The Relevance of Autoantigen Source and Cutoff Definition in Antichromatin (Nucleosome) Antibody Immunoassays

• Published in: Annals of the New York Academy of Sciences Vol. 1050; no. 1; pp. 176 - 184
• Main Authors: VILLALTA, DANILO, TOZZOLI, RENATO, BIZZARO, NICOLA, TONUTTI, ELIO, GHIRARDELLO, ANNA, DORIA, ANDREA
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.06.2005
• Subjects: Adult; anti-native DNA antibodies; Antibodies, Antinuclear - blood; antichromatin antibodies; antinucleosome antibodies; Arthritis, Rheumatoid - immunology; Autoantibodies - blood; autoantigen source; Autoantigens - immunology; Biomarkers - analysis; Chromatin - immunology; cutoff; Enzyme-Linked Immunosorbent Assay; Evaluation Studies as Topic; Humans; Immunoassay; Lupus Erythematosus, Systemic - diagnosis; Lupus Erythematosus, Systemic - immunology; Male; Nucleosomes - immunology; Prospective Studies; Reagent Kits, Diagnostic; receiver operating characteristic (ROC) curves; ROC Curve; Scleroderma, Systemic - immunology; Sensitivity and Specificity; Sjogren's Syndrome - immunology; systemic lupus erythematosus (SLE)

: In the last few years, several reports have shown that chromatin (nucleosome) represents the main autoantigen‐immunogen in systemic lupus erythematosus (SLE) and that specific antibodies are an important marker of the disease. To verify the clinical sensitivity and specificity of antinucleosome au...