_RR1R58bMho9nSWoUtTrYgHNYtXHHjwfqOm5jlCEOqKux6oPQU2DDRSe59Szqb1aG2l4iSW4sP_X-sEXgbU2kKVmPAUWsublTnD3GSZnTsDfADn0efs priority: 102 providerName: ProQuest |
| Title | Expression of vitamin D 1α-hydroxylase in human gingival fibroblasts in vivo |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/33505780 https://www.proquest.com/docview/2474980513 https://www.proquest.com/docview/2482666682 https://pubmed.ncbi.nlm.nih.gov/PMC7789863 |
| Volume | 9 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1LT-MwEB7xkBAXBMtjw6PySpxWCtRxYscntI-yaKUitKJSb5GT2GoRm0ApFfws_gi_aWfctNqyHDaHHOKJEs3Ynu-LnW8Ajo3DnmuFCKVpl2Fs8iTUFoe7tIZj0MtceLHq7qW86MU_-0l_CWbFOBsHPrxL7aieVG90e_J0_3yGAx7x64lCNnJ6Z-3ohjQIlF6GVUxJklhYt8H5fkpGEN32kpwR6Xwj6tDTf_Xe3r-Ynf6BnG93Tv6Vis43YaPBkOzLNOhbsGSrD7DWbVbJt6HbeWq2t1asdmyC9P_3sGLfGX99CQfPJe1cQdBsGV70NfoYVSoaYp9jDtlznWPj-IFaJ8NJvQO98871t4uwKZsQFkjWxiE3BabtUkunROIo4Thpecl1UrpIJpGyZUyaO4I7XbSVsUXuEDi4NJccDZTYhZWqruxHYMYggXU24YoT8TJGm0LEOCko5aLcyAA-z9yVFY2mOJW2uM2QW5BvM-_bzPs2gOO58d1USuN9s8OZ37NZd8iiWMU6xQlEBPBp3owjgZY3TGXrR7LBKOORRgHsTcM0f44QRMTSdgBqIYBzA1LZXmyphgOvtq1UqlMp9v_v7Q9gPaJdL_SRJj6ElfHo0R4hbBnnLVj92rm8-tXytB_PP_q85bvpH4VO8YE |
| linkProvider | Scholars Portal |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3dT9RAEJ_gkagvxE8soK4Jvpg03Hbb3fbBGJQjh3AXYyDhrW7b3VCj7cmVQ_4pE_8R_iZn-iWo8Y177Ezay8xv52N3dgZgU1tErhHClXqYub5OAjcyuNyl0RyVniWiblY9mcrxkf_-ODhegh_dXRgqq-xsYm2oszKlPfItz1d-FCKExJvZN5emRtHpajdCo4HFvrk4x5Rt_npvB_X70vN2R4fvxm47VcBNMZepXK5T9GpZJK0SgSV7bKXhGY-CzHoy8JTJfGpJI7iN0qHSJk0s-lUbJpIjgxL43luw7NON1gEsvx1NP3zsd3XQoUoMapqLgApTpa2ZMaefqUECFYtddX1_xbN_lmVe8XO792ClDVDZdoOo-7Bkigdwe9IewT-Eyeh7WztbsNKyRV7pr3nBdhi__OmeXGRUFoMRuWH4sB4AyGgMUo6AZhZT8zJBYjUn6iJflI_g6Eak9xgGRVmYJ8C0xuzYmoArTlmd1pFOhY8WRynrJVo68KoTV5y2DctpbsaXGBMXkm1cyzauZevAZs88a_p0_Jtto5N73C7WefwbWg686Mm4zOjsRBemPCMe1Cz-Qs-B1UZN_XeEoCwvHDqgrimwZ6AW3tcpRX5St_JWKoxCKdb-_7eew53x4eQgPtib7q_DXY_KamgXyN-AQXV6Zp5iXFQlz1owMvh00_j_BXY8JBI |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9QwEB6VrVRxQZRnoAUjlQtStOs4sZMDQoXdVUvZVYWo1FtwElsNgmTppgv9WVz7I_hNzORFC4hbc8yMkmjm8zzsyQzAjraIXCOEK_Uoc32dBG5kcLlLozkqPUtE3ax6Npd7R_7b4-B4DS66f2GorLKzibWhzsqU9siHnq_8KEQIiaFtyyIOx9NXi68uTZCik9ZunEYDkQNz_g3Tt-XL_THq-rnnTScf3uy57YQBN8W8pnK5TtHDZZG0SgSWbLOVhmc8CjLrycBTJvOpPY3gNkpHSps0sehjbZhIjgxK4HNvwLpCr-gPYP31ZH74vt_hQecqMcBpfgpUmDYNF8acfqJmCVQ4dtkN_hXb_lmiecnnTW_DrTZYZbsNujZhzRR3YGPWHsffhdnke1tHW7DSslVe6S95wcaM__zhnpxnVCKD0blheLMeBshoJFKO4GYW0_QyQWK1JOoqX5X34OhapHcfBkVZmIfAtMZM2ZqAK04ZntaRToWP1kcp6yVaOvCiE1ects3LaYbG5xiTGJJtXMs2rmXrwE7PvGh6dvybbauTe9wu3GX8G2YOPOvJuOToHEUXpjwjHtQsXqHnwINGTf17hKCMLxw5oK4osGegdt5XKUV-Urf1ViqMQike_f-znsIG4j5-tz8_eAw3PaqwoQ0hfwsG1emZ2cYQqUqetFhk8PG64f8LmLooPg |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Expression+of+vitamin+D+1%CE%B1-hydroxylase+in+human+gingival+fibroblasts+in+vivo&rft.jtitle=PeerJ+%28San+Francisco%2C+CA%29&rft.au=Liu%2C+Kaining&rft.au=Han%2C+Bing&rft.au=Hou%2C+Jianxia&rft.au=Zhang%2C+Jianyun&rft.date=2021-01-04&rft.issn=2167-8359&rft.eissn=2167-8359&rft.volume=9&rft.spage=e10279&rft_id=info:doi/10.7717%2Fpeerj.10279&rft.externalDBID=n%2Fa&rft.externalDocID=10_7717_peerj_10279 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2167-8359&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2167-8359&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2167-8359&client=summon |